Cytotoxicity of Metabolites Produced by Endophytic Fungus Cladosporium Sp. Isolated From Marine Macroalgae on In­vitro McF­7, Hela, and Du-145 Cell Lines

Abstract

Objective: To investigate the in vitro cytotoxicity effect of the crude ethyl acetate extract of Cladosporium sp. on MCF-7, HeLa, and DU-145 cell lines.Methods: In vitro cytotoxicity was evaluated by tetrazolium reduction assay. The percentage of cell inhibition was analyzed using probit analysis to obtain 50% inhibitory concentration (IC50). Morphological alteration of the cell lines after exposure with extract was observed under an inverted microscope.Results: The ethyl acetate extract of the metabolite performed an anticancer activity for cancer cell line MCF-7, HeLa, and DU-145 with IC50 respectively 8.46 μg/ml; 9.87 μg/ml; and 98.03 μg/ml. The extract shows greater the anticancer activity and has strong antiproliferative on MCF-7 and HeLa cell line than DU-145. Confirmation morphological were observed under the inverted microscope showed a morphological change in cancer cells when incubated with the extract.Conclusion: From the performed assay, the crude extract of Cladosporium sp. exhibit cytotoxic activity against MCF-7, HeLA, and DU-145.

Eco-Sustainable Approaches to Prevent and/or Eradicate Fungal Biodeterioration on Easel Painting

“…and Fusarium sp. produce anhydrofusarubin-red [61,62]-or A. versicolor produces asperversin-yellow [63]. Red-orange, β-carotene, is the most produced pigment by Mucoromycota as a preventive measure for stress oxidative damages [64].…”

Cytotoxicity of Aromatic Compound From an Endophytic Fungus, Cladosporium Sp. En-S01

“…Breast cancer cell cytotoxicity testing was carried out on MCF-7 cell lines because these cell lines are very stable and can represent most invasive breast cancer cells. Wulandari et al (2018) proved that the crude extract with fermented ethyl acetate solvent from Cladosporium sp. EN-S01 shows the potential for cytotoxic activity against MCF-7 breast cancer cell lines with IC50 values of 8.46 μg/ml in very active category [3].…”

“…Wulandari et al (2018) proved that the crude extract with fermented ethyl acetate solvent from Cladosporium sp. EN-S01 shows the potential for cytotoxic activity against MCF-7 breast cancer cell lines with IC50 values of 8.46 μg/ml in very active category [3]. Important stages in the process of searching for new drugs (new drug discovery) in the fields of medical biotechnology and molecular biology are the discovery of drug molecules derived from plants, animals, and microorganisms by isolating and identifying the structure of pure compounds.…”