Cytotoxicity of nonionic amphiphilic copolymers

Budkina, Olga A.; Demina, Tatiana V.; Dorodnykh, T. Yu.; Melik‐Nubarov, N. S.; Grozdova, Irina D.

doi:10.1134/s0965545x12080020

Cited by 29 publications

(36 citation statements)

References 37 publications

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“…This result satisfactorily agrees with our recently published data concerning cytotoxicity of amphiphilic copolymers toward human breast carcinoma MCF-7/R cells (32), although the absolute values of MTC were lower by an order of magnitude. It should be noted that even though the protocol of measurements for MCF-7/R was quite different from that used in (32), the range in which the cytotoxicity of polymers varied also approached three orders of magnitude, i.e. exactly by the same manner as in the present work.…”

Section: Toxicity Of the Polymerssupporting

confidence: 92%

“…exactly by the same manner as in the present work. This finding implies that hydrophobicity of the polymer is much more important determinant of its cytotoxicity than the cell type or the experimental conditions as it has been reported previously (32).…”

Section: Toxicity Of the Polymerssupporting

confidence: 74%

“…It is known that Pluronic F127 forms small micelles (R h~1 1 nm [27]) in water solution. Commercially available samples of Pluronic F127 usually are characterized with two critical micelle concentration (CMC) values, one of which is about 1.5 lM (0.019 mg mL À1 ) and the other is much higher, about 630 lM (7.9 mg mL À1 ) (32). It is seen that the changes in Figure 6.…”

Section: Uv/vis Spectroscopic Studies Of Chlorin E6 Interaction With mentioning

confidence: 99%

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Complexes of Chlorin e6 with Pluronics and Polyvinylpyrrolidone: Structure and Photodynamic Activity in Cell Culture

Zhiyentayev

Boltaev

Соловьева

et al. 2013

Photochem & Photobiology

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Polymeric carriers are extensively used in photodynamic therapy (PDT) for increase of efficacy of photosensitizers. Here, we report the influence of nine Pluronic copolymers on phototoxicity of chlorin e6 (Ce6), in particular 5- to 7-fold rise in the phototoxicity caused by hydrophilic Pluronics F127, F108, F68 and F87 and practically no influence on Ce6 of more hydrophobic polymers. The revealed value of 0.2 mg mL(-1) of Pluronic F127 concentration sufficient for half-of-maximal increase of Ce6 photodynamic activity proved to be close to 0.16 mg mL(-1) inherent in well-documented carrier poly(N-vinylpyrrolidone) (PVP). The dissociation constants of Ce6 complexes with Pluronic F127 and PVP that were estimated from UV spectra were 0.252 and 0.036 mg mL(-1) , respectively, indicating higher stability of Ce6 complex with PVP. According to the results of (1) H-NMR studies of Ce6 complexes, the porphyrin interacts not only with hydrophobic regions but also with hydrophilic sides of both polymers.

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Section: Toxicity Of the Polymerssupporting

confidence: 92%

Section: Toxicity Of the Polymerssupporting

confidence: 74%

Section: Uv/vis Spectroscopic Studies Of Chlorin E6 Interaction With mentioning

confidence: 99%

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Complexes of Chlorin e6 with Pluronics and Polyvinylpyrrolidone: Structure and Photodynamic Activity in Cell Culture

Zhiyentayev

Boltaev

Соловьева

et al. 2013

Photochem & Photobiology

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“…The cytotoxicity of L81 has been previously reported. 40 As observed in Figure 5, at a copolymer concentration of 0.1%, the cytotoxicity increased with increasing L81 concentration (F4 < F5 < F6 < F2 < F3) in 3T3 fibroblasts (panel A) and also in Neuro-2a cells (panel B). We found that low HLB PL such as L81 are able to be inserted into lipid bilayers due to their hydrophobicity, promoting changes in their structure and function, 41 which could explain at least in part the observed cytotoxicity.…”

Section: ■ Experimental Sectionmentioning

confidence: 53%

Pluronics F-127/L-81 Binary Hydrogels as Drug-Delivery Systems: Influence of Physicochemical Aspects on Release Kinetics and Cytotoxicity

et al. 2014

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We investigated the structure of the binary mixture of Pluronic F-127 (PL F-127) and Pluronic L-81 (PL L-81), as hydrogels for sumatriptan delivery and investigated the mixture possible use via subcutaneous route for future applications as a long-acting antimigraine formulation. We studied the drug-micelle interaction by dynamic light scattering and differential scanning calorimetry, sol-gel process by rheology, and small-angle X-ray scattering (SAXS). We also employed pharmaceutical formulation aspects by dissolution rate, release profile, and cytotoxicity studies for apoptosis and/or necrosis in fibroblasts (3T3) and neural cells (Neuro 2a). Micellar hydrodynamic diameter studies revealed the formation of binary PL-micelles by association of PL F-127/PL L-81. The mixed micelle and binary hydrogels formation was also verified by only one phase transition temperature for all formulations, even in the presence of sumatriptan. The characterization of the hydrogel supramolecular organization by SAXS, rheology studies, and in vitro dissolution/release results showed a probable relationship between the transition of the lamellar to the hexagonal phase and the lower release constant values observed, indicating that PL L-81 participates in micelle-hydrogel formation and aggregation processes. Furthermore, the reduced cytotoxicity (annexin V-fluorescein isothiocyanate positive staining), with minor PL L-81 concentration, points to its potential use for the development of binary PL-systems containing sumatriptan capable of modulating the gelation process. This use may employ the minimum PL concentration and be interesting for pharmaceutical applications, particularly for migraine treatment.

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“…3 H‐labeled copolymers were incubated with MDR human breast adenocarcinoma MCF‐7/ADR cells. This cell line was chosen because it has been routinely used in our group for the analysis of cytotoxicity and chemosensitizing properties of the copolymers studied in this report …”

Section: Resultsmentioning

confidence: 99%

Increase in the length of poly(ethylene oxide) blocks in amphiphilic copolymers facilitates their cellular uptake

Grozdova

Badun

Chernysheva

et al. 2017

J of Applied Polymer Sci

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Amphiphilic nonionic block copolymers induce different biological effects on living cells. In particular, the hydrophobic members of the Pluronics family suppress drug efflux systems in multidrug‐resistant cells, whereas hydrophilic Pluronics support cell viability. However, the relationship between the copolymer structure and its binding to cells is still unclear. Using a tritium‐labeling approach, we analyzed interactions of nine Pluronics and three diblock copolymers with human and murine cells in vitro and revealed that the binding efficiency of the copolymers increased in line with the length of their poly(ethylene oxide) (PEO) blocks. In contrast, partitioning of the same polymers into artificial lipid bilayers determined by Isothermal Titration Calorimetry (ITC) decreased with increasing length of the PEO block. The opposite influence of hydrophilic blocks on the copolymer affinity to living cells and artificial lipid bilayers implied the binding of long PEO blocks to the hydrophilic moieties of cellular membranes. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45492.

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Cytotoxicity of nonionic amphiphilic copolymers

Cited by 29 publications

References 37 publications

Complexes of Chlorin e6 with Pluronics and Polyvinylpyrrolidone: Structure and Photodynamic Activity in Cell Culture

Complexes of Chlorin e6 with Pluronics and Polyvinylpyrrolidone: Structure and Photodynamic Activity in Cell Culture

Pluronics F-127/L-81 Binary Hydrogels as Drug-Delivery Systems: Influence of Physicochemical Aspects on Release Kinetics and Cytotoxicity

Increase in the length of poly(ethylene oxide) blocks in amphiphilic copolymers facilitates their cellular uptake

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