Cytotoxicity of functionalized carbon nanotubes in J774A macrophages

Suitability of the Composite Made of Multi Wall Carbon Nanotubes-Polyvinylpyrrolidone for Culturing Invertebrate <i>Helix aspersa</i> Neurons

“…Moreover, the results presented here corroborate the results reported by others [7] [8] [10], because in these experiments MWCNTs interact with living cells and act as functional scaf folds for neuronal tissue. An interesting finding reported here, is that it was no necessary to label the MWCNTs with fluorescent compounds, as it has been done and has been reported by others [5] [16] [17] [18] [19]. These MWCNTs were well identified with confocal microscopy studies (see Figure 3 and Figure 5) when the microscope was used in the reflection mode.…”

“…Kim et al [4] reported that multi wall carbon nanotubes induced DNA damage and cytotoxicity in male's human peripheral blood lymphocytes. Montes-Fonseca et al [5] found cytotoxicity of functionalized carbon nanotubes in J774A macrophages. Sachar et al [6] reported a cytotoxic effect of single wall carbon nanotubes (SWCNTs) on erythrocytes in vitro and in vivo.…”

Functionalized carbon nanotubes: biomedical applications

“…17 Similarly, enhanced interaction times resulted in higher amounts of apoptosis in both fCNTs and CNTs, though fCNTs were less apoptotic. 34 Another study involving HeLa cells treated with increased doses of functionalized SWCNTs and MWCNTs showed a 50% reduction in cell number. 35 In a similar study, human epidermal keratinocytes when treated with 0.00000005-0.05 mg/mL of 6-aminohexanoic acid-derivatized (AHA)-SWCNTs resulted in diminished cell viability and escalation in the expression of cytokines, demonstrating that greater concentrations of AHA-SWCNTs were cytotoxic.…”

“…Moreover, the expression of cytotoxic behavior is a measure of the purification process as well as the functional groups attached to the CNTs, thereby enabling them to establish cross talk with the cell-surface receptors. 34 CNTs coated with mucin-like polymers were able to interact with the carbohydrate receptors on the cell surface, offering them a way to interact with the cell surface minus any toxic effect. 66 Glucosamine-functionalized SWCNTs were able to improve the interactions of the cells with SWCNTs.…”

Compósito De Nanocelulose Bacteriana E Nanotubos De Carbono

“…(Park et al, 2009;Yan et al, 2008) A viabilidade celular, por exemplo, está relacionada à atividade mitocondrial das células e indica, caso estudada em um biomaterial, a citotoxicidade do mesmo, podendo ser avaliada por diferentes métodos como a técnica fotocolorimétrica utilizando-se o reagente MTT. O MTT é um sal de tetrazólio que reduzido a um derivado formazan de cor roxa, devido à atividade oxidativa, indica a existência de função mitocondrial e, por conseguinte, a viabilidade celular (Montes-Fonseca et al, 2012). …”