Cytotoxicity of <i>Porphyromonas gingivalis</i> toward cultured human gingival fibroblasts

Morioka, Masami; Hinode, Daisuke; Nagata, Atsushi; Hayashi, Hiroyuki; Ichimiya, Seiko; Ueda, Mari; Kido, Reiko; Nakamura, Ryô

doi:10.1111/j.1399-302x.1993.tb00560.x

Cited by 29 publications

(14 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We used two in vitro models of P. gingivalis – host cell interaction. First, we used a cellular cytotoxicity assay (22, 25) where P. gingivalis (strain W83) was added to confluent cell cultures resulting in morphological changes in both the oral epithelial cell line OKF6/TERT-2 and in HGEC. These changes included loss of adhesion, cell rounding, and detachment (Fig.…”

Section: Resultsmentioning

confidence: 99%

Identification of interspecies interactions affectingPorphyromonas gingivalisvirulence phenotypes

Tenorio

Klein

Cheung

et al. 2011

Journal of Oral Microbiology

View full text Add to dashboard Cite

BackgroundPeriodontitis is recognized as a complex polymicrobial disease, however, the impact of the bacterial interactions among the 700–1,000 different species of the oral microbiota remains poorly understood. We conducted an in vitro screen for oral bacteria that mitigate selected virulence phenotypes of the important periodontal pathogen, Porphyromonas gingivalis.MethodWe isolated and identified oral anaerobic bacteria from subgingival plaque of dental patients. When cocultured with P. gingivalis W83, specific isolates reduced the cytopathogenic effects of P. gingivalis on oral epithelial cells.ResultIn an initial screen of 103 subgingival isolates, we identified 19 distinct strains from nine species of bacteria (including Actinomyces naeslundii, Streptococcus oralis, Streptococcus mitis, and Veilonella dispar) that protect oral epithelial cells from P. gingivalis-induced cytotoxicity. We found that some of these strains inhibited P. gingivalis growth in plate assays through the production of organic acids, whereas some decreased the gingipain activity of P. gingivalis in coculture or mixing experiments.ConclusionIn summary, we identified 19 strains isolated from human subgingival plaque that interacted with P. gingivalis, resulting in mitigation of its cytotoxicity to oral epithelial cells, inhibition of growth, and/or reduction of gingipain activity. Understanding the mechanisms of interaction between bacteria in the oral microbial community may lead to the development of new probiotic agents and new strategies for interrupting the development of periodontal disease.

show abstract

Section: Resultsmentioning

confidence: 99%

Identification of interspecies interactions affectingPorphyromonas gingivalisvirulence phenotypes

Tenorio

Klein

Cheung

et al. 2011

Journal of Oral Microbiology

View full text Add to dashboard Cite

show abstract

“…HGFs were obtained from a gingival biopsy of a 20-year-old person with clinically healthy periodontium and maintained according to the method of Morioka et al [9]. The cells from passages 10^14 were used for the following experiments.…”

Section: Preparation Of Hgf Measurement Of Il-6 and Il-8 Secretion mentioning

confidence: 99%

The GroEL-like protein from Campylobacter rectus: immunological characterization and interleukin-6 and -8 induction in human gingival fibroblast

Hinode

1998

FEMS Microbiology Letters

View full text Add to dashboard Cite

The native GroEL-like protein was purified from Campylobacter rectus, a putative periodontal pathogen, by affinity chromatography on ATP-agarose followed by high performance liquid chromatography on Superose 6. The purified 64-kDa protein (denatured form of GroEL-like protein) was immunoreactive by SDS-PAGE and Western immunoblotting with the monoclonal antibody directed against heat shock protein 60 of human origin. The native GroEL-like protein stimulated both interleukin-6 (IL-6) and IL-8 secretion by a confluent monolayer of human gingival fibroblast in their culture supernatant. During the 22-h incubation, the amounts of IL-6 and IL-8 were increased by 5.4-and 3.5-fold, respectively. These data suggested that the GroEL-like protein might be considered to be a virulence factor of C. rectus in periodontal disease. z 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

show abstract

“…35 In this study we demonstrated that proliferation of human PDL cells was reduced significantly by the treatment of the cells with a P. gingivalis extract, as observed in gingival fibroblasts. [26][27] The highest concentration of bacterial extract (100 pg/mL) inhibited cell proliferation completely, caused cell death, and altered cell morphology. The presence of P. gingivalis at higher concentrations may directly destroy the periodontal ligament.…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial components, such as surface-associated material, outer membrane, outer membrane vesicles, and fimbriae from outer membrane were able to cause either growth inhibition or cytotoxicity in fibroblasts. [25][26] Our bacterial extract contained all these factors, except for outer membrane vesicles which are normally obtained from the supernatant of the culture. Proteolytic enzymes, such as collagenases and aminopeptidases, have long been considered pathogens since periodontal tissues consist mostly of collagen, which is a substrate for these enzymes.26-28 If proteolytic enzymes in our P. gingivalis extract changed cell surface molecules, degradation of PDGF-R in the membrane may occur.…”

Section: Discussionmentioning

confidence: 99%

“…23 24 On the other hand, direct destructive effects such as cytotoxicity and membrane degradation of P. gingivalis-derìved virulence factors on cultured gingival fibroblasts have also been reported. [25][26][27][28] Recently, Bartold et al. showed that PDGF reduced the inhibitory effect of LPS derived from Salmonella on the proliferation of human gingival fibroblasts,29 suggesting that a balance between bacterial factors and growth factors is critical in periodontal tissue during regeneration as well as its destruction.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Porphyromonas gingivalis Reduces Mitogenic and Chemotactic Responses of Human Periodontal Ligament Cells to Platelet‐Derived Growth Factor In Vitro

Matsuda¹,

Takemura

Taniguchi

et al. 1996

Journal of Periodontology

View full text Add to dashboard Cite

The effects of a sonicated Porphyromonas gingivalis ATCC 33277 protein extract on the mitogenic and chemotactic responses of human periodontal ligament (PDL) cells to the recombinant human platelet‐derived growth factor‐BB homodimer (PDGFBB) were examined in vitro. Proliferation of PDL cells was inhibited by P. gingivalis extract at concentrations higher than 10 μg/mL protein. At 100 μg/mL of P. gingivalis extract, cells did not proliferate. DNA synthesis in PDL cells, as revealed by [3H]thymidine incorporation, was also inhibited by approximately 50% in the presence of 50 μg/mL P. gingivalis extract for 24 hours. In contrast, PDGF‐BB at 1 ng/mL enhanced DNA synthesis in PDL cells, followed by maximum enhancement at concentrations higher than 10 ng/mL PDGF‐BB. However, this mitogenic response to PDGF‐BB was markedly reduced in the presence of 20 μg/mL of P. gingivalis extract and did not reach the maximum level even if PDGF‐BB concentrations were increased to 250 ng/mL. PDL cells exhibited a chemotactic response to PDGF‐BB at 1 ng/mL, which was also inhibited by pretreatment of the cells with P. gingivalis extract at 10 to 50 pg/mL. Scatchard analysis of a [125I]‐PDGF binding assay demonstrated that PDL cells have both high and low PDGF binding affinity sites. Treatment of the cells with P. gingivalis extract decreased the number of PDGF‐binding sites to approximately 35% of the control level, while it caused only a slight change in the affinities of both types of binding site. These results indicated that the P. gingivalis extract reduced mitogenic and chemotactic responses of human PDL cells, possibly through mechanisms involving a decrease in PDGF‐binding capacity of these cells. Due to this inhibitory effect of P. gingivalis, the normal levels of PDGF in periodontal lesions may not be sufficient to promote periodontal regeneration through activation of PDL cell proliferation and migration. Therefore, the therapeutic use of PDGF‐BB, as a supplement to pre‐existing PDGF and as an adjunct, while also eliminating P. gingivalis from periodontal lesions, would help periodontal tissue regeneration. J Periodontol 1996;67:1335–1341.

show abstract

Cytotoxicity of Porphyromonas gingivalis toward cultured human gingival fibroblasts

Cited by 29 publications

References 25 publications

Identification of interspecies interactions affectingPorphyromonas gingivalisvirulence phenotypes

Identification of interspecies interactions affectingPorphyromonas gingivalisvirulence phenotypes

The GroEL-like protein from Campylobacter rectus: immunological characterization and interleukin-6 and -8 induction in human gingival fibroblast

Porphyromonas gingivalis Reduces Mitogenic and Chemotactic Responses of Human Periodontal Ligament Cells to Platelet‐Derived Growth Factor In Vitro

Contact Info

Product

Resources

About