Cytotoxicity of GuttaFlow Bioseal, GuttaFlow2, MTA Fillapex, and AH Plus on Human Periodontal Ligament Stem Cells

Collado-González, Mar; Tomás-Catalá, Christopher J.; Sánchez, Ricardo Elías Oñate; Moraleda, José Marı́a; Rodríguez‐Lozano, Francisco Javier

doi:10.1016/j.joen.2017.01.001

Cited by 90 publications

(97 citation statements)

References 34 publications

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“…Since calcium oxide in contact with water give rise to calcium hydroxide and, consequently, favors the nucleation of hydroxyapatite deposits 8 , it has been suggested that GFB is bioactive and, therefore, stimulates the repair of mineralized tissues 8 . Although, an in vitro study reported low cytotoxicity of the GFB on human periodontal fibroblasts 10 , in vivo studies evaluating the tissue reaction provoked by this new silicone-based sealer were not found. The present results revealed that the moderate inflammatory infiltrate elicited by GFB was transitory since the number of inflammatory cells, inflammatory reaction score and the immunoexpression of IL-6 decreased over time.…”

Section: Discussionmentioning

confidence: 94%

Immunoinflammatory response and bioactive potential of GuttaFlow bioseal and MTA Fillapex in the rat subcutaneous tissue

Delfino

Guerreiro-Tanomaru

Tanomaru‐Filho

et al. 2020

Sci Rep

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To evaluate the effect of GuttaFlow bioseal (GFB) and MTA Fillapex (MTAF) in comparison with Endofill (EF) in the subcutaneous tissue. Polyethylene tubes with GFB, MTAF, EF or empty tubes (control group; CG) were implanted into subcutaneous of rats. After 7, 15, 30 and 60 days, the capsule thickness, inflammatory reaction, interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), caspase-3, TUNEL-positive cells, von Kossa and ultrastructural features were evaluated. The data were statistically analyzed (p ≤ 0.05). At all periods, the number of IL-6-and VEGF-immunolabelled cells, and capsule thickness were lower in GFB than MTAF, which was lower than EF (p < 0.0001). At 60 days, the number of inflammatory cells was similar in GFB and MTAF (p = 0.58). Significant differences in the number of TUNEL-and caspase-3-positive cells were not observed among GFB, MTAF and CG whereas the highest values were found in EF specimens. The EF specimens exhibited several cells with condensed chromatin, typical of apoptosis. von Kossa-positive and birefringent structures were only observed in GFB and MTAF, suggesting the presence of calcite crystals. Taken together, these results show that cellular and structural damage induced by GFB and MTAF sealers were recovery over time. Moreover, these sealers express bioactive potential in subcutaneous tissue.The clinical use indication of root canal sealers depends on their physicochemical and biological properties. Thus, the biological properties of endodontic sealers and degree of cytotoxicity on different cells, including fibroblasts, osteoblasts and cementoblasts have been widely investigated 1 . However, in vitro studies have the disadvantage of reflecting the response to a specific cell type and, therefore, do not reflect the host response, which is under influence of several cytokines and growth factors 2 . The implant into subcutaneous connective tissue is widely used to evaluate the biocompatibility and the complex cascade of cellular and molecular events induced by dental materials 2-5 .Studies have suggested that silicone-based endodontic sealers exhibit proper physicochemical and biological properties 6,7 . A novel formulation of silicone-based sealer, the GuttaFlow bioseal (GFB; Coltene Whaledent, GmBH + Co KG, Langenau, Switzerland) is a mixture of gutta-percha powder and polydimethylsiloxane with silver nanoparticles added as a preservative; silica, calcium oxide and phosphorous oxide particles were also combined to this mixture to provide bioactivity and stimulate the tissue repair. The GFB has low solubility and porosity, alkalinizing activity and slight calcium release 8,9 . An in vitro study using human periodontal ligament cells has demonstrated that GFB displays better cytocompatibility than AH Plus 10 , which is considered as a gold standard in the clinical endodontic.Considering the suitable biocompatibility of MTA (Mineral Trioxide Aggregate, Angelus, Londrina, PR, Brazil), the manufactures have added tricalcium silicate to resin-based sealers in an attempt to ...

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Section: Discussionmentioning

confidence: 94%

Immunoinflammatory response and bioactive potential of GuttaFlow bioseal and MTA Fillapex in the rat subcutaneous tissue

Delfino

Guerreiro-Tanomaru

Tanomaru‐Filho

et al. 2020

Sci Rep

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“…Multipotent hPDLSCs were isolated from human periodontal ligament tissue (n = 5) as described previously (Collado-Gonzalez et al 2017). The study was approved by the Institutional Ethics Committee (Virgen de la Arrixaca University Hospital ID: 2017-3-8-HCUVA), whilst all patients signed an informed consent form.…”

Section: Isolation and Culture Of Hpdlscsmentioning

confidence: 99%

“…Finally, the absorbance at 570 nm (Abs 570 ) was measured with an automatic microplate reader (ELx800; Bio-Tek Instruments, Winooski, VT, USA) using Abs 690 as the reference wavelength. The MTT assay was performed in three independent experiments, with three replicate wells for each experimental point (Tomas-Catala et al 2017).…”

Section: Mtt Assaymentioning

confidence: 99%

“…Recently, new bioactive materials have been introduced (Farrugia et al 2018, Pedano et al 2018, amongst them, NeoMTA-Plus, a hydraulic tricalcium silicate-based that contains tantalum oxide as a radiopacifying agent in place of bismuth oxide, thus avoiding discoloration and can enhance mineralization potential by inducing differentiation into mineralsecreting cells (Tomas-Catala et al 2017. Furthermore, NeoMTA-Plus has been reported to have a capacity to release calcium, prevent bacterial leakage, adequate radiopacity and a satisfactory sealing ability; it can be used as endodontic sealer or cement repair (Quintana et al 2018).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of changes in ion release and biological properties of NeoMTA‐Plus and Endocem‐MTA exposed to an acidic environment

et al. 2019

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Aim To analyse in vitro changes in ion release and biological properties of Endocem‐MTA (Maruchi, Wonju, Korea) and NeoMTA‐Plus (Avalon Biomed Inc, Bradenton, FL, USA) exposed to acidic or neutral environment on human dental periodontal ligament stem cells (hPDLSCs). Methodology Cell viability and wound healing assays were performed using eluates of each material. Cell death and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. To evaluate cell attachment to the different materials, hPDLSCs were directly seeded onto the material surfaces and analysed by scanning electron microscopy. The chemical composition of the materials was determined by energy‐dispersive X‐ray (EDX), and ion release was evaluated by inductively coupled plasma‐mass spectrometry. Statistical analysis was performed with analysis of variance and a Bonferroni or Tukey post‐test (α < 0.05). Results The MTT assay revealed non‐cytotoxic effects of NeoMTA‐Plus and Endocem‐MTA at pH 5.2 and 7.4. However, there were minor differences compared with the control, especially at pH 5.2, where both materials were associated with significantly greater cell viability (P < 0.05). In both environments, the materials stimulated hPDLSCs to migrate. hPDLSCs were attached to the bioactive cements, with multiple prolongations proliferated on the surface of the samples. Moreover, there were no changes to cell phenotype or apoptosis/necrosis rates, indicating that the acidic environment did not induce cell death. Prismatic crystalline structures were seen on the surface of the cements exposed to butyric acid and EDX analysis identified a marked peak of Ca2+ from NeoMTA‐Plus and Endocem‐MTA in acidic and physiological environments. Conclusions An acidic environment favoured the release of Ca2+ ions from both bioactive cements, and the cytotoxicity of these bioactive cements was low in both environments studied.

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“…Dental sealers were weighted under sterile conditions as 0.5gm, and stored in an incubator at 37 0 C for 24 hours to achieve complete setting. Hanks Balanced salt solution (HBSS) as simulating body fluid (Thermo Fisher Scientific, USA) was added to the set sealer for 7 days, to simulate the clinical situation whereby endodontic sealer is present in the apical region of the root and extruded over the apex, a region where body fluids are found (21) . The eluates of the different materials were extracted in sterile conditions by using Dulbecco modified Eagle medium (DMEM) culture medium as extraction vehicle.…”

Section: Sealer Extractsmentioning

confidence: 99%

Cytotoxicity of Three Root Canal Sealers on Human Fibroblast Cells (Wi-38) at Different Times and Concentrations

Beshr¹,

Abdelrahim²

2018

Egyptian Dental Journal

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Introduction:The aim of the present study was to evaluate the in vitro cytotoxicity of three endodontic sealers (TotalFill BC, Gutta Flow2 and AH Plus) on WI-38 cell line human. As a reference, AH Plus was compared with the more recent endodontic sealers regarding cell viability of fibroblast cells (WI-38). Methods:Biological testing was carried out in vitro on WI-38 cells. Cell viability assay was performed by using eluates from each endodontic sealer and dispensed to pre-cultured WI-38 cell line. The effect was assessed by MTT cell proliferation assay and ELISA reader after 24 hours and 72 hours of exposure and various concentrations of (0.5, 1,2,4,8,16,32, 64, 125, 250 and 500mg/ ml). Statistical differences were assessed by analysis of variance and Tukey test (P < .05). Results:TotalFill BC, Gutta Flow2 and AH Plus sealer eluates for 24 hours showed no cytotoxic effect, while it was increased by increasing the concentration for 72 hours' measurement. Statistically there is no significant difference among the tested sealer materials; TotalFill BC, Gutta Flow2 and AH Plus. Conclusions:In the present study TotalFill BC, Gutta Flow2 and AH Plus sealer eluates showed no cytotoxic effects at least in the first 24 hours. But they exhibited moderately or severely cytotoxic activity at 72 hours of measurement at high concentration.

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Cytotoxicity of GuttaFlow Bioseal, GuttaFlow2, MTA Fillapex, and AH Plus on Human Periodontal Ligament Stem Cells

Cited by 90 publications

References 34 publications

Immunoinflammatory response and bioactive potential of GuttaFlow bioseal and MTA Fillapex in the rat subcutaneous tissue

Immunoinflammatory response and bioactive potential of GuttaFlow bioseal and MTA Fillapex in the rat subcutaneous tissue

Evaluation of changes in ion release and biological properties of NeoMTA‐Plus and Endocem‐MTA exposed to an acidic environment

Cytotoxicity of Three Root Canal Sealers on Human Fibroblast Cells (Wi-38) at Different Times and Concentrations

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