Cytotoxicity Studies of Curcumin Loaded-cockle Shell-derived Calcium Carbonate Nanoparticles

Abstract

Background: Cockle shell-derived calcium carbonate nanoparticles (CSCaCO3NP) are natural biogenic inorganic material that is used in drug delivery mainly as a bone-remodeling agent as well as a delivery agent for various therapeutics against bone diseases. Curcumin possess wide safety margin and yet puzzled with the problem of poor bioavailability due to insolubility. Propounding in vitro and in vivo studies on toxicity assessments of newly synthesized nanoparticles are ongoing to overcome some crucial challenges regarding their safety administration. Nanotoxicology has paved ways for concise test protocols to monitor sequential events with regards to possible toxicity of newly synthesized nanomaterials. The development of nanoparticle with no or less toxic effect has gained tremendous attentions. Objective: This study aimed at evaluating the in vitro cytotoxic effect of curcumin-loaded cockle shell-derived calcium carbonate nanoparticles (Cur-CSCaCO3NP) and assessing its biocompatibility on normal cells using standard techniques of WST’s assay. Method: Standard techniques of WST’s assay was used for the evaluation of the biocompatibility and cytotoxicity. Result: The result showed that CSCaCO3NP and Cur-CSCaCO3NP possess minimal toxicity and high biocompatibility on normal cells even at higher dose of 500 µg/ml and 40 µg/ml respectively. Conclusion: CSCaCO3NP can be termed an excellent non-toxic nanocarrier for curcumin delivery. Hence, curcumin loaded cockle shell derived calcium carbonate nanoparticles (Cur-CSCaCO3NP) could further be assessed for various in vivo and in vitro therapeutic applications against various bone related ailments.

Tuning polymorphs of precipitated calcium carbonate from discarded eggshells: effects of polyelectrolyte and salt concentration

“…For instance, calcite in eggshells, vaterite in fish otoliths and aragonite in mollusc shells. 4–7 The application of eggshell particles processed by simple mechanochemical methods has been extensively studied so far in different fields such as filler in polymers, 8–10 wastewater purification, 11–13 drug delivery and biomedical application. 14,15 The mechanochemical methods refer to the process of obtaining microparticles by applying mechanical energy at ambient temperature, such as grinding, ball milling, or mortar and pestle techniques.…”

Efficient Delivery of Recombinant Human Bone Morphogenetic Protein (Rhbmp-2) With Cockle Shell Derived Calcium Carbonate Nanoparticles (CaCO3NPs)

“…The approach suggested by (Hamidu et al, 2019, andMailafiya et al, 2021) was adopted with some changes to load CaCO3NPs (27,28). A mixture was created using 1 mg rhBMP-2, 15 mg CaCO3NPs, and 3 ml deionised water.…”

Evaluation of the chemical, physical, and biological properties of a newly developed bioceramic cement derived from cockle shells: an in vitro study

“…Cockle shells have a high calcium carbonate content [ 9 ]. These shells are abundantly available at a minimal cost, and in vitro studies have demonstrated that the calcium carbonate derived from cockle shells is biocompatible [ 10 ]. Nanoparticles derived from cockle shells have been used in medicine as a drug carrier targeting cancer cells [ 11 ].…”