Cytotoxicity of a new hemostatic agent on human pulp fibroblasts in vitro

Odabaş, Mesut Enes; Ertürk, Murat; Çınar, Çağdaş; Tüzüner, Tamer; Tulunoğlu, Özlem

doi:10.4317/medoral.16.e584

Cited by 30 publications

(27 citation statements)

References 23 publications

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“…Therefore, there are molecular links underlying ABS-pleiotropic cellular actions acting on anti-infective [30][31][32] , wound healing 2,[33][34][35][36] , and apoptotic processes. 22,23 Since myeloma is located at the crossroads of cellular neoplastic development and M-protein secretion, the unique effects of ABS on distinct clonal paraproteins shall be investigated in future in vivo animal models mimicking plasma cell disorders. Our present study represent true basis for the design of such trials.…”

Section: Discussionmentioning

confidence: 99%

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Striking Promotion of the in vitro Myeloma Monoclonal Immunoglobulin Aggregation by Ankaferd Hemostat

Albayrak¹

2012

UHOD

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Ankaferd Blood Stopper (ABS) is a novel topical hemostatic agent. ABS-induced formation of the protein network with vital erythroid aggregation covers the entire physiological hemostatic process. ABS has pleiotropic cellular, proteomic, transcriptomic, and metabolomic effects. ABS also affects the expression of important hemostatic molecules namely PAR-1, EPCR and PAI-1. The aim of this study was to detect the macroscopic, biochemical, and cytopathological effects of ABS on myeloma monoclonal immunoglobulin (M-protein). Based on our results, the addition of ABS into the serum of both multiple myeloma (MM) and control groups resulted in significantly decrements in the level of total protein, albumin, IgG, IgA and IgM. Furthermore, the decrements in the MM patients were more pronounced than in the healthy control subjects. ABS has a potential role in decreasing of serum proteins and monoclonal M-proteins. Moreover, the declining in the neoplastic monoclonal M-protein was more prominent. We hypothesized that ABS could be used as an "agglutination-controlling factor" for myeloma monoclonal proteins and the protein-aggregating effects of ABS may be helpful for expressing the regulatory molecules promoting or preventing the myeloma protein aggregation.

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Section: Discussionmentioning

confidence: 99%

“…[1][2][3][15][16][17][18][19][20] ABS has many cellular effects. 1,[21][22][23] ABS has been shown to affect renal tubular apoptosis based on the level of hemorrhage. 22 When the bleeding associated with the surgery of partial nephrectomy is mild or moderate, ABS can initially increase renal tubular apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Striking Promotion of the in vitro Myeloma Monoclonal Immunoglobulin Aggregation by Ankaferd Hemostat

Albayrak¹

2012

UHOD

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“…Therefore, there are molecular links underlying ABSpleiotropic cellular actions acting on anti-infective, wound healing, and apoptotic processes. 1,6,7,10,13,17,[24][25][26][27][28][29][30][31][32] Since renal system is located at the crossroads of cellular neoplastic development and protein secretions, the unique effects of ABS on distinct renal cell types shall be investigated in future in vivo animal models mimicking cancer models. Our present study represent true basis for the design of such experimental animal trials.…”

Section: Discussionmentioning

confidence: 99%

“…16 Moreover, a recent study suggested in vitro ABS cytotoxicity on the human pulp fibroblasts. 17 Therefore, ABS effects on the tissue level should also be searched after its topical usage in the injury models. The aim of this experimental study was to assess short-and mid-term topical kidney safety following the topical administration of ABS in rats.…”

mentioning

confidence: 99%

Histopathological Alterations in the Kidney Tissue Following Topical Ankaferd Hemostat Application in a Rat Renal Injury Model

Karakoç¹,

Akar²,

Aksu³

et al. 2012

UHOD

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Ankaferd Blood Stopper (ABS) is a novel topical hemostatic agent. ABS has been approved in Turkey for clinical hemorrhages, when the conventional control of bleeding by ligature and/or conventional hemostatic measures is ineffective. ABS has many cellular effects and could modulate numerous hemostatic proteins at the tissue and blood. ABS-induced formation of the protein network with vital erythroid aggregation covers the entire physiological hemostatic process. The aim of this study was to assess histopathological alterations due to topical ABS administration at the renal tissue level. Thirty-six Wistar rats weighing 70 to 80 gm were included into the study. The rats were divided into two groups as "the ABS-applied group" (ABS−G) and "the control group" (C−G). The animals in both groups were then again divided into the three subgroups of "postoperative (Postop.) 60th minutes", "Postop. 48th hours", and "Postop. 15th day". Therefore, there were six rats in each of the subgroups at the end of the analyses. The standard renal injury sites in the rats of ABS−G were applied 2 ml. of topical ABS, whereas 2 ml. of topical saline was applied to the renal injury sites of the rats in the C−G group. We detected significant erythrocyte aggregation and the accumulation of siderophages in the kidney tissue just after 60 minutes of ABS application persisting over 15 days. Our results indicated red blood cell accumulation and siderophages following the use of ABS are compatible with the suggested 'mechanism-of-action' of ABS that ABS-induced formation of the protein network with vital erythroid aggregation covers the entire physiological hemostatic process. Further experimental search is needed to find out the molecules inside the ABS protein library leading to the ABS-induced aggregation at the renal tissue level.

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“…In a recent study, Odabaş et al [27] found that ABS was cytotoxic to human pulp fibroblasts. The sensitivity of cytotoxicity depended on the concentration of the material tested.…”

Section: Ankaferd Blood Stoppermentioning

confidence: 97%

The histopathological investigation of the effect on regional and systemic tissues of the application of Medicinal Plant Extract Ankaferd Blood Stopper in deep tissue injuries in Rats

Okumuş¹,

Yüksel²,

Özbağ³

et al. 2013

Ulus Travma Acil Cerrahi Derg

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Cytotoxicity of a new hemostatic agent on human pulp fibroblasts in vitro

Cited by 30 publications

References 23 publications

Striking Promotion of the in vitro Myeloma Monoclonal Immunoglobulin Aggregation by Ankaferd Hemostat

Striking Promotion of the in vitro Myeloma Monoclonal Immunoglobulin Aggregation by Ankaferd Hemostat

Histopathological Alterations in the Kidney Tissue Following Topical Ankaferd Hemostat Application in a Rat Renal Injury Model

The histopathological investigation of the effect on regional and systemic tissues of the application of Medicinal Plant Extract Ankaferd Blood Stopper in deep tissue injuries in Rats

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