Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties

Sadı, Gökhan; Emsen, Bugrahan; Kaya, Abdullah; Kocabaş, Aytaç; Çınar, Seval; Kartal, Deniz İrtem

doi:10.4103/0973-1296.157665

Cited by 36 publications

(17 citation statements)

References 60 publications

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“…The antiradical scavenging activity of the D. tripetala aqueous seed extracts mentioned in this study is owing to the presence of phenols that usually possesess high metal-chelating activity. This result correlates with the reports of Jazek et al [30], Sadi et al, [31] and Ojo et al [32,33]. Tables 1 and 2].…”

Section: Discussionsupporting

confidence: 92%

Dennettia tripetala seeds inhibit ferrous sulphate-induced oxidative stress in rat tissues in vitro

Oyetayo¹,

Ojo²

2017

Oxid Antioxid Med Sci

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Section: Discussionsupporting

confidence: 92%

Dennettia tripetala seeds inhibit ferrous sulphate-induced oxidative stress in rat tissues in vitro

Oyetayo¹,

Ojo²

2017

Oxid Antioxid Med Sci

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“…Hericium erinaceus has been demonstrated to play a role in chemotherapy resistance against HCC by facilitating intracellular doxorubicin accumulation through the inhibition of the NF-κB activity [11]. Extracts of edible mushroom have indicated considerable cytotoxic, antibacterial, and antioxidant properties in HCC HepG2 and may be utilized as a potent anti-infection drug against HCC [31]. Another study showed that the Akt/GSK-3β pathway facilitates the lowering of the CD44 and TGF-b1 migration in HCC cells [32].…”

Section: Discussionmentioning

confidence: 99%

Erinacine Facilitates the Opening of the Mitochondrial Permeability Transition Pore Through the Inhibition of the PI3K/ Akt/GSK-3β Signaling Pathway in Human Hepatocellular Carcinoma

Zhou

et al. 2018

Cell Physiol Biochem

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Background/Aims: Erinacine, which is extracted from the medicinal mushroom Hericium erinaceus, is known to play anticancer roles in human cancers. The following study aims to investigate the role of erinacine in the opening of the mitochondrial permeability transition pore (MPTP) in hepatocellular carcinoma (HCC) through the PI3K/Akt/GSK-3β signaling pathway and highlights the applicability of erinacine in HCC treatments. Methods: HCC and paracancerous tissues were obtained from 85 HCC patients who’ve undergone surgical resection. Immunohistochemistry was adopted to detect positive expression of PI3K, Akt, and GSK-3β. Treatment of HepG-2 with LY294002 (an inhibitor of the PI3K/Akt/GSK-3β signaling pathway) and different concentration of erinacine was performed to determine the involvement of LY294002 in erinacine action. The expressions of PI3K, Akt, GSK-3β, CyclinD1, Vimentin, β-catenin, Bcl-2, E-cadherin, Bax, and caspase-9 were determined by RT-qPCR and Western blot analysis. Cell viability, colony formation rate, migration, invasion, cycle, and apoptosis were detected by MTT, colony formation, wound healing assay, Transwell assay, and flow cytometry, respectively. The size and weight of xenograft tumors were observed in nude mice. Mitochondrial membrane potential in HepG-2 was determined using laser scanning confocal microscopy following JC-1 staining. Mitochondrial Ca²⁺ indicator Rhod-2, AM was used to detect the changes of mitochondrial Ca²⁺, while western blot analysis was employed to detect the presence levels of cytochrome C (cyt-C). Results: The results revealed that PI3K, Akt, and GSK-3β were up-regulated in HCC tissues. Erinacine or LY294002 led to a decrease in mitochondrial membrane potential, increase in intracellular mitochondrial Ca²⁺, and the release of cyt-C in mitochondria. In addition, Erinacine was found to decrease the mitochondrial membrane potential, expression of PI3K, Akt, GSK-3β, CyclinD1, Vimentin, β-catenin, and Bcl-2, cell proliferation, colony formation ability, migration, invasion, and xenograft tumor size, while E-cadherin, Bax, and caspase-9 expression, and cell apoptosis were elevated in a dose-dependent manner. Erinacine also stimulated the effects of LY294002 on the HCC. Following the addition of 500 μM Erinacine and MPTP opening inhibitor CsA, we found that the mitochondrial membrane potential level increased, while mitochondrial Ca2+ and Cyt-C decreased from the mitochondria. Conclusion: The results from the study demonstrated that erinacine induced MPTP opening, facilitates the release of cyt-C, and inhibited cell proliferation, migration, and invasion, while it promotes apoptosis by inactivating the PI3K/Akt/GSK-3β signaling pathway, preventing the progression of HCC.

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“…The IC 50 values of methanol and acetone extracts of T. focale, however, were 0.21 ± 0.01, and 0.40 ±0.01 mg/mL. Ferreira et al (2007) privously tested DPPH scavenging activity of methanol extract of another wild edible mushroom T. portentosum and found IC 50 as 40.2 mg/mL and Sadi et al (2015) found IC 50 as 3.25 mg/mL for T. fracticum which was not even close to T.imbricatum extracts. Tel et al (2011) worked on wild mushroom species namely T. fracticum, T. terreum and T. imbricatum with different extracts our result were comparable according to ABTS test.…”

Section: Antioxidant Activity Resultsmentioning

confidence: 99%

“…The various phytochemicals were isolated form the T. matsutake exhibiting anti-inflammatory, antioxidant and anticancer activities (Ding and Hou, 2012;Taofiq et al, 2016;You et al, 2013). Besides this species other Tricholoma species have been also attracted (Chen et al, 2017;Hassan et al, 2015;Sadi et al, 2015). Having the similar aim, we have performed complimentary antioxidant activity tests in addition to fatty acid analysis of T. imbricatum and T. focale to increase the knowledge.…”

Section: Introductionmentioning

confidence: 99%

Fatty Acid Composition and Antioxidant Activity of Tricholoma Imbricatum and T. Focale

Kaplaner

Singeç

Öztürk

2017

Turkish JAF Sci.Tech.

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Edible mushrooms are one of the important food source because of their tastes, and having high concentration of fatty acids and the other nutrients. In this study, fatty acid composition and antioxidant properties of Tricholoma imbricatum and T. focale were determined. The fatty acids were analysed by GC, and GC-MS while the antioxidant activity was tested using five complimentary methods; namely, β-carotene-linoleic acid, DPPH scavenging, ABTS scavenging, CUPRAC and metal chelating assays. Eight fatty acids were determined in hexane extract, and oleic acid was the major fatty acid with 46.4% and 35.0%, respectively. Palmitic acid (12.8%, and 5.12%) and lineloic acid (28.2% and 31.0%) were also detected in high amounts. In DPPH . scavenging and ABTS ·+ scavenging assays, the methanol extract of T. imbricatum (IC 50 : 0.12±0.01 mg/mL for both assay) showed better antioxidant activity than those of T. focale (IC 50 >0.8±0.01, and IC 50 : 0.21±0.01 mg/mL). All extracts of T. focale exhibited good activity in β-carotenelinoleic acid assay while only the hexane extract of T. imbricatum showed activity. Both mushroom indicated moderate in cupric reducing power. Since the mushrooms are nontoxic and edible, both can be used in food industry as preservatives.

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Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties

Cited by 36 publications

References 60 publications

Dennettia tripetala seeds inhibit ferrous sulphate-induced oxidative stress in rat tissues in vitro

Dennettia tripetala seeds inhibit ferrous sulphate-induced oxidative stress in rat tissues in vitro

Erinacine Facilitates the Opening of the Mitochondrial Permeability Transition Pore Through the Inhibition of the PI3K/ Akt/GSK-3β Signaling Pathway in Human Hepatocellular Carcinoma

Fatty Acid Composition and Antioxidant Activity of Tricholoma Imbricatum and T. Focale

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