Cytotoxicity of 40 Egyptian plant extracts targeting mechanisms of drug-resistant cancer cells

Efferth, Thomas; Abdelfatah, Sara; Hamed, Ahmed R.; Mohamed, Tarik A.; Elshamy, Abdelsamed I.; Saleh, Ibrahim A.; Reda, Eman H.; Abdel-Azim, Nahla S.; Shams, Khaled A.; Sakr, Mohamed; Sugimoto, Yoshikazu; Paré, Paul W.; Efferth, Thomas

doi:10.1016/j.phymed.2018.11.031

Cited by 42 publications

(17 citation statements)

References 40 publications

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“…Asteraceae is one of the highest represented plant families in Sinai at over 15% based on species numbers. In a continuing investigation of secondary metabolites from natural sources, 4‐10 here we compare essential oils extraction protocols for Asteraceae species abundant in Sinai.…”

Section: Introductionmentioning

confidence: 99%

Extraction development for antimicrobial and phytotoxic essential oils from asteraceae species: Achillea fragrantissima, Artemisia judaica and Tanacetum sinaicum

Elshamy

Abd‐ElGawad

Mohamed

et al. 2021

Flavour & Fragrance J

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The Egyptian Sinai is a rich source of plant‐derived medicines from the highly represented Asteraceae family; however, the protocol for effective chemical extraction has not been standardized. To identify an effective extraction method for essential oils for highly represented species, Achillea fragrantissima, Artemisia judaica and Tanacetum sinaicum were analysed by hydro‐distillation (HD) vs microwave‐assisted (MA) extraction. A total of 82 metabolites were identified by gas chromatography‐mass spectrometry (GC‐MS). While oxygenated monoterpenes were the major class for both methods, MA extraction proved to be slightly more effective in extracting some ketones and alcohols such as piperitone and thymol, respectively. All the extracted EOs from the three plants exhibited significant antimicrobial potentialities in which T sinaicum ˃ A judaica > A fragrantissima. Interestingly, essential oils extracted by HD (vs MAE) exhibited greater lettuce phytotoxicity with respect to root and shoot growth as well as seed germination. Although the two extraction methods produce slightly different chemical profiles for the three analysed species, the plants aggregated similarly based on principal component analysis.

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Section: Introductionmentioning

confidence: 99%

Extraction development for antimicrobial and phytotoxic essential oils from asteraceae species: Achillea fragrantissima, Artemisia judaica and Tanacetum sinaicum

Elshamy

Abd‐ElGawad

Mohamed

et al. 2021

Flavour & Fragrance J

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“…Nuclear magnetic resonance (NMR) spectra were recorded on a Bruker 300 NMR spectrometer (Bruker Corporation). Reversed phase high-performance liquid chromatography (HPLC)-mass spectrometry (MS) analysis was performed on a Waters Alliance 2695 LC (Waters Corporation) coupled to a Quattro Ultima triple quadrupole MS (Waters Corporation) using the same separation conditions as described previously (46). The separation conditions were follow: Chromatogram column, XBridge™ column (4.6×150 mm, 5 µ m); column temperature, 20°C; and injection volume, 1 µ l. Elution was performed at a flow rate of 1 ml/min, using as the mobile phase a mixture of water (A) and acetonitrile (B).…”

Section: Methodsmentioning

confidence: 99%

Retrospective study of small pet tumors treated with Artemisia annua and iron

Saeed

Breuer²,

Hegazy

2019

Int J Oncol

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Artemisinin from Artemisia annua L. and its derivatives are well-known antimalarial drugs. In addition, in vitro studies, in vivo studies and clinical trials have demonstrated that these drugs exhibit anticancer activity in human patients with cancer. Therefore, the aim of the present study was to investigate whether a phytotherapeutic A. annua preparation exerts anticancer activity in veterinary tumors of small pets. Dogs and cats with spontaneous cancer (n=20) were treated with standard therapy plus a commercial A. annua preparation (Luparte®) and compared with a control group treated with standard therapy alone (n=11). Immunohistochemical analyses were performed with formalin-fixed paraffin-embedded tumor biopsies to analyze the expression of transferrin receptor (TfR) and the proliferation marker Ki-67 as possible biomarkers to assess treatment response of tumors to A. annua. Finally, the expression levels of TfR and Ki-67 were compared with the IC50 values towards artemisinin in two dog tumor cells lines (DH82 and DGBM) and a panel of 54 human tumor cell lines. Retrospectively, the present study assessed the survival times of small animals treated by standard therapy with or without A. annua. A. annua treatment was associated with a significantly higher number of animals surviving >18 months compared with animals without A. annua treatment (P=0.0331). Using a second set of small pet tumors, a significant correlation was identified between TfR and Ki-67 expression by immunohistochemistry (P=0.025). To further assess the association of transferrin and Ki-67 expression with cellular response to artemisinin, the present study compared the expression of these two biomarkers and the IC50 values for artemisinin in National Cancer Institute tumor cell lines in vitro. Both markers were inversely associated with artemisinin response (P<0.05), and the expression levels of TfR and Ki-67 were significantly correlated (P=0.008). In conclusion, the promising results of the present retrospective study warrant further confirmation by prospective studies in the future.

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“…Worldwide, there are at least 50 000 plant species used for medicinal effect 4 against various diseases, including cancer 5‐7 . In recent decades, there has been a growing interest in biomolecules in cancer research 8,9 . Plant extracts may be used to increase therapeutic efficacy by sensitizing malignant cells to drugs such as hematologic malignancies 1,3,5,10 …”

Section: Introductionmentioning

confidence: 99%

Hydromethanolic root and aerial part extracts from Echium arenarium Guss suppress proliferation and induce apoptosis of multiple myeloma cells through mitochondrial pathway

Limam

Aissa-Fennira

Chahbi

et al. 2021

Environmental Toxicology

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Echium arenarium Guss is a Mediterranean plant traditionally used in healing skin wound and it was reported exhibiting potent antioxidant, antibacterial, and antiparasitic activities. However, antitumoral activities of this plant have not yet been explored. Here we investigated for the first time, root (EARE) and aerial part (EAAPE) extracts of E. arenarium Guss to examine cytotoxicity and apoptosis activation pathway on U266 human multiple myeloma (MM) cell line. We demonstrated that EARE and EAAPE decreased U266 cell viability in a dose dependent manner. Based on 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay, EARE was significantly two times more efficient (IC50 value 41 μg/ml) than EAAPE (IC50 value 82 μg/ml) considering 48 h of treatment. Furthermore, after 24 h of exposure to 100 μg/ml of EARE or EAAPE, cell cycle showed remarkable increase in sub‐G1 population and a decrease of U266 cells proportion in G1 phase. In addition, EARE increased cell percentage in S phase. Moreover, analysis revealed that EAAPE or EARE induced apoptosis of U266 cells after 24 h of treatment. Interestingly, depolarization of mitochondrial membrane potential and activation of caspase 3/7 were demonstrated in treated U266 cells. Phytochemical analysis of E. arenarium extracts showed that EARE exhibited the highest content of total phenolic content. Interestingly, six phenolic compounds were identified. Myricitrin was the major compound in EARE, followed by luteolin 7‐O‐glucoside, resorcinol, polydatin, Trans‐hydroxycinnamic acid, and hyperoside. These findings proved that an intrinsic mitochondria‐mediated apoptosis pathway probably mediated the apoptotic effects of E. arenarium Guss extracts on U266 cells, and this will suggest several action plans to treat MM.

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Cytotoxicity of 40 Egyptian plant extracts targeting mechanisms of drug-resistant cancer cells

Cited by 42 publications

References 40 publications

Extraction development for antimicrobial and phytotoxic essential oils from asteraceae species: Achillea fragrantissima, Artemisia judaica and Tanacetum sinaicum

Extraction development for antimicrobial and phytotoxic essential oils from asteraceae species: Achillea fragrantissima, Artemisia judaica and Tanacetum sinaicum

Retrospective study of small pet tumors treated with Artemisia annua and iron

Hydromethanolic root and aerial part extracts from Echium arenarium Guss suppress proliferation and induce apoptosis of multiple myeloma cells through mitochondrial pathway

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