Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrations

Abstract

Stem cells from human exfoliated deciduous teeth (SHED) can be used as a cell-based therapy in regenerative medicine and in immunomodulation. Pulp from human deciduous teeth can be stored as a source of SHED. Glass ionomer cements (GICs) are commonly used in restorative dentistry and in cavity lining. GICs have lower biocompatibility and are cytotoxic for dental pulp cells. In this study, seven commonly used GICs were tested for their cytotoxic effects on SHED, for their potential to arrest mitosis in cells and induce chromosome aberrations, and were compared with the effects of composite. Fuji II, Fuji VIII, Fuji IX, Fuji plus and Vitrebond had significantly higher cytotoxic effects on SHED than composite. Only SHEDs that have been treated with Fuji I, Fuji IX, Fuji plus and composite recovered the potential for proliferation, but no chromosome aberrations were found after treatment with GICs. The cytotoxic effects of GICs on SHEDs were in strong correlation with combined concentrations of released fluoride, aluminum and strontium ions. Fuji I exhibited the lowest activity towards SHEDs; it did not interrupt mitosis and did not induce chromosome aberrations, and was accompanied by the lowest levels of released F, Al and Sr ions. Projekat Ministarstva nauke Republike Srbije, br. ON175069, br. ON175071 i br. ON175103]

Reformulated mineral trioxide aggregate components and the assessments for use as future dental regenerative cements

“…After 24 h of co-culturing, a significant increase in cell viability (~120%) was observed in all groups except group 13 (C3A) compared to the media control, ( Figure 3 , p < 0.05) The aluminum containing C3A cement indicated the lowest cell viability (~60%, p < 0.05), while the other cements including even 20% C3A (G8, G9, and G10) did not exhibit cytotoxicity, and instead revealed increased cell viability. Aluminum ion is well-known to induce cytotoxicity against mammalian cells due to its destruction of lipid layer and DNA synthesis, 34 , 35 thus it is considered that the released aluminum ions from C3A (100% C3A) may be the major cause of C3A-induced cytotoxicity. In fact, other toxic metallic elements (e.g.…”

The effect of surface pre‐reacted glass‐ionomer filler eluate on dental pulp cells and mineral deposition on dentin: In vitro study

“…Meanwhile, there are various reports on the toxicity of these ions, especially F [34]. Flouride ions released from glass ionomer cement (GIC) have been reported to inhibit cell growth and matrix synthesis in a dose-dependent manner [4,35,36], and thus, the cytotoxicity of GIC on human dental pulp stem cells correlates with the amount of released F [34,37]. Al ions inhibit collagen formation and negatively affect the mineralization of cells [38], and Sr ions interfere with the ALP activity and nodule formation of osteoblast cells [39].…”

Evaluation of the Possible Neurotoxic Effect of the Bone Cement on the Facial Nerve: An Experimental Study

“…Since the use of BC is becoming more widespread researches about BC is increasing gradually. Studies have shown that BC has cytotoxic effects on odontoblasts, milk teeth stem cells, mouse lymphoma cells, macrophages, fibroblasts, mouse embryonic stem cells, and dental pulp cells [ 6 , 16 - 21 ]. In literature, despite the reports of various side effects of BC on neurons, and neurotoxicity cases resulted in death, objective and evidence-based studies towards BC-related neurotoxicity are still inadequate.…”