Cytotoxicity of Bio-Synthesized MgFe2O4@Ag Nanocomposite on Gastric Cancer Cell Line and Evaluation Its Effect on Bax, p53 and Bcl-2 Genes Expression

Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles

“…Silver-containing nanoparticles were shown to be toxic to cancer cells including gastric adenocarcinoma (AGS) [ 74 ], cervical cancer (HeLa) [ 75 ], prostate cancer (PC3) [ 76 ], colon cancer (HCT116) [ 77 ], and two human hormone-dependent breast cancer, T47D [ 78 , 79 ] and MCF-7 cells [ 80 ]. The flow cytometry analysis showed that treatment with silver-containing nanoparticles caused the T47D cell population to shift from the viable to the apoptotic phase [ 79 ].…”

“…The flow cytometry analysis showed that treatment with silver-containing nanoparticles caused the T47D cell population to shift from the viable to the apoptotic phase [ 79 ]. In the AGS cells, silver nanoparticle-containing drugs induced apoptosis via the p53 , Bax/BCL-2 , and caspase pathways [ 74 ].…”

Nanoparticles from Microalgae and Their Biomedical Applications

“…Similarly, Ga in GaFe2O4@Ag NCs can alter iron metabolism, resultin appearance of chromatin fragmentation and apoptotic bodies that induce cell ap The prepared PtFe2O4@Ag NCs displayed a more prominent anticancerous activ the GaFe2O4@Ag NCs; however, it was lower than cisplatin, a gastric cancer me used as a control. Furthermore, MgFe2O4@Ag NCs were synthesized using an e C. vulgaris showing anticancer activity through a similar apoptotic pathway [56]. The influence of various nanocomposites (NCs) synthesized from microalgae on cancer cell lines was also tested.…”

“…The prepared PtFe 2 O 4 @Ag NCs displayed a more prominent anticancerous activity than the GaFe 2 O 4 @Ag NCs; however, it was lower than cisplatin, a gastric cancer medication used as a control. Furthermore, MgFe 2 O 4 @Ag NCs were synthesized using an extract of C. vulgaris showing anticancer activity through a similar apoptotic pathway [ 56 ]. The role of MgO in the NCs was attributed to the enhancement of the magnetic properties and disruption of the cell membrane.…”

Evaluation of the cytotoxic effect of GaFe2O4@Ag magnetic nanocomposite biosynthesized by Spirulina platensis on breast cancer cell line and evaluation of BAX, Bcl-2, CASP9 and MMP1 genes expression

“…Sequence (5'-3') Reference CASP9-forwars CCAGATGCCACCCCGTT [32] CASP9-reverse CCCACTGCTCAAAGATGTCGT BAX-forward TTGCTTCAGGGTTTCATCCA [33] BAX-reverse AGACACTCGCTCAGCTTCTTG Bcl-2-forward TGGCCAGGGTCAGAGTTAAA [34] Bcl-2-reverse TGGCCTCTCTTGCGGAGTA GAPDH-forward CCCACTCCTCCACCTTTGAC [34] GAPDH-reverse CATACCAGGAAATGAGCTTGACAA MMP1-forward CTCAGGATGACATTGATGGC [35] MMP1-reverse TGATCTTGACCAGAATACCATCGA…”