Cytotoxicity of seputhecarpan D, thonningiol and 12 other phytochemicals from African flora towards human carcinoma cells

Kuete, Victor; Ngnintedo, Dominique; Fotso, Ghislain Wabo; Karaosmanoğlu, Oğuzhan; Ngadjui, Bonaventure T.; Keumedjio, Félix; Yeboah, Samuel O.; Andrae‐Marobela, Kerstin; Sivas, Hülya

doi:10.1186/s12906-018-2109-9

Cited by 18 publications

(5 citation statements)

References 38 publications

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“…Our study corresponds with other studies dealing with these chemicals. For example, in the study by Kuete et al (2018), ß-amyrin produced a selective cytotoxic effect against Caco-2 compared to that on normal cell line HEK293. In another study, extract of Alstonia macrophylla Wall.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Selective Antibacterial and Antiproliferative Effects of Ethanolic Extracts from Cambodian and Philippine Plants Used in Folk Medicine for Diarrhea Treatment

et al. 2021

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Bacterial diarrhea remains a global health problem, especially in developing tropical countries. Moreover, dysbiosis caused by diarrheagenic bacteria and inappropriate antimicrobial treatment has been associated with intestinal carcinogenesis. Despite the rich tradition of the use of herbs for the treatment of gastrointestinal disorders in Cambodian and Philippine folk medicine, many of them have not yet been systematically studied for their in vitro selective inhibitory effects on intestinal bacteria and cells. In the present study, in vitro inhibitory activities of 35 ethanolic extracts derived from 32 Cambodian and Philippine medicinal plants were determined by broth microdilution method against 12 pathogenic bacteria. Furthermore, cytotoxicity against intestinal cancer cells (Caco-2 and HT-29) using thiazolyl blue tetrazolium bromide cytotoxicity assay and safety to six beneficial intestinal bacteria (bifidobacteria and lactobacilli) and intestinal normal cells (FHs 74 Int) were determined for the antimicrobially active extracts. Selectivity indices (SIs) were calculated among the averages of minimum inhibitory concentrations (MICs), half-maximal inhibitory concentrations (IC50), and 80% inhibitory concentrations of proliferation (IC80) for each type of the tested agents. The extracts of Artocarpus blancoi (Elmer) Merr. (Moraceae), Ancistrocladus tectorius (Lour.) Merr. (Ancistrocladaceae), and Pentacme siamensis (Miq.) Kurz (Dipterocarpaceae) produced significant growth-inhibitory effects (MICs = 32–512 μg/ml) against intestinal pathogenic bacteria at the concentrations nontoxic to normal intestinal cells (IC80 values >512 μg/ml; SIs = 0.11–0.2). Moreover, the extract of P. siamensis (Miq.) Kurz was relatively safe to beneficial bacteria (MICs ≥512 μg/ml; SI = 0.1), and together with A. blancoi (Elmer) Merr., they selectively inhibited intestinal cancer cells (IC50 values ≥51.98 ± 19.79 μg/ml; SIs = 0.3 and 0.6). Finally, a strong selective antiproliferative effect on cancer cells (IC50 values 37.89 ± 2.68 to 130.89 ± 13.99 μg/ml; SIs = 0.5) was exerted by Ehretia microphylla Lam. (Boraginaceae), Lagerstroemia cochinchinensis Pierre ex Gagnep. (Lythraceae), and Melastoma saigonense (Kuntze) Merr. (Melastomataceae) (leaves with flower buds). The results suggest that the above-mentioned species are promising materials for the development of new selective antibacterial and antiproliferative agents for the treatment of infectious diarrhea and associated intestinal cancer diseases. However, further research is needed regarding the isolation and identification of their active constituents.

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Section: Discussionmentioning

confidence: 99%

In Vitro Selective Antibacterial and Antiproliferative Effects of Ethanolic Extracts from Cambodian and Philippine Plants Used in Folk Medicine for Diarrhea Treatment

et al. 2021

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“…Outro estudo descreveu que a curcumina foi capaz de atuar como potente inibidor de NF-kß (fator nuclear kappa ß), podendo ser caracterizado como potente agente anticâncer, uma vez que esse complexo proteico (NF-kß) está relacionado à reposta de estímulos de estresse e sua desregulação tem ligação com o desenvolvimento de tumores 12,76 . Além disso, a curcumina foi associada à parada do ciclo celular na fase G2/S, induzindo apoptose.…”

Section: Discussionunclassified

“…O ácido ursólico, um triterpenoide pentacíclico identificado nas ceras epicuticulares das maçãs e amplamente encontrado nas cascas das frutas e plantas medicinais, foi capaz de induzir apoptose nas células cancerosas do câncer de pele por meio do aumento da concentração de capazes, em especial a caspase número 3 efetora, responsável por clivar substratos, dando sequência à cascata que leva à morte celular 76 . Os resultados encontrados mostraram uma eficácia dose-dependente, o que sugere que a aplicação coadjuvante ao tratamento de câncer deverá ser feita com o composto isolado, com o intuito de potencializar os efeitos da terapia convencional 91 .…”

Section: Discussionunclassified

Plantas Medicinais e seus Compostos com Potencial Terapêutico no Tratamento do Câncer: Revisão Integrativa

Feitoza

Terra

Grasselli

2021

Rev. Bras. Cancerol.

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Introdução: As transições epidemiológica e nutricional têm contribuído para o crescimento exponencial do câncer. Assim, com o intuito de potencializar a atividade das drogas antineoplásicas, aumentou o número de estudos utilizando plantas medicinais para tratamento do câncer. Objetivo: Identificar as plantas medicinais e os compostos com possível ação no processo de tratamento do câncer. Método: Foi realizada uma revisão integrativa. Para a busca bibliográfica, foram utilizados os descritores “neoplasias” e “plantas medicinais” e o qualificador “tratamento” para a palavra câncer, especificando-a. O levantamento dos artigos foi feito nas seguintes bases de dados: Scientific Electronic Library OnLine (SciELO), Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS) e Medical Literature Analysis and Retrieval Sistem Online (MEDLINE) via PubMed. Resultados: A busca e a aplicação dos critérios de inclusão e exclusão resultaram na seleção de 93 artigos. As principais características dos estudos incluídos eram ensaios in vitro, com a linhagem de células de câncer de mama e publicados em inglês. Foi possível identificar seis compostos que se destacaram em razão da sua atividade pró-apoptótica em vários tipos de câncer, a exemplo da curcumina, da epicatequina, do lupeol, do ácido cafeico, do ácido ursólico e da berberina. Conclusão: O presente trabalho oferece subsídios para que pesquisas futuras possam associar a utilização de compostos presentes em plantas medicinais ao tratamento convencional, com o intuito de melhorar o prognóstico de pacientes oncológicos.

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“…Glyasperin F may regulate cell growth by affecting the activation of MMP1 Glyasperin F is an isoflavone compound, studies have found that Glyasperin F can inhibit the proliferation of lung cancer cells (Ngnintedo et al, 2016;Kuete et al, 2018).…”

Section: Glycyrol May Affect Cell Proliferation and Survival By Regul...mentioning

confidence: 99%

Exploring the mechanism of action of licorice in the treatment of COVID-19 through bioinformatics analysis and molecular dynamics simulation

Cao

Gong²,

Wu³

et al. 2022

Front. Pharmacol.

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Purpose: The rapid worldwide spread of Corona Virus Disease 2019 (COVID-19) has become not only a global challenge, but also a lack of effective clinical treatments. Studies have shown that licorice can significantly improve clinical symptoms such as fever, dry cough and shortness of breath in COVID-19 patients with no significant adverse effects. However, there is still a lack of in-depth analysis of the specific active ingredients of licorice in the treatment of COVID-19 and its mechanism of action. Therefore, we used molecular docking and molecular dynamics to explore the mechanism of action of licorice in the treatment of COVID-19.Methods: We used bioinformatics to screen active pharmaceutical ingredients and potential targets, the disease-core gene target-drug network was established and molecular docking was used for verification. Molecular dynamics simulations were carried out to verify that active ingredients were stably combined with protein targets. The supercomputer platform was used to measure and analyze stability of protein targets at the residue level, solvent accessible surface area, number of hydrogen bonds, radius of gyration and binding free energy.Results: Licorice had 255 gene targets, COVID-19 had 4,628 gene targets, the intersection gene targets were 101. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene ontology (GO) analysis showed that licorice played an important role mainly through the signaling pathways of inflammatory factors and oxidative stress. Molecular docking showed that Glycyrol, Phaseol and Glyasperin F in licorice may playe a role in treating COVID-19 by acting on STAT3, IL2RA, MMP1, and CXCL8. Molecular dynamics were used to demonstrate and analyze the binding stability of active ingredients to protein targets.Conclusion: This study found that Phaseol in licorice may reduce inflammatory cell activation and inflammatory response by inhibiting the activation of CXCL8 and IL2RA; Glycyrol may regulate cell proliferation and survival by acting on STAT3. Glyasperin F may regulate cell growth by inhibiting the activation of MMP1, thus reducing tissue damage and cell death caused by excessive inflammatory response and promoting the growth of new tissues. Therefore, licorice is proposed as an effective candidate for the treatment of COVID-19 through STAT3, IL2RA, MMP1, and CXCL8.

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Cytotoxicity of seputhecarpan D, thonningiol and 12 other phytochemicals from African flora towards human carcinoma cells

Abstract: Background: Despite the remarkable progress in cancer therapy

Cited by 18 publications

References 38 publications

In Vitro Selective Antibacterial and Antiproliferative Effects of Ethanolic Extracts from Cambodian and Philippine Plants Used in Folk Medicine for Diarrhea Treatment

In Vitro Selective Antibacterial and Antiproliferative Effects of Ethanolic Extracts from Cambodian and Philippine Plants Used in Folk Medicine for Diarrhea Treatment

Plantas Medicinais e seus Compostos com Potencial Terapêutico no Tratamento do Câncer: Revisão Integrativa

Exploring the mechanism of action of licorice in the treatment of COVID-19 through bioinformatics analysis and molecular dynamics simulation

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