Cytotoxicity of the Ascidian Cystodytes dellechiajei Against Tumor Cells and Study of the Involvement of Associated Microbiota in the Production of Cytotoxic Compounds

Abstract

Many cytotoxic compounds of therapeutic interest have been isolated from marine invertebrates, and some of them have been reported to be of microbial origin. Pyridoacridine alkaloids are the main compounds extracted from the ascidian Cystodytes dellechiajei. Here we describe the in vitro antiproliferative activity against different tumor cell lines of the ascidian extracts and provide some insights on the role of the microbial community associated with the tunicate in the production of these compounds. C. dellechiajei extracts showed remarkably high antiproliferative activity (IC 50 ≤5 µg/mL) in human lung carcinoma A-549, colon adenocarcinoma H-116, pancreatic adenocarcinoma PSN-1 and breast carcinoma SKBR3 cell lines. Moreover, we found that the maximum activity was located in the tunic tissue of the colony, which harbours a microbial community. In order to ascertain the involvement of this community in the synthesis of the bioactive compounds different approachs that included culture and culture independent methods were carried out. We undertook a screening for antiproliferative activities of the bacterial isolates from the ascidian, as well as a comprative analysis of the cytotoxic activities and the microbial communities from two color morphs of the ascidian, green and blue. In addition, the changes of the antiproliferative activities and the composition of the microbial communities were studied from ascidians kept in aquaria and treated with antibiotics for one month. Our

Characteristics of Sulfobacin A from a Soil Isolate Chryseobacterium gleum

“…9 Antiproliferative activity of the sulfobacin A from C. gleum LMG P-22264 Fig. 8 Inhibition of sulfobacin A from C. gleum LMG P-22264 biosynthesis by L-cycloserine adenocarcinoma H-116, pancreatic adenocarcinoma PSN-1, and breast carcinoma SKBR3 cell lines [27]. Cytoplasm fraction of lactic acid bacteria exhibited marked direct antiproliferative activities against colon and gastric cancer cell lines, whereas peptidoglycan was effective for the retardation of colon and bladder cancer cell lines [28].…”