Cytotoxicity evaluation of luting resin cements on bovine dental pulp-derived cells (bDPCs) by real-time cell analysis

Malkoç, Meral Arslan; Demir, Neclå; Şengün, Abdülkadir; Bozkurt, Şerife Buket; Hakkı, Sema S.

doi:10.4012/dmj.2014-167

Cited by 12 publications

(6 citation statements)

References 24 publications

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“…Recently, Arslan‐Malkoç et al () evaluated the cytotoxicity of different resin cements on dental pulp‐derived cells. Although different formulation has been used, RelyX U200 and Multilink Automix were cytotoxic to pulp cells and were capable of eliciting biological responses.…”

Section: Discussionmentioning

confidence: 99%

Resin luting materials: Tissue response in dog's teeth

Bezzon

Rivera

Silva

et al. 2015

Microscopy Res & Technique

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The aim of this study was to evaluate radiographically and histologically the pulpal and periapical response to self-adhesive (Rely X™ Unicem) and self-etching and self-curing (Multilink(®)) resin-based luting materials in deep cavities in dogs' teeth. Deep class V cavities (0.5-mm-thick dentin) were prepared in 60 canine premolars and the following materials were applied on cavity floor: Groups I/V-RelyX™ Unicem; Groups II/VI-Multilink(®); Groups III/VII-zinc phosphate cement (control) and; Groups IV/VIII-gutta-percha (control). Cavities were restored with silver amalgam. Animals were euthanized after 10 days (groups I-IV) and 90 days (groups V-VIII). Tooth/bone blocks were radiographed and processed for histopathological evaluation of pulp and periapical tissue response to the materials. All materials presented similar histopathological features and radiographic findings at both periods. The pulp tissue was intact. The apical and periapical regions and periodontal ligament thickness were normal. No inflammatory cells, resorption of mineralized tissue (dentin, cementum, and alveolar bone) or bacteria were observed. The lamina dura was intact and no areas of periapical bone rarefaction or internal/external root resorption were observed radiographically. It can be concluded that Rely X™ Unicem and Multilink(®) caused no adverse tissue reactions and may be indicated for cementation of indirect restorations in deep dentin cavities without pulp exposure.

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Section: Discussionmentioning

confidence: 99%

Resin luting materials: Tissue response in dog's teeth

Bezzon

Rivera

Silva

et al. 2015

Microscopy Res & Technique

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“…Rely X Veneer is composed of only two monomers: BisGMA and TEGDMA, whereas Variolink II and AllCem Veneer present an additional monomer type, UDMA and BisEMA, respectively. A current study has demonstrated that TEGDMA and BisGMA present severe cytotoxicity according to the following rank order: BisGMA>UDMA>TEGDMA>BisEMA 8 . Therefore, based on our previous results, it is possible to suggest that the presence of a third monomer in the veneer composition may decrease their cytotoxic effect.…”

Section: Discussionmentioning

confidence: 99%

“…It has been demonstrated that resin cements present toxic reaction in cell cultures by unreacted monomers released from the material during the polymerization reaction, which is related to thickness, light intensity, and photoactivation times of the material 8,12 . Is has also been demonstrated that comonomers, additives, and reaction products are released from polymerized cements 12,18 .…”

Section: Discussionmentioning

confidence: 99%

“…It is known that the greater the amount of unreacted monomers contained in a cured cement, the higher the toxic effects 1 . Furthermore, it has been reported that the nature of the matrix monomers can significantly influence the release of toxic components 8 .…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, this interface between veneers and dental structure should be regarded as a critical issue to be addressed. There is a close 25µ-thick contact between the resin cement and the oral environment, which may cause local irritation and risk to patients [6][7][8] . Resin cements are composed of a polymeric organic matrix based on methacrylate monomers, filler particles, and bonding and other chemical agents, which are responsible for the polymerization reaction 7,9 .…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxicity of three light-cured resin cements on 3T3 fibroblasts

Tumscitz

Laxe

Pascoal

et al. 2017

Rev. odontol. UNESP

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ResumoIntrodução: Cimentos resinosos fotopolimerizáveis são materiais de eleição para a cimentação de facetas laminadas. Devem ser biocompatíveis oferencendo riscos mínimos ao uso clínico em pacientes. Objetivo: O objetivo desse trabalho foi avaliar in vitro a citotoxicidade de três cimentos resinosos: Variolink II (Ivoclar Vivadent), Allcem Veneer, (FGM) e Rely X Veneer (3M ESPE). Material e método: Vinte e quatro corpos de prova de cada cimento foram confeccionados em matrizes metálicas padronizadas e inseridos em placa de cultura de células de noventa e seis poços contendo fibroblastos da linhagem 3T3. As células foram cultivadas em meio de cultivo celular RPMI 1640 com 5% de soro fetal bovino, com 0,1% de penicilina/estreptomicina em estufa a 37°C, em atmosfera úmida com 5% de CO2. O grau de citotoxicidade de cada cimento foi avaliado após os tempos de contato de 24h, 48h e 72h através do método MTT (3-(4,5-dimetiltiazol-2yl)-2,5-difenil brometo de tetrazolina), que avalia a viabilidade celular pela função mitocondrial. Após os tempos estabelecidos, as amostras foram removidas, tratadas e levadas ao espectofotômetro de microplaca para leitura da absorbância em 570nm. Resultado: O cimento Variolink apresentou em 24h viabilidade de 72,24% (±6,80), em 48h de 83,92% (± 5,26) e de 92,77% (±5,59) em 72h. Allcem Veneer apresentou viabilidade de 70,46% (± 12,91) em 24h; 85,03% (± 21,4) em 48h e 70,46% (± 12,91) em 72h. O RelyX Veneer demonstrou viabilidade de 5,06% (± 0,88) em 24h; 5,84% (± 1,18) em 48h e 6,99% (± 1,34) em 72h. Conclusão: Estes resultados demonstraram que o cimento Rely-X se apresentou significativamente mais citotóxico nas condições testadas.Descritores: Facetas dentárias; cimentos de resina; citotoxicidade; resinas compostas; sobrevivência celular. AbstractIntroduction: Light-cured resin cements are the first choice for the cementation of laminate veneers. Ideally, they should be biocompatible and offer minimum risks to patients. Objective: The aim of this study was to evaluate, in vitro, the cytotoxicity of three resin cements: Variolink II, Ivoclar Vivadent (C1), Allcem Veneer, FGM (C2), and Rely X Veneer, 3M ESPE (C3). Material and method: Twenty four samples of each of the cements were fabricated in a standardized metal mold, light activated, and transferred to a 96-well cell plate with culture of fibroblasts. After 24, 48, and 72h of incubation, cytotoxicity was assessed and cell viability was calculated by the methyl-thiazol-tetrazolium (MTT) colorimetric assay. Absorbance was measured at 570 nm using a microplate spectrophotometer. Result: The following results were found: Variolink II presented viability of 72.24% (SD 6.80) after 24h, 83.92% (SD 5.26) after 48h, and 92.77% (SD 5.59) after 72h; Allcem Veneer exhibited viability of 70.46% (SD 12.91) after 24h, 85.03% (SD 21.4) after 48h, and 70.46% (SD 12.91) after 72h; Rely X Veneer showed viability of 5.06% (SD 0.88) after 24h, 5.84% (SD 1.18) after 48h, and 6.99% (SD 1.34) after 72h. Conclusion: Under these testing conditions, Rely X Vene...

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