Cytotoxicity of fumonisin B 1 : implication of lipid peroxidation and inhibition of protein and DNA syntheses

Abstract

The effects of fumonisin B1 (FB1) from Fusarium moniliforme on lipid peroxidation and protein and DNA syntheses were studied in monkey kidney cells (Vero cells). FB1 was found to be a potent inducer of malondialdehyde (MDA), one of the secondary products formed during lipid peroxidation. At 0.14 microM (0.1 microg/ml), FB1 induced 0.496 +/- 0.1 nmoles of MDA/ mg protein, compared to the control level 0.134 +/- 0.01 nmoles of MDA/mg protein (P < 0.005). No inhibition of protein or DNA synthesis was observed at this concentration of FB1. Inhibition of protein and DNA syntheses was observed at FB1 concentrations > 14 microM (10 microg/ml) with an IC50 of 33 microM for both protein synthesis and DNA synthesis. These results indicate that lipid peroxidation is a very sensitive cellular response to the mycotoxin fumonisin B1 observed at concentrations lower than that required to inhibit cellular synthesis of macromolecules, protein and DNA. This oxidative damage induced by FB1 concentrations encountered in naturally contaminated foodstuffs and feed might lead to mutagenicity and genotoxicity.

Feed exposure to FB1 can aggravate pneumonic damages in pigs provoked by P. multocid a

“…FB 1 has been reported to be a nephrotoxic for kidney (Bucci et al, 1998;Voss et al, 2001;Howard et al, 2001) and to provoke in vitro and in vivo degenerative and/or apoptotic damages in tubular epithelium of kidneys in rats or pigs (Dragan et al, 2001, Stoev et al, 2012, which was confirmed by the present study. FB 1 is also reported to impair protein synthesis in cells, which could lead to further degenerative changes in kidneys or liver, the target organs responsible for elimination or detoxification of mycotoxins (Abado-Becognee et al, 1998).…”

UV-vis spectroscopic study of lipid peroxidation and antioxidant contents of kidney of Albino mice co-administrated with sildenafil citrate and alcohol

“…Increases in lipid peroxidation leads to cell damage. According to Karine et al [1], once lipid peroxidation is initiated, it can easily be propagated in the cellular environment where unsaturated lipids and oxygen are present. The oxidation of lipids is propagated by radical mediated chain reactions.…”

Mycotoxins Affecting the Kidney

“…Two further theories have been proposed to explain the mechanism of action of FB 1 : disruption of fatty acid metabolism and the induction of oxidative stress, and modulation of gene expression (Abado-Becongnee et al, 1998;Mobio et al, 2000). In contrast to the sphingolipid imbalance theory, which can account for all of the known effects of FB 1 either in vivo or in vitro, these alternative theories cannot.…”