Cytotoxicity of nanosize V2O5 particles to selected fibroblast and tumor cells

Ivanković, Siniša; Musić, Svetozar; Gotić, Marijan; Ljubešić, Nikola

doi:10.1016/j.tiv.2005.08.011

Cited by 39 publications

(20 citation statements)

References 28 publications

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“…Some advantages of in vitro studies using various cell lines are that they: (1) reveal effects of target cells in the absence of secondary effects caused by inflammation; (2) permit the identification of primary mechanisms of toxicity in the absence of the physiological and compensatory factors that confound the interpretation of whole animal studies; and (3) are efficient, rapid and cost-effective; and 4) can be used to improve design of subsequent expensive whole animal studies. For instance, we and others have used A549 (human bronchoalveolar carcinoma-derived cells), U87 (astrocytoma cells), U937 (human monoblastoid cells), mouse Leydig TM3 cells, human V79 and L929 fibroblasts, human SCCVII, B16F10 and FsaR tumor cells, and RAW264.7 (mouse peritoneal macrophage) cell lines to characterize oxidative stress-related signaling pathways [32,33,34,35,36,37,38,39,40,41,42]. A nontransformed human lung cell line (BEAS-2B) has been used to decipher expression of alteration of genes pertaining to oxidative stress and cell death pathways [43,44].…”

Section: Methodologies To Study Toxicity Of Transition Metal Oxidesmentioning

confidence: 99%

“…Fahmy and Cormier also identified a similar relationship of CuO and Fe 2 O 3 toxicity in airway epithelial cells (HEp-2) [39]. It is difficult to compare the toxicity of TiO 2 and V 2 O 5 , as synthesis methods, particle characteristics, and cell lines (Leydig TM3 cells, human V79 and L929 fibroblasts, human SCCVII, B16F10 and FsaR tumor cells) differ among the few published studies; however, it is quite clear that TiO 2 is minimally toxic while V 2 O 5 is toxic [40,41,42]. Data from these studies are consistent with our findings presented in Figure 2.…”

Section: Toxicity Of Transition Metal Oxide Particlesmentioning

confidence: 99%

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Toxicity of Transition Metal Oxide Nanoparticles: Recent Insights from in vitro Studies

2010

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Nanotechnology has evolved to play a prominent role in our economy. Increased use of nanomaterials poses potential human health risk. It is therefore critical to understand the nature and origin of the toxicity imposed by nanomaterials (nanotoxicity). In this article we review the toxicity of the transition metal oxides in the 4th period that are widely used in industry and biotechnology. Nanoparticle toxicity is compellingly related to oxidative stress and alteration of calcium homeostasis, gene expression, pro-inflammatory responses, and cellular signaling events. The precise physicochemical properties that dictate the toxicity of nanoparticles have yet to be defined, but may include element-specific surface catalytic activity (e.g., metallic, semiconducting properties), nanoparticle uptake, or nanoparticle dissolution. These in vitro studies substantially advance our understanding in mechanisms of toxicity, which may lead to safer design of nanomaterials.

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Section: Methodologies To Study Toxicity Of Transition Metal Oxidesmentioning

confidence: 99%

Section: Toxicity Of Transition Metal Oxide Particlesmentioning

confidence: 99%

Toxicity of Transition Metal Oxide Nanoparticles: Recent Insights from in vitro Studies

2010

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“…The possible mechanism of DNA damage induced by the V 2 O 5 nanobelts/HCl/H 2 O 2 system may be illustrated as follows (Shi et al, 1996;Ivankovic et al, 2006):…”

Section: The Possible Mechanism Of Dna Damagementioning

confidence: 99%

Rapid and sensitive electrochemical sensing of DNA damage induced by V2O5 nanobelts/HCl/H2O2 system in natural dsDNA layer-by-layer films

Zhang

Yang

et al. 2010

Biosensors and Bioelectronics

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“…Pyroptosis is a form of programmed cell death of infected immune cells mediated by caspase-1 activation in an inflammasome milieu (Rosenblatt, Raff, & Cramer, 2001). Anoikis is the cell death triggered by a lack of attachment to the extracellular matrix (Malagobadan & Nagoor, 2015) and it is a regulatory mechanism important in the epithelial cell renewal (Ivankovic, Music, Gotic, & Ljubesic, 2006).…”

Section: Discussionmentioning

confidence: 99%

Vanadium compounds and cellular death mechanisms in the A549 cell line: The relevance of the compound valence

Guerrero-Palomo

Rendón-Huerta

Montaño

et al. 2018

J of Applied Toxicology

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Non‐small lung cell carcinoma has a high morbidity and mortality rates. The elective treatment for stage III and IV is cisplatinum that conveys serious toxic side effects. Vanadium compounds are metal molecules with proven antitumor activity that depends on its valence. Therefore, a better understanding of the mechanism of action of vanadium compounds is required. The aim of our study was to investigate the mechanisms of cell death induced by sodium metavanadate (NaVO3 [V(+5)]) and vanadyl sulfate (VOSO4 [(+4)]), both of which have reported apoptotic‐inducing activity. We exposed the A549 cell line to various concentrations (0‐100 μM) and to different exposure times to each compound and determined the cell viability and expression of caspases, reactive oxygen species (ROS) production, Bcl2, Bax, FasL and NO. Our results showed that neither compounds modified the basal expression of caspases or pro‐ and anti‐apoptotic proteins. The only change observed was the 12‐ and 14‐fold significant increase in ROS production induced by NaVO3 and VOSO4, respectively, at 100 μm concentrations after 48 hours. Our results suggest that classical apoptotic mechanisms are not related to the cell death induced by the vanadium compounds evaluated here, and showed that the higher ROS production was induced by the [(+4)] valence compound. It is possible that the difference will be secondary to its higher oxidative status and thus higher ROS production, which leads to higher cell damage. In conclusion, our results suggest that the efficacy of the cell death mechanisms induced by vanadium compounds differ depending on the valence of the compound.

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Cytotoxicity of nanosize V2O5 particles to selected fibroblast and tumor cells

Cited by 39 publications

References 28 publications

Toxicity of Transition Metal Oxide Nanoparticles: Recent Insights from in vitro Studies

Toxicity of Transition Metal Oxide Nanoparticles: Recent Insights from in vitro Studies

Rapid and sensitive electrochemical sensing of DNA damage induced by V2O5 nanobelts/HCl/H2O2 system in natural dsDNA layer-by-layer films

Vanadium compounds and cellular death mechanisms in the A549 cell line: The relevance of the compound valence

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