Cytotoxicity of Ear Drop Excipients in Human and Mouse Tympanic Membrane Fibroblasts

Dirain, Carolyn O.; Karnani, David N; Antonelli, Patrick J.

doi:10.1177/0194599819889701

Cited by 6 publications

(5 citation statements)

References 28 publications

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“…DMSO, as a common cryoprotectant in practical applications, was used to compare with peptoids in a cytotoxicity test. Generally speaking, samples with a cell survival rate of less than 75% are considered to be cytotoxic [36]. The results show that the relative cell viability of all peptoid oligomer solutions was greater than 75% at a concentration of 10 mg/mL, which is considered to be non-cytotoxic (Figure 4).…”

Section: Sample Namementioning

confidence: 98%

Preparation of Peptoid Antifreeze Agents and Their Structure–Property Relationship

Yang,

Liu,

Feng

et al. 2024

Polymers

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The development of nontoxic and efficient antifreeze agents for organ cryopreservation is crucial. However, the research remains highly challenging. In this study, we designed and synthesized a series of peptoid oligomers using the solid-phase submonomer synthesis method by mimicking the amphiphilic structures of antifreeze proteins (AFPs). The obtained peptoid oligomers showed excellent antifreeze properties, reducing the ice crystal growth rate and inhibiting ice recrystallization. The effects of the hydrophobicity and sequence of the peptoid side chains were also studied to reveal the structure–property relationship. The prepared peptoid oligomers were detected as non-cytotoxic and considered to be useful in the biological field. We hope that the peptoid oligomers presented in this study can provide effective strategies for the design of biological cryoprotectants for organ preservation in the future.

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Section: Sample Namementioning

confidence: 98%

Preparation of Peptoid Antifreeze Agents and Their Structure–Property Relationship

Yang,

Liu,

Feng

et al. 2024

Polymers

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“…Upon matching, the types of medications taken by participants were primarily focused on treating conditions such as diabetes, hypertension, infections, and hyperlipidemia, among others. As detailed information regarding participants' medication, including dosage, duration, administration method, and brand was unavailable, we relied on clinical drug experience and existing literature evidence to primarily define participants taking the following 11 drugs (acetaminophen [21], hydrocodone [22], ciprofloxacin [23], phenytoin [24], levofloxacin [25], rifampin [26], minocycline [27], aspirin [28], metronidazole [29], nitroglycerin [30], and bumetanide [31]) as a high-risk group for drug-induced hearing loss and subsequently excluded them from the study (n = 46).…”

Section: Study Populationmentioning

confidence: 99%

Association between blood volatile organic aromatic compound concentrations and hearing loss in US adults

Wang,

Luo,

Zhang

et al. 2024

BMC Public Health

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Objective Benzene, ethylbenzene, meta/para-xylene, and ortho-xylene, collectively referred to as benzene, ethylbenzene, and xylene (BEX), constitute the main components of volatile organic aromatic compounds (VOACs) and can have adverse effects on human health. The relationship between exposure to BEX and hearing loss (HL) in the adult U.S. population was aimed to be assessed. Methods Cross-sectional data from the National Health and Nutrition Examination Survey (NHANES) for the years 2003–2004, 2011–2012, and 2015–2016 were analyzed. This dataset included complete demographic characteristics, pure-tone audiometry measurements, and volatile organic compound detection data from the NHANES database. A weighted multivariate logistic regression model was employed to investigate the associations between blood BEX concentrations HL, low-frequency hearing loss (SFHL), and high-frequency hearing loss (HFHL). Results 2174 participants were included, with weighted prevalence rates of HL, SFHL, and HFHL being 46.81%, 25.23%, and 45.86%, respectively. Exposure to benzene, ethylbenzene, meta/para-xylene, and ortho-xylene, and cumulative BEX concentrations increased the risk of hearing loss (odds ratios [ORs] were 1.36, 1.22, 1.42, 1.23, and 1.31, respectively; all P < 0.05). In the analysis with SFHL as the outcome, ethylbenzene, m-/p-xylene, o-xylene, benzene, and overall BEX increased the risk (OR 1.26, 1.21, 1.28, 1.20, and 1.25, respectively; all P < 0.05). For HFHL, exposure to ethylbenzene, m-/p-xylene, o-xylene, benzene, and overall BEX increased the risk (OR 1.36, 1.22, 1.42, 1.22, and 1.31, respectively; all P < 0.05). Conclusion Our study indicated that a positive correlation between individual or cumulative exposure to benzene, ethylbenzene, meta/para-xylene, and ortho-xylene and the risk of HL, SFHL, and HFHL. Further research is imperative to acquire a more comprehensive understanding of the mechanisms by which organic compounds, notably BEX, in causing hearing loss and to validate these findings in longitudinal environmental studies.

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“…201802084). The procedure for establishing the human cell line has been previously described (3,21).…”

Section: Cell Culture and Treatmentmentioning

confidence: 99%

Cytotoxicity of Tetracyclines in Human Tympanic Membrane Fibroblasts

Dirain

Antonelli

2023

Otol Neurotol

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Hypothesis: Tetracyclines are less cytotoxic to tympanic membrane (TM) fibroblasts than quinolones. Background: Use of quinolone ear drops after tympanostomy tube placement and for acute otitis externa has been linked to an increased risk of TM perforation. This has been verified in animal models. Cell culture studies have shown quinolones to be highly toxic to TM fibroblasts. Tetracyclines are a potential alternative to quinolones as they have been used to treat acute otitis externa and are thought to be nontoxic to the inner ear. We aimed to determine if tetracyclines are cytotoxic to TM fibroblasts. Methods: Human TM fibroblasts were treated with 1:10 dilutions of ofloxacin 0.3%, ciprofloxacin 0.3%, doxycycline 0.3 and 0.5%, minocycline 0.3 and 0.5%, tetracycline 0.3 and 0.5%, or dilute HCl (control), twice within 24 hours or four times within 48 hours. After 2 hours of treatment, cells were returned to growth media. Cells were observed with phase-contrast microscopy until cytotoxicity was measured.Results: Fibroblasts had lower survival with ciprofloxacin 0.3% and doxycycline 0.5% treatment compared with the control after 24 and 48 hours (all p < 0.0001). Fibroblasts treated with minocycline 0.5% had increased cell survival after 24 hours. Minocycline 0.3 and 0.5% showed increased TM fibroblast survival after 48 hours (all p < 0.0001). Phase-contrast images mirrored the cytotoxicity findings. Conclusions: Tetracyclines are less toxic to cultured TM fibroblasts than ciprofloxacin. Fibroblast tetracycline toxicity is drug and dose specific. Minocycline shows the most promise for possible otic applications in which fibroblast toxicity is a concern.

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Cytotoxicity of Ear Drop Excipients in Human and Mouse Tympanic Membrane Fibroblasts

Cited by 6 publications

References 28 publications

Preparation of Peptoid Antifreeze Agents and Their Structure–Property Relationship

Preparation of Peptoid Antifreeze Agents and Their Structure–Property Relationship

Association between blood volatile organic aromatic compound concentrations and hearing loss in US adults

Cytotoxicity of Tetracyclines in Human Tympanic Membrane Fibroblasts

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