Cytotoxicity of carboxylic acid functionalized single wall carbon nanotubes on the human intestinal cell line Caco-2

Jos, Ángeles; Pichardo, Silvia; Puerto, María; Sánchez, Elena; Grilo, Antonio; Cameán, Ana M.

doi:10.1016/j.tiv.2009.07.001

Cited by 92 publications

(61 citation statements)

References 40 publications

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“…36 In contrast, Jos et al reported Caco-2 plasma membrane damage from f SWCNT-COOH exposure. 37 This discrepancy is likely due to a much lower content (,1%) of metal contaminants in our functionalized CNMs samples as compared to that used by Jos et al of 5%-8%. 37 The removal of metal contaminants, known to induce cellular oxidative stress, has been shown to mitigate the cytotoxic effect of carbon nanotubes.…”

Section: R-123 Accumulation Studies In Caco-2 Cellsmentioning

confidence: 62%

“…37 This discrepancy is likely due to a much lower content (,1%) of metal contaminants in our functionalized CNMs samples as compared to that used by Jos et al of 5%-8%. 37 The removal of metal contaminants, known to induce cellular oxidative stress, has been shown to mitigate the cytotoxic effect of carbon nanotubes. 38 It is interesting to note that the differences in morphologies and surface modifications of the three functionalized CNMs evaluated in our study did not give rise to a difference in Caco-2 cytotoxicity upon short-term (24 hour) exposure but rather a differential in cytotoxicity upon long-term (72 hour) exposure and their ability to modulate the barrier function of Caco-2 cell monolayer.…”

Section: R-123 Accumulation Studies In Caco-2 Cellsmentioning

confidence: 62%

See 1 more Smart Citation

Functionalized carbon nanomaterials: exploring the interactions with Caco-2 cells for potential oral drug delivery

Coyuco¹,

Liu²,

Tan³

et al. 2011

IJN

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Although carbon nanomaterials (CNMs) have been increasingly studied for their biomedical applications, there is limited research on these novel materials for oral drug delivery. As such, this study aimed to explore the potential of CNMs in oral drug delivery, and the objectives were to evaluate CNM cytotoxicity and their abilities to modulate paracellular transport and the P-glycoprotein (P-gp) efflux pump. Three types of functionalized CNMs were studied, including polyhydroxy small-gap fullerenes (OH-fullerenes), carboxylic acid functionalized single-walled carbon nanotubes ( f SWCNT-COOH) and poly(ethylene glycol) functionalized single-walled carbon nanotubes ( f SWCNT-PEG), using the well-established Caco-2 cell monolayer to represent the intestinal epithelium. All three CNMs had minimum cytotoxicity on Caco-2 cells, as demonstrated through lactose dehydrogenase release and 3-(4,5-dimethyliazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Of the three CNMs, f SWCNT-COOH significantly reduced transepithelial electrical resistance and enhanced transport of Lucifer Yellow across the Caco-2 monolayer. Confocal fluorescence microscopy showed that f SWCNT-COOH treated cells had the highest perturbation in the distribution of ZO-1, a protein marker of tight junction, suggesting that f SWCNT-COOH could enhance paracellular permeability via disruption of tight junctions. This modulating effect of f SWCNT-COOH can be reversed over time. Furthermore, cellular accumulation of the P-gp substrate, rhodamine-123, was significantly increased in cells treated with f SWCNT-COOH, suggestive of P-gp inhibition. Of note, f SWCNT-PEG could increase rhodamine-123 accumulation without modifying the tight junction. Collectively, these results suggest that the functionalized CNMs could be useful as modulators for oral drug delivery, and the differential effects on the intestinal epithelium imparted by different types of CNMs would create unique opportunities for drug-specific oral delivery applications.

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Section: R-123 Accumulation Studies In Caco-2 Cellsmentioning

confidence: 62%

Section: R-123 Accumulation Studies In Caco-2 Cellsmentioning

confidence: 62%

Functionalized carbon nanomaterials: exploring the interactions with Caco-2 cells for potential oral drug delivery

Coyuco¹,

Liu²,

Tan³

et al. 2011

IJN

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“…74 Asit uygulamasıyla oluş-turulmuş SWCNT yüzeyinde fonksiyonel grup (carboxyl ve hydoxyl) yoğunluğundan dolayı, saflaştırılmış SWCNTs, modifiye edilmemiş SWCNTs'den çok daha sitotoksiktir. 81 CNTs/CNFs'de toksisitesinin birinci kaynağı kobalt, nikel gibi katalizör metal artıklarından gelir. 82 Eğer saf ürünler kullanılırsa bu metallerin biyolojik sistemler için toksik olduğu bilinir.…”

Section: Antimikrobiyal Nanopartikül Metallerunclassified

Sabi̇t Ve Hareketli̇ Protezi̇n Yeni̇den Yapimi İle Esteti̇k Ve Fonksi̇yonun İyi̇leşti̇ri̇lmesi̇: Olgu Sunumu

Oyar¹

2015

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

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“…A wide array of cell types have been used to characterize the cytotoxicity of CNTs, and the data have been inconsistent and inconclusive. [50][51][52] This is mainly due to the lack of standard and customized techniques available to assess CNT toxicity. 53 For example, use of different cell types and diverse doses of CNTs for each study by different research groups has only led to a lack of consensus on this issue.…”

Section: Nanotube Biocompatibilitymentioning

confidence: 99%

“…50 This can be attributed to the carboxyl and hydroxyl groups on the CNT surface. It can, therefore, be reemphasized that rendering CNTs soluble in aqueous solutions does not guarantee the biocompatibility of the CNTs.…”

mentioning

confidence: 99%

Stealth nanotubes: strategies of shielding carbon nanotubes to evade opsonization and improve biodistribution

Kotagiri¹,

Kim²

2014

IJN

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Carbon nanotubes (CNTs) have recently been in the limelight for their potential role in disease diagnostics and therapeutics, as well as in tissue engineering. Before these medical applications can be realized, there is a need to address issues like opsonization, phagocytosis by macrophages, and sequestration to the liver and spleen for eventual elimination from the body; along with equally important issues such as aqueous solubility, dispersion, biocompatibility, and biofunctionalization. CNTs have not been shown to be able to evade such biological obstacles, which include their nonspecific attachments to cells and other biological components in the bloodstream, before reaching target tissues and cells in vivo. This will eventually determine their longevity in circulation and clearance rate from the body. This review article discusses the current status, challenges, practical strategies, and implementations of coating CNTs with biocompatible and opsonin-resistant moieties, rendering CNTs transparent to opsonins and deceiving the innate immune response to make believe that the CNTs are not foreign. A holistic approach to the development of such “stealth” CNTs is presented, which encompasses not only several biophysicochemical factors that are not limited to surface treatment of CNTs, but also extraneous biological factors such as the protein corona formation that inevitably controls the in vivo fate of the particles. This review also discusses the present and potential applications, along with the future directions, of CNTs and their hybrid-based nanotheranostic agents for multiplex, multimodal molecular imaging and therapy, as well as in other applications, such as drug delivery and tissue engineering.

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Cytotoxicity of carboxylic acid functionalized single wall carbon nanotubes on the human intestinal cell line Caco-2

Cited by 92 publications

References 40 publications

Functionalized carbon nanomaterials: exploring the interactions with Caco-2 cells for potential oral drug delivery

Functionalized carbon nanomaterials: exploring the interactions with Caco-2 cells for potential oral drug delivery

Sabi̇t Ve Hareketli̇ Protezi̇n Yeni̇den Yapimi İle Esteti̇k Ve Fonksi̇yonun İyi̇leşti̇ri̇lmesi̇: Olgu Sunumu

Stealth nanotubes: strategies of shielding carbon nanotubes to evade opsonization and improve biodistribution

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