Cytotoxicity, genotoxicity, and metal release in patients with fixed orthodontic appliances: A longitudinal in-vivo study

Hafez, Hend; Selim, Essam Mohamed Nassef; Eid, Faten Hussein Kamel; Tawfik, Wael Attia; Al-Ashkar, Emad A.; Mostafa, Yehya A.

doi:10.1016/j.ajodo.2010.05.025

Cited by 129 publications

(106 citation statements)

References 47 publications

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“…17,[19][20][21][22][23] The results presented by Angelieri et al and Heravi et al showed that the micronucleus frequencies were not significantly different before, during and after orthodontic treatment, and are in contrast with those of Natarajan et al, who found a significantly higher MN count in the test group at the day of debonding as compared to the control group without appliances. 20,22,23 Hafez et al 19 found that the cytotoxicity and genotoxicity of orthodontic appliances remained in the mouth for 6 months. According to Natarajan et al and Hafez et al fixed orthodontic appliances emit metal ions in sufficient quantities to induce a localized genotoxic effect, but these changes were not more evident after a longer time.…”

Section: Discussioncontrasting

confidence: 53%

“…Faccioni et al and Amini et alreport a double or triple increase in the level of nickel and cobalt ions in the cells of cheek mucosa epithelium. 17,18 Statistically significant differences were observed for cobalt (Co) 17 , nickel (Ni) 17,18 and chromium (Cr) 19 . In another study, the differences between cobalt and chromium were not statistically significant, although the p-level was low (0.09 and 0.10).…”

Section: Discussionmentioning

confidence: 99%

“…19 The cells were collected before treatment Epithelial cells of the buccal mucosa were collected by gently brushing the internal part of the right and left cheeks with a wooden tongue depressor. The cells were immediately prepared for the cell viability and the comet assay.…”

Section: Resultsmentioning

confidence: 99%

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Trace metal ions release from fixed orthodontic appliances and DNA damage in oral mucosa cells by in vivo studies: A literature review

Downarowicz¹,

Mikulewicz²

2017

Adv Clin Exp Med

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An overview of professional literature referring to the release of metal ions from fixed orthodontic appliances and their influence on oral mucosa in conditions of in vivo are presented, along with a detailed analysis of the exposure of the cells of cheek mucosa epithelium to metal ions. Electronic databases (PubMed, Elsevier, Ebsco) were searched with no language restrictions. The relevant orthodontic journals and reference lists were checked for all eligible studies. A total of 38 scientific articles were retrieved in the initial search. However, only 7 articles met the inclusion criteria. Statistically significant differences in the levels of the amount of nickel ions, cobalt ions and chromium ions were observed in cells of cheek mucosa. The most biocompatible material used in the production of fixed orthodontic appliances is titanium, and the least biocompatible material is steel, which releases the largest amount of nickel and chromium. Metal ions are released from fixed orthodontic appliances only in the first phase of treatment. It is recommended to conduct further, long-term research on a larger number of patients to define the influence of using fixed orthodontic appliances and biological effect they might have on tissues.

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Section: Discussioncontrasting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

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Trace metal ions release from fixed orthodontic appliances and DNA damage in oral mucosa cells by in vivo studies: A literature review

Downarowicz¹,

Mikulewicz²

2017

Adv Clin Exp Med

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“…For other side, L929 mouse fibroblasts [8], have been shown to behave similarly to primary human gingival fibroblasts and, therefore, are a suitable in-vitro model to the toxicity test [9][10][11][12]. Taken in conjunction, these findings are aligned with the notion that the orthodontic elastics citotoxicity may influence their clinical performance [4,13] with regard to tissue biocompatibility.…”

Section: Introductionsupporting

confidence: 75%

Citotoxicity of nonlatex elastomeric ligatures of orthodontic use

et al. 2015

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The aim of this study is to evaluate cytotoxicity between polyurethane and non-latex orthodontic elastomeric ligatures. Seven elastomeric ligatures of black-coloured from different manufactures (3 non-latex, 3 polyurethane and 1 latex) were divided into 7 groups of 10 elastics each: Group UK (nonlatex, 3M Unitek), Group TP (nonlatex, TP Orthodontics), Group AO (nonlatex, American Orthodontics), Group O (Polyurethane, OrthoSource), Group M (Polyurethane, Morelli), Group TD (Polyurethane, Tecnident) and Group TC (latexControl, TP Orthodontics). The cytotoxicity essay was performed using cell cultures (L-929 line cells, mouse fibroblast) that were submitted to the cell viability test with neutral red ("dye-uptake") at 1, 2, 3, 7 and 28 days. Analysis of variance (ANOVA) with 1-way analysis followed by the Tukey post hoc test were employed (p<.05). The results showed no statistically significant differences between Groups UK, TP and AO in all experimental times (p>.05). There was a statistically differences (p<.05) between the viability cell of Groups UK, TP and AO with the Groups O, M, TD and TC at 1, 2, 3 and 7 days. There was no statistically differences (p>.05) between the groups O, M and TD at in all experimental times. It can be concluded that the nonlatex elastomeric ligatures of Unitek, TP Orthodontics, and American Orthodontics showed higher cell viability compared to others ligatures.

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“…Cell lines [7], such as L929 mouse fibroblasts [8], have been shown to behave similarly to primary human gingival fibroblasts and, therefore, are a suitable in-vitro model to test the toxicity [9][10][11][12] of products used intra-orally during orthodontic treatment [13][14][15]. In order of evaluate the biological behavior of these materials in cell culture, the Objective of the present in vitro study was to test the cytotoxicity of polyurethane and latex-free Orthodontic elastomeric chains of different manufactures.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the cytotoxicity of polyurethane and non-latex orthodontic chain elastics

et al. 2012

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Allergy caused by latex proteins has been well documented. Thus, the study of non-latex materials, is necessary. The aim of this study is to evaluate the cytotoxicity of silver-coloured orthodontic chain elastics, of polyurethane and latex-free. Nine chain elastics from different manufactures (3 latex-free and 6 polyurethane) were divided into 9 groups of 10 elastics each: Group UK (Latex-free, 3M Unitek), Group TP (Látex-free, TP Orthodontics), Group AO (Látex-free, American Orthodontics), Group O (Polyurethane, OrthoSource), Group M (Polyurethane, Morelli), Group TD (Polyurethane, Tecnident), Group UD (Polyurethane, Uniden), Group AZ (Polyurethane, Abzil) and Group AK (Polyurethane, Aditek). The cytotoxicity essay was performed using cell cultures (L-929 line cells, mouse fibroblast) that were submitted to the cell viability test with neutral red ("dye-uptake") at 1, 2, 3, 7 and 28 days. Analysis of variance (ANOVA) with multiple comparisons and Tukey's test were employed (p<.05). The results showed no statistically significant differences between Groups UK, TP and AO in all experimental times (p>.05), except between the Groups UK and TP at 28 days (p<.05). There was significant statistically difference (p<.05) between the viability of the cells in the Groups O and TD with the Groups M, AZ, AK at 1 and 2 days. The polyurethane elastics showed lower cell viability in the first 48 hours, with increase on 3rd and 7th day, and viability similar to latex-free elastics at 28 days. It can be concluded that the latex-free chain elastic showed higher cell viability. The OrthoSource and Tecnident trademark showed lower cell viability the initial 48 hours.

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Cytotoxicity, genotoxicity, and metal release in patients with fixed orthodontic appliances: A longitudinal in-vivo study

Cited by 129 publications

References 47 publications

Trace metal ions release from fixed orthodontic appliances and DNA damage in oral mucosa cells by in vivo studies: A literature review

Trace metal ions release from fixed orthodontic appliances and DNA damage in oral mucosa cells by in vivo studies: A literature review

Citotoxicity of nonlatex elastomeric ligatures of orthodontic use

Evaluation of the cytotoxicity of polyurethane and non-latex orthodontic chain elastics

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