Cytotoxicity in MCF-7 and MDA-MB-231 Breast Cancer Cells, without Harming MCF-10A Healthy Cells

Moses, Sherita L.; Vm, Edwards; Brantley, Eileen

doi:10.4172/2157-7439.1000369

Cited by 14 publications

(5 citation statements)

References 17 publications

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“…The most recent study used gold nanoparticles in breast cancer cells to deliver Commiphora myrrha and Boswellia sacra extracts to induce trisodium citrate dihydrate reduction which leads to cytotoxicity in breast cancer and normal cells. The study reported cytotoxicity in breast cancer cells, but no harm in normal breast cells [35].…”

Section: Introductionmentioning

confidence: 99%

A Perspective Review on the Role of Nanomedicine in the Modulation of TNF-TNFR2 Axis in Breast Cancer Immunotherapy

Al-Hatamleh

Ahmad

Boer

et al. 2019

Journal of Oncology

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In the past decade, nanomedicine research has provided us with highly useful agents (nanoparticles) delivering therapeutic drugs to target cancer cells. The present review highlights nanomedicine applications for breast cancer immunotherapy. Recent studies have suggested that tumour necrosis factor (TNF) and its receptor 2 (TNFR2) expressed on breast cancer cells have important functional consequences. This cytokine/receptor interaction is also critical for promoting highly immune-suppressive phenotypes by regulatory T cells (Tregs). This review generally provides a background for nanoparticles as potential drug delivery agents for immunomodulators and further discusses in depth the potential of TNF antagonists delivery to modulate TNF-TNFR2 interactions and inhibit breast cancer progression.

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Section: Introductionmentioning

confidence: 99%

A Perspective Review on the Role of Nanomedicine in the Modulation of TNF-TNFR2 Axis in Breast Cancer Immunotherapy

Al-Hatamleh

Ahmad

Boer

et al. 2019

Journal of Oncology

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“…Myrrh oil has 20 components that were considered. The highest component discovered in this oil was Diethyl Phthalate about 75.51% of the oil contents, which is used in industrial products such as insecticides, repellents, and camphor substitutes (Morteza-Semnani and Saeedi, 2003;Hanuš et al, 2005;Obiezue et al, 2014;Moses et al, 2016;Perveen et al, 2018). Also, many researchers found the major component of frankincense oil was alpha-Thujene (Palvela et al, 2021;Kiran et al, 2017).…”

Section: Discussionmentioning

confidence: 97%

The antifeedant properties of bio-oil from Cupressus sempervirens against rice weevil (Sitophilus oryzae) compared to that of Myrrh and Frankincense oils

Wahba

Aly²,

Hassan

2023

Egyptian Journal of Agricultural Research

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Bio-oils are a complex material that is produced through the condensation of vapors raised from the pyrolysis of plant biomass components and consist mainly of phenolic compounds, benzene derivatives, and organic acids. Few studies have been conducted on the antifeedant efficiency of bio-oils. This study aims to evaluate the chemical composition, toxicity, and antifeedant activity of bio-oil produced from the pruning residue of Cupressus sempervirens in addition to two types of essential oils, Myrrh and Frankincense oil, against Sitophilus oryzae. The GC/MS analysis of the essential oils showed Diethyl Phthalate (75.15%) in myrrh oil, alpha-Thujene (30.70%), and Octyl acetate (20.44%) in frankincense oil. The results clearly showed the high toxicity of essential oils, myrrh and frankincense, and the low toxicity of bio-oil against S. oryzae after 7 days of exposure time, while the while the combined use of myrrh oil and frankincense oil with bio-oil increased the toxicity against S. oryzae about 1.91 and 2.27-fold, compared to myrrh oil and frankincense oil alone. In all treatments, the antifeedant parameters revealed that myrrh oil had higher nutritional indices than Frankincense oil. C. sempervirens showed a significantly low antifeedant efficacy compared to both essential oils. Generally, the values of relative growth rate (RGR), relative consumption rate (RCR), and efficiency of conversion of ingested food, (ECI) significantly decreased, and, contrariwise, the FDI increased in all treatments compared to the control.

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“…The experimental data indicated that proteins in Cuscuta crude and GFC peaks suppress the viability of MCF-7 breast cancer cells in a dose-dependent manner, while the viability of normal epithelial breast cancer cells MCF-10 A remains unaffected. The non-cytotoxic effect on estrogen negative MCF-10 A cells suggests that Cuscuta proteins produce promising selective cytotoxicity in estrogen receptor positive breast cancer cells [ 38 ]. However, the crude and GFC peaks appeared to display little variation in the extent of cytotoxic activity.…”

Section: Discussionmentioning

confidence: 99%

Proteomic characterization and cytotoxic potential of proteins from Cuscuta (Cuscuta epithymum (L.) crude herbal product against MCF-7 human breast cancer cell line

Akhtar,

Khurshid,

El-Aarag

et al. 2024

BMC Complement Med Ther

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Background The burden of breast cancer, the second leading cause of death worldwide, is increasing at an alarming rate. Cuscuta, used in traditional medicine for different ailments, including cancer, is known for containing phytochemicals that exhibit anticancer activity; however, the bioactivities of proteins from this plant remain unexplored. This study aimed to screen the cytotoxic potential of proteins from the crude herbal product of Cuscuta epithymum(L.) (CE) harvested from the host plants Alhagi maurorum and Medicago sativa. Methods The proteins from CE were extracted using a salting-out method, followed by fractionation with a gel filtration chromatography column. Gel-free shotgun proteomics was subsequently performed for protein characterization. The viability assay using MTT was applied to deduce the cytotoxic potential of proteins against MCF-7 breast cancer cells, with further exploration of the effect of treatment on the expression of the apoptotic mediator BCL2-associated X protein (BAX) and B-cell lymphoma protein 2 (BCL-2) proteins, using western blotting to strengthen the findings from the in vitro viability assay. Results The crude proteins (CP) of CE were separated into four protein peaks (P1, P2, P3, and P4) by gel filtration chromatography. The evaluation of potency showed a dose-dependent decline in the MCF-7 cell line after CP, P1, P2, and P3 treatment with the respective IC50 values of 33.8, 43.1, 34.5, and 28.6 µg/ml. The percent viability of the cells decreased significantly upon treatment with 50 µg/ml CP, P1, P2, and P3 (P < 0.001). Western-blot analysis revealed upregulation of proapoptotic protein BAX in the cells treated with CP, P3 (P < 0.01), and P2 (P < 0.05); however, the antiapoptotic protein, BCL-2 was downregulated in the cells treated with CP and P3 (P < 0.01), but no significant change was detected in P2 treated cells. The observed cytotoxic effects of proteins in the CP, P1, P2, and P3 from the in vitro viability assay and western blot depicted the bioactivity potential of CE proteins. The database search revealed the identities of functionally important proteins, including nonspecific lipid transfer protein, superoxide dismutase, carboxypeptidase, RNase H domain containing protein, and polyribonucleotide nucleotidyltransferase, which have been previously reported from other plants to exhibit anticancer activity. Conclusion This study indicated the cytotoxic activity of Cuscuta proteins against breast cancer MCF-7 cells and will be utilized for future investigations on the mechanistic effect of active proteins. The survey of CE proteins provided substantial data to encourage further exploration of biological activities exhibited by proteins in Cuscuta.

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Cytotoxicity in MCF-7 and MDA-MB-231 Breast Cancer Cells, without Harming MCF-10A Healthy Cells

Cited by 14 publications

References 17 publications

A Perspective Review on the Role of Nanomedicine in the Modulation of TNF-TNFR2 Axis in Breast Cancer Immunotherapy

A Perspective Review on the Role of Nanomedicine in the Modulation of TNF-TNFR2 Axis in Breast Cancer Immunotherapy

The antifeedant properties of bio-oil from Cupressus sempervirens against rice weevil (Sitophilus oryzae) compared to that of Myrrh and Frankincense oils

Proteomic characterization and cytotoxic potential of proteins from Cuscuta (Cuscuta epithymum (L.) crude herbal product against MCF-7 human breast cancer cell line

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