Cytotoxicity of Some Plants of the Asteraceae Family: Antiproliferative Activity of Psiadia punctulata Root Sesquiterpenes

Abstract

According to the World Health Organization Cancer is the second cause of death globally. The methanol extracts of fourteen Middle-Eastern plants of the family Asteraceae were screened for antiproliferative activity on five cancer cell lines (A2780, MCF7, HeLa, RKO and Jurkat) by using MTT assay. Psiadia punctulata (DC.) Vatke was selected for isolation and elucidation of the bioactive constituents by 1D-and 2D-NMR, and MS analyses. Flow cytometry was used to evaluate cell cycle analysis, apoptotic hallmarks and reactive oxygen species. P. punctulata yielded a new sesquiterpene characterized as 7-hydroxy eudesman-3,5-dien-2-one (punctulin) (3) and three known sesquiterpenes: 1, 2 and 4. The antiproliferative activity of all sesquiterpenes was evaluated in Jurkat (T-cell leukemia) and HeLa cancer cell lines. 1β-hydroxy-8-oxo-cyperone (1) has induced a significant growth inhibition in Jurkat and HeLa cells (IC50 =12 and 18µM respectively). Flow cytometry of compound 1 has elucidated the mechanism of action by showing its ability to induce cell cycle arrest in Jurkat cells mainly in G0/G1 and, less markedly, in G2/M. Compound 1 also expressed strong antioxidant activity by reducing the basal level of peroxides DHFDA-load in Jurkat cells. Antioxidant activity of compound 1 may have contributed to the observed cell cycle arrest.

Antimicrobial Potential of Biosynthesized Silver Nanoparticles by Aaronsohnia factorovskyi Extract

“…Aaronsohnia factorovskyi (A. factorovskyi ) is a member of Asteraceae family, which includes several medicinal plants, such as Matricaria chamomilla and Helianthus annuus (sunflower) that are characterized by their rich content of sesquiterpenes, flavonoids, and coumarins, which are active constituents of interesting therapeutic importance [ 4 ]. Recently, the flora of the Middle East has attracted the attention of several researchers and scientists to investigate their biological activities, particularly the antimicrobial and antitumor effects [ 5 ].…”

Proapoptotic Activity of Achillea membranacea Essential Oil and Its Major Constituent 1,8-Cineole against A2780 Ovarian Cancer Cells

“…In a previous published study, we reported the marginal cytotoxic effect of the methanol extract of the aerial parts of A. membranacea on Jukart cells, with an IC 50 value of 60 ± 5.1 µg/mL [15]. That extract, however, did not exert any cytotoxic effect on MCF7 or A2780 cancer cell lines (IC 50 > 200 µg/mL) [23]. The growth of A2780 cells in this study was, however, significantly inhibited (IC 50 = 12.99 µg/mL) by A. membranacea EO, since the natural product components are generally considered cytotoxic when their IC 50 is ≤ 20 µg/mL [38].…”

“…Casticin, which was isolated from A. millefolium L. through HPLC, showed cytotoxic, early apoptosis, G 2 /M cell cycle arrest and consequently disruption of the mitotic spindle in two MCF7 sublines [22]. In a previous study, the methanol extract of A. membranacea exerted a moderate cytotoxic effect upon Jurkat cells (T-cell leukemia) using the MTT assay [23].…”

Synthesis and Antiproliferative Activity of 4β‐O‐Substituted, 4β‐N‐Substituted Deoxypodophyllotoxin Derivatives, and 4β‐OH‐ 4’‐O‐Substituted Podophyllotoxin

“…As described in the experimental section (supplementary material), the MTT test was performed against four cancer cell lines specifically MCF-7, Caco-2, MDA231, T47D, [17,18] and one normal cell line, namely skin Fibroblasts (PCS201012). [19,20] which was selected as a reference of normal cells to evaluate the safety of our products as reported in table 1.…”