Cytotoxicity of Protein-Carbon Nanotubes on J774 Macrophages Is a Functionalization Grade-Dependent Effect

Montes-Fonseca, Silvia Lorena; Sánchez‐Ramírez, Blanca; Luna-Velasco, Antonia; Arzate-Quintana, Carlos; Silva-Cázares, Macrina Beatriz; Horta, Carmen González; Orrantia‐Borunda, Erasmo

doi:10.1155/2015/796456

Cited by 7 publications

(4 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cytotoxic effect of CNTs functionalized with a 46-kDa surface protein was evaluated in J774A macrophages. The results showed that functionalized P46-CNTs displayed grade-dependent cytotoxicity, that is, P46-CNTs of high grade were more toxic than those of low grade [ 77 ]. Additionally, the nature of the functional protein influenced the cytotoxicity of nanoparticles.…”

Section: Main Textmentioning

confidence: 99%

Cellular Toxicity and Immunological Effects of Carbon-based Nanomaterials

et al. 2019

View full text Add to dashboard Cite

Background Carbon nanomaterials are a growing family of materials featuring unique physicochemical properties, and their widespread application is accompanied by increasing human exposure. Main body Considerable efforts have been made to characterize the potential toxicity of carbon nanomaterials in vitro and in vivo. Many studies have reported various toxicology profiles of carbon nanomaterials. The different results of the cytotoxicity of the carbon-based materials might be related to the differences in the physicochemical properties or structures of carbon nanomaterials, types of target cells and methods of particle dispersion, etc. The reported cytotoxicity effects mainly included reactive oxygen species generation, DNA damage, lysosomal damage, mitochondrial dysfunction and eventual cell death via apoptosis or necrosis. Despite the cellular toxicity, the immunological effects of the carbon-based nanomaterials, such as the pulmonary macrophage activation and inflammation induced by carbon nanomaterials, have been thoroughly studied. The roles of carbon nanomaterials in activating different immune cells or inducing immunosuppression have also been addressed. Conclusion: Here, we provide a review of the latest research findings on the toxicological profiles of carbon-based nanomaterials, highlighting both the cellular toxicities and immunological effects of carbon nanomaterials. This review provides information on the overall status, trends, and research needs for toxicological studies of carbon nanomaterials.

show abstract

Section: Main Textmentioning

confidence: 99%

Cellular Toxicity and Immunological Effects of Carbon-based Nanomaterials

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Our results agree with this because f-CNTs did not decrease cell viability at the tested doses at 1 or 24 h of interaction in SKOV-3 or J774A.1 cell lines. These results are critical for their potential biomedical application, as the protein-CNTs bio-conjugation would decrease their cytotoxicity in in vivo models [47,48].…”

Section: Peptide Design and F-cnts Physicochemical Characterizationmentioning

confidence: 99%

Non-cytotoxic carbon nanotubes bioconjugated with fucosyltransferase 4-derived peptides modulate macrophage polarization in vitro

Guzmán-Mendoza

Sánchez‐Ramírez

Cigarroa-Mayorga

et al. 2023

Preprint

View full text Add to dashboard Cite

In recent years, nanotechnology has had an important development in nanoparticle-based therapies. Carbon nanotubes (CNTs) are among the most valuable nanoparticles, given their physicochemical properties and functionalization possibilities; therefore, they are proposed as peptide carriers in immunotherapies. Immunotherapy has been explored as a promising therapy in ovarian cancer (OvCa), and it has been reported that macrophage polarization into M1 and M2 phenotypes plays a pivotal role in OvCa initiation, progression, and metastasis, providing therapeutic targets for macrophage-targeted treatment. In this work, we explored the initial stages for the design of CNTs-based immunotherapy for ovarian cancer (OvCa) using fucosyltransferase-4-derived T cell epitopes conjugated with CNTs (f-CNTs). Their cytotoxicity and biological interactions were analyzed in macrophages (J774A.1) and human ovarian cancer cells (SKOV-3). Here we showed that f-CNTs do not show cytotoxicity signs in concentrations < 6 µg/mL; additionally, they induced morphological changes and activation in macrophages, time-dependent uptake in lysosomes, production of M1-like cytokines, upregulation of CD80, CD86, and MHC II, and downregulation of ARG-1. In conclusion, f-CNTs exhibited biocompatibility in both cell lines and displayed M1-like polarization in macrophages, allowing us to propose them as a peptide carrier system for macrophage activation and polarization for being explored in ovarian cancer immunotherapies.

show abstract

“…The purification process was carried out by sonication for 48 h in a mixture of concentrated H 2 SO 4 (90%)/HNO 3 (70%), (J.T. Baker, Loughborough, Leicestershire, UK) 3:1 v/v to afford P-CNTs [17].…”

Section: Nanoparticlesmentioning

confidence: 99%

“…Our research group has demonstrated that purification processes, functionalization, and the nature of protein functionalization are important factors to consider during in vitro f-CNT cytotoxicity studies [15][16][17]. In order to be used in nanomedicine applications such as nanovaccines or drug delivery among others, in vivo studies are required to produce safer nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Safe Administration of Carbon Nanotubes by Intravenous Pathway in BALB/c Mice

et al. 2020

Self Cite

View full text Add to dashboard Cite

Carbon nanotubes (CNTs) are nanomaterials with multiple possible uses as drug carriers or in nanovaccine development. However, the toxicity of CNTs administered intravenously in in vivo models has not been fully described to date. This work aimed to evaluate the toxic effect of pristine multi-walled CNTs (UP-CNTs), purified (P-CNTs), or CNTs functionalized with fluorescein isothiocyanate (FITC-CNTs) administered by intravenous injection in BALB/c mice. Biochemical and histopathological parameters were analyzed at 1, 14, 29, and 60 days post-exposure. Pristine CNTs were the most toxic nanoparticles in comparison with P-CNTs or FITC-CNTs, increasing serum AST (≈ 180%), ALT (≈ 300%), and LDH (≈ 200%) levels at one day post-exposure. The urea/creatinine ratio suggested pre-renal injury at the 14th day accompanied of extensive lesions in kidneys, lungs, and liver. Biochemical and histological findings in mice exposed to P-CNTs had not significant differences compared to the controls. A lower toxic effect was detected in animals exposed to FITC-CNTs which was attributable to FITC toxicity. These results demonstrate that the purification process of CNTs reduces in vivo toxicity, and that toxicity in functionalized CNTs is dependent on the functionalized compound. Therefore, P-CNTs are postulated as potential candidates for safe biomedical applications using an intravenous pathway.

show abstract

Cytotoxicity of Protein-Carbon Nanotubes on J774 Macrophages Is a Functionalization Grade-Dependent Effect

Cited by 7 publications

References 28 publications

Cellular Toxicity and Immunological Effects of Carbon-based Nanomaterials

Cellular Toxicity and Immunological Effects of Carbon-based Nanomaterials

Non-cytotoxic carbon nanotubes bioconjugated with fucosyltransferase 4-derived peptides modulate macrophage polarization in vitro

Safe Administration of Carbon Nanotubes by Intravenous Pathway in BALB/c Mice

Contact Info

Product

Resources

About