Cytotoxicity of carbobenzoxy-protected amino acids

Lelkes, Peter I.

doi:10.1007/bf02631394

Cited by 1 publication

(3 citation statements)

References 9 publications

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“…Histological evidence of such an adventitious/axillary origin of multiple shoots has been reported in legumes including chickpea (Polisetty et al 1997). Brandt and Hess (1994) reported multiple shoot formation in cotyledonary nodal explants by using 4.4 lM BAP with B5 medium. However, these experiments did not provide any information on the longterm maintenance of the shoot formation ability of the embryo axis.…”

Section: Resultsmentioning

confidence: 93%

“…The most probable reason for such a low frequency could be ascribed to the fact that the regenerants were obtained from pre-existing meristems resulting in chimeric T 0 plants. In vitro production of multiple shoots from seed/cotyledon explants has been reported in several studies (Mc Kentlyet et al 1989;Gulati and Jaiwal 1994;Prakash et al 1994;Brandt and Hess 1994). In chickpea, thidiazuron (TDZ) was shown to induce multiple shoots (Singh et al 2002).…”

Section: Introductionmentioning

confidence: 94%

“…In chickpea, thidiazuron (TDZ) was shown to induce multiple shoots (Singh et al 2002). Benzylaminopurine (BAP) at a high concentration was also shown to be effective in inducing multiple shoots in chickpea (Polisetty et al 1997;Brandt and Hess 1994). However, long-term maintenance of shoot formation ability was not attempted in those experiments.…”

Section: Introductionmentioning

confidence: 96%

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Glutamine improves shoot morphogenesis in chickpea (Cicer arietinum L.)

et al. 2009

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The use of glutamine has been shown to increase the frequency of organogenesis and regeneration in the in vitro culture of several plants. The effect of glutamine on hormone-induced multiple shoot formation in desi and kabuli genotypes of chickpea (C-235 and PUSA-1053) were evaluated. Embryo axes with or without attached cotyledons were cultured in thidiazuron (TDZ) or 6-benzylaminopurine (BAP)-containing medium, respectively, with various concentrations of glutamine. Glutamine improved and prolonged the multiple shoot regeneration ability of the embryo axis. Chickpea embryo axis with attached cotyledon and cultured in TDZ-containing medium showed improved and prolonged shoot regeneration with 5 mM glutamine, while embryo axis without cotyledon and cultured in BAP-containing medium showed prolonged regeneration ability in 10 mM glutamine. Glutamine, however, did not serve as a substitute for cotyledon. Desi genotype (C-235) showed better response for multiple shoot formation as compared to the kabuli genotype (PUSA-1053). Glutamine at a concentration of 5 mM also improved root formation in excised in vitro shoots.

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Section: Resultsmentioning

confidence: 93%