Cytotoxicity of various types of gold-mesoporous silica nanoparticles in human breast cancer cells

Liu, Guomu; Li, Qiongshu; Ni, Weihua; Zhang, Nannan; Zheng, Xiao; Wang, Yingshuai; Shao, Dan; Tai, Guihua

doi:10.2147/ijn.s90887

Cited by 21 publications

(9 citation statements)

References 42 publications

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“…Silver and gold nanoparticles were reported to have an intrinsic property that allows a Fenton-type reaction to produce ROS [ 21 , 22 ] with a subsequent lipid and protein oxidation, DNA damage, and cell death induction [ 23 ]. Several studies underscored a direct interaction between the ability of NPs to initiate ROS production and the subsequent cell death [ 2 , 23 ]. Our results are consistent with this paradigm showing that administration of NPs resulted in oxidative stress induction in primary tumor tissue as was evidenced by accumulation of protein oxidation markers.…”

Section: Resultsmentioning

confidence: 99%

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Antitumor Activity of Alloy and Core-Shell-Type Bimetallic AgAu Nanoparticles

et al. 2017

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Nanoparticles (NPs) of noble metals, namely gold and silver, remain promising anticancer agents capable of enhancing current surgery- and chemotherapeutic-based approaches in cancer treatment. Bimetallic AgAu composition can be used as a more effective agent due to the synergetic effect. Among the physicochemical parameters affecting gold and silver nanoparticle biological activity, a primary concern relates to their size, shape, composition, charge, etc. However, the impact of metal components/composition as well as metal topological distribution within NPs is incompletely characterized and remains to be further elucidated and clarified. In the present work, we tested a series of colloidal solutions of AgAu NPs of alloy and core-shell type for an antitumor activity depending on metal molar ratios (Ag:Au = 1:1; 1:3; 3:1) and topological distribution of gold and silver within NPs (AucoreAgshell; AgcoreAushell). The efficacy at which an administration of the gold and silver NPs inhibits mouse Lewis lung carcinoma (LLC) growth in vivo was compared. The data suggest that in vivo antitumor activity of the studied NPs strongly depends on gold and silver interaction arising from their ordered topological distribution. NPs with Ag core covered by Au shell were the most effective among the NPs tested towards LLC tumor growth and metastasizing inhibition. Our data show that among the NPs tested in this study, AgcoreAushell NPs may serve as a suitable anticancerous prototype.

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Section: Resultsmentioning

confidence: 99%

“…Rapid advances in nanomedicine have generated an increasing number of potential diagnostic and therapeutic applications of nanoparticles in recent years [ 1 , 2 ]. Among the different types of nanoparticles, metal nanoparticles including gold and silver have widespread use across the biomedical sciences [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Antitumor Activity of Alloy and Core-Shell-Type Bimetallic AgAu Nanoparticles

et al. 2017

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“…AuNP were detected by reflection imaging by using 488 nm excitation, 481–493 nm emission, and DiI cell membrane dye was detected with 561 nm excitation, 563–612 nm emission wavelength. Images were illustrated using Fiji Image J software (Liu et al, 2015 ; Senthilkumar et al, 2015 ).…”

Section: Methodsmentioning

confidence: 99%

Stimuli-Responsive, Plasmonic Nanogel for Dual Delivery of Curcumin and Photothermal Therapy for Cancer Treatment

et al. 2021

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Nanogels (Ng) are crosslinked polymer-based hydrogel nanoparticles considered to be next-generation drug delivery systems due to their superior properties, including high drug loading capacity, low toxicity, and stimuli responsiveness. In this study, dually thermo-pH-responsive plasmonic nanogel (AuNP@Ng) was synthesized by grafting poly (N-isopropyl acrylamide) (PNIPAM) to chitosan (CS) in the presence of a chemical crosslinker to serve as a drug carrier system. The nanogel was further incorporated with gold nanoparticles (AuNP) to provide simultaneous drug delivery and photothermal therapy (PTT). Curcumin's (Cur) low water solubility and low bioavailability are the biggest obstacles to effective use of curcumin for anticancer therapy, and these obstacles can be overcome by utilizing an efficient delivery system. Therefore, curcumin was chosen as a model drug to be loaded into the nanogel for enhancing the anticancer efficiency, and further, its therapeutic efficiency was enhanced by PTT of the formulated AuNP@Ng. Thorough characterization of Ng based on CS and PNIPAM was conducted to confirm successful synthesis. Furthermore, photothermal properties and swelling ratio of fabricated nanoparticles were evaluated. Morphology and size measurements of nanogel were determined by transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). Nanogel was found to have a hydrodynamic size of ~167 nm and exhibited sustained release of curcumin up to 72 h with dual thermo-pH responsive drug release behavior, as examined under different temperature and pH conditions. Cytocompatibility of plasmonic nanogel was evaluated on MDA-MB-231 human breast cancer and non-tumorigenic MCF 10A cell lines, and the findings indicated the nanogel formulation to be cytocompatible. Nanoparticle uptake studies showed high internalization of nanoparticles in cancer cells when compared with non-tumorigenic cells and confocal microscopy further demonstrated that AuNP@Ng were internalized into the MDA-MB-231 cancer cells via endosomal route. In vitro cytotoxicity studies revealed dose-dependent and time-dependent drug delivery of curcumin loaded AuNP@Ng/Cur. Furthermore, the developed nanoparticles showed an improved chemotherapy efficacy when irradiated with near-infrared (NIR) laser (808 nm) in vitro. This work revealed that synthesized plasmonic nanogel loaded with curcumin (AuNP@Ng/Cur) can act as stimuli-responsive nanocarriers, having potential for dual therapy i.e., delivery of hydrophobic drug and photothermal therapy.

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“…The incorporation of AuNPs into silica shells to generate such systems was first achieved by functionalisation of their surface using a binary mixture of ligands, generating a vitreophylic face over which silica grows [26,27,28,29,30]. Janus Au@SiO 2 NPs can also be prepared by following a synthetic route that allows the growth of mesoporous instead of amorphous silica by using alcohols as solvents or co-solvents and controlling the addition rate of the precursor tetraethyl orthosilicate (TEOS) [31,32,33,34], by realising Pickering emulsions using melted paraffin wax [12,35], or by anchoring functionalised AuNPs to a glass or silicon substrate over which the silica grows [36].…”

Section: Introductionmentioning

confidence: 99%

“…As mentioned above, both traditional silica-coated and Janus NPs have been reported using MCM-41 [12,31,32,33,34,35,36]. However, the previous studies employed methodologies that rely on the use of binary solvent mixtures and varied concentrations of CTAB and silica sources, with reproducibility limited to specific nanoparticle (MCM-41 NP) sizes.…”

Section: Introductionmentioning

confidence: 99%

Facile Strategy for the Synthesis of Gold@Silica Hybrid Nanoparticles with Controlled Porosity and Janus Morphology

2019

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Hybrid materials prepared by encapsulation of plasmonic nanoparticles in porous silica systems are of increasing interest due to their high chemical stability and applications in optics, catalysis and biological sensing. Particularly promising is the possibility of obtaining gold@silica nanoparticles (Au@SiO2 NPs) with Janus morphology, as the induced anisotropy can be further exploited to achieve selectivity and directionality in physical interactions and chemical reactivity. However, current methods to realise such systems rely on the use of complex procedures based on binary solvent mixtures and varying concentrations of precursors and reaction conditions, with reproducibility limited to specific Au@SiO2 NP types. Here, we report a simple one-pot protocol leading to controlled crystallinity, pore order, monodispersity, and position of gold nanoparticles (AuNPs) within mesoporous silica by the simple addition of a small amount of sodium silicate. Using a fully water-based strategy and constant content of synthetic precursors, cetyl trimethylammonium bromide (CTAB) and tetraethyl orthosilicate (TEOS), we prepared a series of four silica systems: (A) without added silicate, (B) with added silicate, (C) with AuNPs and without added silicate, and (D) with AuNPs and with added silicate. The obtained samples were characterised by transmission electron microscopy (TEM), small angle X-ray scattering (SAXS), and UV-visible spectroscopy, and kinetic studies were carried out by monitoring the growth of the silica samples at different stages of the reaction: 1, 10, 15, 30 and 120 min. The analysis shows that the addition of sodium silicate in system B induces slower MCM-41 nanoparticle (MCM-41 NP) growth, with consequent higher crystallinity and better-defined hexagonal columnar porosity than those in system A. When the synthesis was carried out in the presence of CTAB-capped AuNPs, two different outcomes were obtained: without added silicate, isotropic mesoporous silica with AuNPs located at the centre and radial pore order (C), whereas the addition of silicate produced Janus-type Au@SiO2 NPs (D) in the form of MCM-41 and AuNPs positioned at the silica–water interface. Our method was nicely reproducible with gold nanospheres of different sizes (10, 30, and 68 nm diameter) and gold nanorods (55 × 19 nm), proving to be the simplest and most versatile method to date for the realisation of Janus-type systems based on MCM-41-coated plasmonic nanoparticles.

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Cytotoxicity of various types of gold-mesoporous silica nanoparticles in human breast cancer cells

Cited by 21 publications

References 42 publications

Antitumor Activity of Alloy and Core-Shell-Type Bimetallic AgAu Nanoparticles

Antitumor Activity of Alloy and Core-Shell-Type Bimetallic AgAu Nanoparticles

Stimuli-Responsive, Plasmonic Nanogel for Dual Delivery of Curcumin and Photothermal Therapy for Cancer Treatment

Facile Strategy for the Synthesis of Gold@Silica Hybrid Nanoparticles with Controlled Porosity and Janus Morphology

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