Cytotoxicity of methanol extracts of 10 Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines

Kuete, Victor; Tchinda, Cedric F.; Mambe, Flora T.; Beng, Véronique Penlap; Efferth, Thomas

doi:10.1186/s12906-016-1253-3

Cited by 44 publications

(32 citation statements)

References 51 publications

(70 reference statements)

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“…Accumulation of intracellular ROS was determined by flow cytometry using the Reactive Oxygen Species Assay Kit (Beyotime) as described previously . In brief, colon cancer cells were collected after treatment with DMC and/or 5‐Fu and resuspended in serum‐free medium containing 10 μ mol/L 2′, 7′‐dichlorodihydrofluorescein.…”

Section: Methodsmentioning

confidence: 99%

In vitro additive antitumor effects of dimethoxycurcumin and 5‐fluorouracil in colon cancer cells

Zhao

Liu²,

Wang

et al. 2017

Cancer Medicine

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Dimethoxycurcumin (DMC) is a lipophilic analog of curcumin, an effective treatment for colon cancer, which has greater chemical and metabolic stability. Chemotherapy treatments, such as 5‐fluorouracil (5‐Fu), play a key role in the current management of colon cancer. In this study, we investigated the antitumor efficacy of DMC in combination with 5‐Fu in SW480 and SW620 colon cancer cells. CCK‐8 assay was used to evaluate the inhibitory effect of DMC and 5‐Fu on cancer cells proliferation, and the combination index was calculated. The influence of DMC and 5‐Fu on cell cycle, apoptosis, reactive oxygen species (ROS) production, and mitochondrial membrane potential in SW480 and SW620 cells was determined using flow cytometry, and the related signaling pathways were detected by western blot. Transmission electron microscopy was used to observe endoplasmic reticulum expansion. DMC‐ and/or 5‐Fu‐induced apoptosis, stimulated G0/G1 phase arrest, increased ROS levels, decreased mitochondrial membrane potential, and enhanced endoplasmic reticulum expansion. The induction of apoptosis is involved in the increasing of Bax and cytochrome c and decreasing of Bcl2 expressions. Increased production of ROS was accompanied by upregulation of CHOP and Noxa. Combination therapy of DMC and 5‐Fu had increased efficacy on the above pathways compared with either drug alone. Based on the calculated IC 50, combination treatment with DMC and 5‐Fu had an additive antitumor effect in both cell lines. Combined treatment with DMC and 5‐Fu led to an additive antitumor effect in colon cancer cells that was related to apoptosis induction, G0/G1 phase arrest, increased ROS production, decreased mitochondrial membrane potential, and enhanced endoplasmic reticulum expansion.

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Section: Methodsmentioning

confidence: 99%

In vitro additive antitumor effects of dimethoxycurcumin and 5‐fluorouracil in colon cancer cells

Zhao

Liu²,

Wang

et al. 2017

Cancer Medicine

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“…The aqueous and methanolic extracts of A. amara exhibited cytotoxic effects to the MCF-7 breast cancer cells with IC 50 values of 83.16µg/ml and 57.53µg/ml, respectively. A stronger cytotoxicity was found in the ethyl acetate extract with an IC 50 value of 36.31µg/ml [41].…”

Section: Assessment Of Expression Of Apoptotic (P53) and Angiogenic (mentioning

confidence: 90%

Gene Expression Mediated Antiproliferative Potential and Safety of Selected Medicinal Plants Against Cancerous and Normal Cells

Mbugua

Njagi

Ngule

et al. 2019

Preprint

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Globally, approximately 13% of all deaths annually are attributed to cancer. Surgery, radiation and chemotherapy are the current treatment techniques for cancer, however these methods are expensive, have high failure rates and have been associated with detrimental side effects. Plant derived products could be good candidates in alleviating challenges being experienced with these current methods. This study aimed at evaluating the phytochemistry, antiproliferation potential, and probable mechanism of action of Albizia gummifera, Rhamnus staddo and Senna didymobotrya plant extracts. Phytochemical screening was done as per standard procedures. The common 3-(4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium (MTT) dye was used in the determination of the antiproliferative activity of the extracts. Extracts induction of VEGF (angiogenesis) and p53 (apoptosis) genes' expression was evaluated using Real Time Polymerase Chain Reaction. Phytochemical screening revealed presence of alkaloids, tannins, glycosides, flavonoids, terpenes, phenolics and saponins in the plants extracts. A. gummifera's stem bark methanol: dichloromethane extract had the highest activity against the cancerous cell lines tested: HCC1395 (IC 50 6.07±0.04µg/ml), DU145 (IC 50 3.34±0.05µg/ml), CT26 (IC 50 5.78±0.08µg/ml) and Hep2 (IC 50 7.02±0.01µg/ml). R. staddo root bark methanol: dichloromethane extract had an IC 50 value of 15.71±0.04µg/ml on HCC, 9.81±0.09µg/ml on Hep2 and 11.14±0.39µg/ml on CT26. S. didymobotrya root bark methanol: dichloromethane extract inhibited HCC with an IC 50 of 65.06±0.07µg/ml, CT26 with an IC 50 of 15.71±0.04µg/ml and Hep2 with an IC 50 of 62.10±0.11µg/ml. From the results obtained, the plants exhibited selective toxicity to cancer cells while sparing the normal cells (SI ≥ 3). A. gummifera and S. didymobotrya and R. staddo plant extracts upregulated p53 and down-regulated VEGF genes. In conclusion, this study confirms that these plant extracts could be potential candidates for development of drugs for the management of breast, prostrate, colorectal and throat cancer.

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“…Over the years, pharmacological studies on A. adianthifolia extracts and compounds extracted from the species showed potent in vitro and in vivo pharmacological activities including acetylcholinesterase enzyme inhibitory [ 69 , 72 , 73 ], anthelmintic [ 70 , 74 ], antiamoebic [ 74 ], antibacterial [ 27 , 53 , 68 , 73 , 75 ], antimycobacterial [ 76 ], anti-sexually transmitted infections [ 77 ], antifungal [ 27 , 68 ], anti-inflammatory [ 73 , 78 ], antioxidant [ 27 , 28 , 69 , 79 , 80 ], anxiolytic and antidepressant [ 79 ], cognitive-enhancing [ 28 ], haemolytic [ 66 , 81 ], hypoglycemic and antihyperglycemic [ 45 ], immunomodulatory [ 66 ], cytotoxicity [ 77 , 80 – 84 ].…”

Section: Pharmacological Activitiesmentioning

confidence: 99%

“…The cell viability assay indicated that the extracts were nontoxic at 100 mg/ml against the human kidney epithelial cell line, but 110% and 112% cell growth exhibited by aqueous and organic extracts, respectively, appeared to increase cellular activity, which would be effective in wound healing [ 77 ]. Kuete et al (2016) [ 84 ] evaluated the cytotoxicity activities of methanol bark and root extracts of A. adianthifolia against the sensitive leukemia CCRF-CEM cells. The extracts were further tested on a panel of eight human cancer cell lines, including MDR phenotypes.…”

Section: Cytotoxicity Activitiesmentioning

confidence: 99%

“…Both bark and root extracts were active against other cell lines and normal AML12 hepatocytes with IC 50 values ranging from 2.7 μ g/mL to 10.8 μ g/mL towards glioblastoma U87MG.ΔEGFR cells, breast adenocarcinoma MDA-MB-231-BCRP cells, and colon carcinoma HCT116(p53 −/− ) cells. The root extracts induced apoptosis in CCRF-CEM cells through caspases activation and loss of mitochondrial membrane potential [ 84 ]. Sulaiman et al (2017) [ 80 ] evaluated the cytotoxic activities of magnetic iron oxide nanoparticles synthesized using A. adianthifolia leaf extracts on human breast (AMJ-13) and (MCF-7) cancer cells.…”

Section: Cytotoxicity Activitiesmentioning

confidence: 99%

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Albizia Adianthifolia: Botany, Medicinal Uses, Phytochemistry, and Pharmacological Properties

Maroyi

2018

The Scientific World Journal

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The bark, leaves, and roots of Albizia adianthifolia are highly sought after in tropical Africa as herbal medicines. Therefore, the aim of this study was to review the botany, medicinal uses, phytochemistry, and pharmacological properties of A. adianthifolia so as to provide baseline data required for evaluating the therapeutic potential of the species. Information on the botanical profile, medicinal uses, phytochemistry, and pharmacological properties of A. adianthifolia was undertaken using databases such as ScienceDirect, SciFinder, Pubmed, Google Scholar, Medline, SCOPUS, EThOS, ProQuest, OATD, and Open-thesis. Preelectronic literature search of conference papers, scientific articles, books, book chapters, dissertations, and theses was carried out at the University library. Literature search revealed that A. adianthifolia is used as purgative and herbal medicine for diabetes, eye problems, gastrointestinal problems, haemorrhoids, headache, neurodegenerative disorders, reproductive problems in women, respiratory problems, wounds and pain, skin diseases, sexually transmitted infections, and ethnoveterinary medicine. Phytochemical compounds identified from the species include apocarotenoids, chalcone, dipeptide, elliptosides, essential oils, fatty acids, flavonoids, histamine, imidazolyl carboxylic acid, prosapogenins, steroids, triterpene saponins, and triterpenoids. Pharmacological studies revealed that A. adianthifolia extracts and compounds have acetylcholinesterase enzyme inhibitory, anthelmintic, antiamoebic, antibacterial, antimycobacterial, anti-sexually transmitted infections, antifungal, anti-inflammatory, antioxidant, anxiolytic, and antidepressant, cognitive-enhancing, haemolytic, hypoglycemic and antihyperglycemic, immunomodulatory, and cytotoxicity activities. Detailed studies on the pharmacokinetics, in vivo, and clinical research involving compounds isolated from A. adianthifolia and extracts of the species are required.

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Cytotoxicity of methanol extracts of 10 Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines

Cited by 44 publications

References 51 publications

In vitro additive antitumor effects of dimethoxycurcumin and 5‐fluorouracil in colon cancer cells

In vitro additive antitumor effects of dimethoxycurcumin and 5‐fluorouracil in colon cancer cells

Gene Expression Mediated Antiproliferative Potential and Safety of Selected Medicinal Plants Against Cancerous and Normal Cells

Albizia Adianthifolia: Botany, Medicinal Uses, Phytochemistry, and Pharmacological Properties

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