Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin

Abstract

Abstract:In this study, in vitro cytotoxicity of nickel zinc (NiZn) ferrite nanoparticles against human colon cancer HT29, breast cancer MCF7, and liver cancer HepG2 cells was examined. The morphology, homogeneity, and elemental composition of NiZn ferrite nanoparticles were investigated by scanning electron microscopy, transmission electron microscopy, and energy dispersive X-ray spectroscopy, respectively. The exposure of cancer cells to NiZn ferrite nanoparticles (15.6-1,000 µg/mL; 72 hours) has resulted in a dose-dependent inhibition of cell growth determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The quantification of caspase-3 and -9 activities and DNA fragmentation to assess the cell death pathway of the treated cells showed that both were s timulated when exposed to NiZn ferrite nanoparticles. Light microscopy examination of the cells exposed to NiZn ferrite nanoparticles demonstrated significant changes in cellular morphology. The HepG2 cells were most prone to apoptosis among the three cells lines examined, as the result of treatment with NiZn nanoparticles. In conclusion, NiZn ferrite nanoparticles are suggested to have potential cytotoxicity against cancer cells.

Green synthesis palladium nanoparticles mediated by white tea (<em>Camellia sinensis</em>) extract with antioxidant, antibacterial, and antiproliferative activities toward the human leukemia (MOLT-4) cell line

“…Caspase pathway-mediated apoptosis seems to be the common mode of cell death instituted by NPs; for example, with zerumbone-loaded nanostructured lipid carriers and magnetic iron oxide NPs on Jurkat cells, 51 amine-modified polystyrene NPs on astrocytoma cells, 52 and nickel-zinc ferrite NPs on the HepG2, HT29, and MCF-7 cell lines. 53 …”

Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release

“…The compound was formulated using a simple precipitation technique which utilized the nickel, zinc, and iron nitrates as precursors in a ratio of 0.5:0.5:2.0 following a technique described elsewhere. 32 characterization Powder X-ray diffraction patterns were recorded as previously determined 35 using a Shimadzu XRD-6000 instrument (Shimadzu Corporation, Kyoto, Japan) with CuK α radiation (λ = 1.5418 Å) and a dwell time of 4 degrees per minute. Ultraviolet spectra were recorded using a Lambda 900 ultraviolet visible spectrometer (Perkin Elmer, Waltham, MA, USA) with a scan speed of 250 nm per minute.…”

“…Transmission electron microscopic image showed the lateral dimension to be 11 nm on average as previously shown. 35 The TEM photograph of pure NiZn ferrite nanoparticles was shown in Figure 2B. It is apparent that NiZn ferrite nanoparticles are approximately …”

“…32 As a result of their superparamagnetic behavior, NiZn ferrite nanoparticles now have many potential applications, including in cell imaging 33 and cell therapy. 34 In our previous studies, 35 we have characterized NiZn ferrite nanoparticles and showed them to be toxic to HT29, MCF-7, and HepG2 cells. In the present study, the effects of NiZn ferrite nanoparticles on the generation of reactive oxygen species and their influence on glutathione and lipid peroxidation levels were determined in three cancer cell lines.…”

Physicochemical properties, cytotoxicity, and&nbsp;antimicrobial activity of sulphated zirconia&nbsp;nanoparticles

“…The Cu-K α radiation was generated by a Philips glass diffraction X-ray tube broad focus at 2.7 kW. The crystallite size D of the samples was calculated using the Debye-Scherrer's relationship: [32][33][34] …”