Cytotoxicity of substances leached or dissolved from pulp capping materials

Cavalcanti, Bruno das Neves; Rode, Sigmar de Mello; Marques, Márcia Martins

doi:10.1111/j.1365-2591.2005.00967.x

Cited by 69 publications

(69 citation statements)

References 14 publications

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“…21 Moreover, it has been previously shown that Single Bond is cytotoxic to human dental pulp fibroblasts. 22 In a recent study, this material also induced angiogenic growth factors. 23 Thus, these results indicate a non-beneficial effect of adhesive systems used as pulp capping materials, at least in proximity to the dental pulp.…”

Section: Discussionmentioning

confidence: 99%

Pulp capping materials exert an effect on the secretion of IL-1β and IL-8 by migrating human neutrophils

et al. 2011

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Pulp repair is a complex process whose mechanisms are not yet fully understood. The first immune cells to reach the damaged pulp are neutrophils that play an important role in releasing cytokines and in phagocytosis. The objective of this study was to analyze the effect of different pulp-capping materials on the secretion of interleukin-1 beta (IL-1β) and interleukin-8 (IL-8) by migrating human neutrophils. Neutrophils were obtained from the blood of three healthy donors. The experimental groups were calcium hydroxide [Ca(OH) 2 ], an adhesive system (Single Bond), and mineral trioxide aggregate (MTA). Untreated cells were used as control. Transwell chambers were used in performing the assays to mimic an in vivo situation of neutrophil chemotaxis. The pulpcapping materials were placed in the lower chamber and the human neutrophils, in the upper chamber. The cells were counted and the culture medium was assayed using ELISA kits for detecting and quantifying IL-1β and IL8. The data were compared by ANOVA followed by Tukey's test (p < 0.05). The secretion of IL-8 was significantly higher in all groups in comparison to the control group (p < 0.05). The adhesive system group showed higher IL-8 than the MTA group (p < 0.05). The secretion of IL-1β was significantly greater only in the MTA group (p < 0.001). It was concluded that only MTA is able to improve the secretion of IL-1β, and all materials tested increased IL-8 secretion. These results combined with all the other biological advantages of MTA indicate that it could be considered the material of choice for dental pulp capping.

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Section: Discussionmentioning

confidence: 99%

Pulp capping materials exert an effect on the secretion of IL-1β and IL-8 by migrating human neutrophils

et al. 2011

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“…The culture medium conditioned by the bleaching agent was obtained as described by Cavalcanti et al 26 For obtaining the conditioned medium, 0.2 g of 35% H 2 O 2 (Ultradent, South Jordan, UT, USA) per 1 mL of culture medium was used. The conditioning time was 30 minutes, and immediately after conditioning the conditioned medium diluted to 10 …”

Section: Conditioned Mediummentioning

confidence: 99%

In vitro effect of low intensity laser on the cytotoxicity produced by substances released by bleaching gel

et al. 2010

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This in vitro study aimed to analyze the effect of different parameters of phototherapy with low intensity laser on the viability of human dental pulp fibroblasts under the effect of substances released by bleaching gel. Cells were seeded into 96 wells plates (1 x 10 3 cells/well) and placed in contact with culture medium conditioned by a 35% hydrogen peroxide bleaching gel for 40 minutes, simulating the clinical condition of the in-office bleaching treatment. Cells cultured in ideal growth conditions served as positive control group (PC), and the cells grown in conditioned medium and non-irradiated served as negative control group (NC). Cells grown in conditioned medium were submitted to a single irradiation with a diode laser (40 mW, 0.04 cm²) emitting at visible red (660 nm; RL) or near infrared (780 nm; NIR) using punctual technique, in contact mode and energy densities of 4, 6 or 10 J/cm². The cell viability was analyzed through the MTT reduction assay immediately and 24 hours after the irradiation. The data was compared by ANOVA followed by the Tukey's test (p ≤ 0.05). The cell viability increased significantly in 24 hours within each group. The PC presented cell viability significantly higher than NC in both experimental times. Only the NIR/10 J/ cm² group presented cell viability similar to that of PC in 24 hours. The phototherapy with low intensity laser in defined parameters is able to compensate the cytotoxic effects of substances released by 35% hydrogen peroxide bleaching gel.

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“…The release of unreacted monomers from resin composites may stimulate the growth of bacteria [18] and promote allergic or cytotoxic reactions to intraoral tissues [18]. Diffusion of water into resin matrices may result in the deterioration of the physical/ mechanical properties, decreasing the life expectancy of the interfaces by hydrolysis and microcrack formation [19,20]. Water ingress may, however, have related beneficial effects [19,20].…”

Section: Discussionmentioning

confidence: 99%

“…Diffusion of water into resin matrices may result in the deterioration of the physical/ mechanical properties, decreasing the life expectancy of the interfaces by hydrolysis and microcrack formation [19,20]. Water ingress may, however, have related beneficial effects [19,20].…”

Section: Discussionmentioning

confidence: 99%

Testing endodontic sealers on human umbilical vein endothelial cells

et al. 2013

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AbstractThe toxic potential of the endodontic sealers ingredients, especially the unreacted monomer, that can irritate the periapical tissue and interfere with the healing process, thus having a negative impact on the biocompatibility of the material. The aim of this study was to evaluate the influence of three experimental endodontic sealers on cells viability in vitro. Human umbilical vein endothelial cells (HUVEC) were used. The experiments were done with solid samples and extracts of sealers in artificial saliva and water. The experiments evaluated the cytotoxicity of the residual monomers that resulted from the tested composites. The decrease in cell viability was quantified by colorimetric measurement of formazan. The components of the sealers dissolved in artificial saliva and water were determined by high performance liquid chromatography (HPLC). The HUVEC are a novelty for testing the endodontic sealers biocompatibility, with certain advantages compared to other cell types used in the literature, e.g. HELA cells, fibroblasts. The data showed that cytotoxicity was directly linked with the unreacted monomer — 2-hydroxyethyl methacrylate (HEMA) present in these composites. Two of the three formulations had little or no cytotoxic effect, which makes them suitable for further testing in order to be used in endodontic treatment.

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Cytotoxicity of substances leached or dissolved from pulp capping materials

Abstract: Substances dissolved from the adhesive system tested were cytotoxic for human dental pulp fibroblasts in culture, whilst substances leached from calcium hydroxide were biocompatible.

Cited by 69 publications

References 14 publications

Pulp capping materials exert an effect on the secretion of IL-1β and IL-8 by migrating human neutrophils

Pulp capping materials exert an effect on the secretion of IL-1β and IL-8 by migrating human neutrophils

In vitro effect of low intensity laser on the cytotoxicity produced by substances released by bleaching gel

Testing endodontic sealers on human umbilical vein endothelial cells

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