Czapek-Dox agar containing iprodione and dicloran as a selective medium for the isolation of Fusarium species

Abildgren, M. P.; Lund, Flemming; Thrane, Ulf; Elmholt, Susanne

doi:10.1111/j.1472-765x.1987.tb01620.x

Cited by 119 publications

(51 citation statements)

References 5 publications

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“…Monitoring of fungal viability in soil in treatment 4 was done by taking 1 g samples of soil from a layer 1-2 cm under the soil surface. Samples were diluted in 50 ml SDW, filtered through sterile cheese-cloth, and 500 μl were spread on Czapek-Dox iprodione dichloran (CZID) agar (Abildgren et al 1987). Plates were incubated for 1 week in dark at room temperature, upon which the number of white fungal colonies was counted.…”

Section: Re-isolation Of F Langsethiaementioning

confidence: 99%

Fusarium langsethiae (Torp and Nirenberg), investigation of alternative infection routes in oats

et al. 2011

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Fusarium langsethiae is a recently characterized fungus within the genus Fusarium. It is found as a grain contaminant of small grain cereals such as oats and barley, and to a lesser extent wheat. Fusarium langsethiae is particularly widespread in the Nordic countries and the UK where it poses a serious problem as the main producer of T-2 and HT-2 mycotoxins. The biology of F. langsethiae and its interaction with the plant remains poorly understood, partly hampered by difficulties reproducing a natural level of infection under controlled conditions. The reported study was designed as a series of glasshouse experiments to advance our understanding of F. langsethiae biology by investigating alternative infection routes and its proliferation in oats, Avena sativa. Various methods of seed, soil, and seedling inoculation, boot injection and spray inoculation, were tested. The results clearly show a strong preference of F. langsethiae for the panicle, ruling out alternative infection routes. At relatively low temperatures spray infection, accompanied by prolonged humidity, ensured a thorough establishment of the fungus both at flowering and at early dough stage. Boot injection proved to be a reliable working tool for production of an even and predictable grain infection. Apart from in the panicle, considerable fungal proliferation was only detected in flag leaf nodes, and was a direct consequence of the boot injection method. Fungal presence in the node tissue also correlated with significant stunting of infected shoots. In light of the results the pathogenic and endophytic abilities of F. langsethiae are discussed.

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Section: Re-isolation Of F Langsethiaementioning

confidence: 99%

Fusarium langsethiae (Torp and Nirenberg), investigation of alternative infection routes in oats

et al. 2011

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“…Kernels of each sample were surface sterilized for two minutes in 1.3% aqueous sodium hypochlorite, rinsed twice with sterile distilled water and transferred onto Czapek-Dox-Iprodion-Dichloran (CZID) agar (Abildgren et al 1987). After incubation at 23°C for 6 days, the percentage of infected kernels was recorded.…”

Section: Mycological Analysismentioning

confidence: 99%

Fusarium species and mycotoxin profiles on commercial maize hybrids in Germany

Goertz¹,

et al. 2010

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High year-to-year variability in the incidence of Fusarium spp. and mycotoxin contamination was observed in a two-year survey investigating the impact of maize ear rot in 84 field samples from Germany. Fusarium verticillioides, F. graminearum, and F. proliferatum were the predominant species infecting maize kernels in 2006, whereas in 2007 the most frequently isolated species were F. graminearum, F. cerealis and F. subglutinans. Fourteen Fusariumrelated mycotoxins were detected as contaminants of maize kernels analyzed by a multi-mycotoxin determination method. In 2006, a growth season characterized by high temperature and low rainfall during anthesis and early grain filling, 75% of the maize samples were contaminated with deoxynivalenol, 34% with fumonisins and 27% with zearalenone. In 2007, characterized by moderate temperatures and frequent rainfall during the entire growth season, none of the 40 maize samples had quantifiable levels of fumonisins while deoxynivalenol and zearalenone were detected in 90% and 93% of the fields, respectively. In addition, 3-acetyldeoxynivalenol, 15-acetyldeoxnivalenol, moniliformin, beauvericin, nivalenol and enniatin B were detected as common contaminants produced in both growing seasons. The results demonstrate a significant mycotoxin contamination associated with maize ear rots in Germany and indicate, with regard to anticipated climate change, that fumonisins-producing species already present in German maize production may become more important.

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“…For isolation of Fusarium species, wheat kernels were surface-sterilized using NaOCl (1.3% available chlorine) for 2 min and placed into Petri dishes (5 kernels per dish) containing Czapek-iprodione-dicloran agar (CZID-agar) prepared according to Abildgren et al (1987). After incubation at 22°C for 7 days under near-UV light mycelium growing from the kernels was transferred onto SNA (Nirenberg 1976) and PDA and grown at 22°C for 7 days.…”

Section: Isolation and Identification Of Fungimentioning

confidence: 99%

Effect of fungicides on the complex of Fusarium species and saprophytic fungi colonizing wheat kernels

et al. 2007

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Fusarium head blight of wheat, often associated with mycotoxin contamination of food and feed is caused by various Fusarium species. The efficacy of fungicide sprays for the control of the disease and mycotoxins varies from being highly effective to even increasing mycotoxin levels. The potential role of antagonistic fungi in this variability was investigated assessing sensitivity of Fusarium species and saprophytic fungi colonizing wheat kernels to fungicides. Saprophytes were tested for their antagonistic activity to the prevalent Fusarium species Fusarium avenaceum, Fusarium culmorum, Fusarium graminearum, and Fusarium poae. Fungal isolates from mature winter wheat kernels were Alternaria alternata, Arthrinium sp., Aspergillus niger, Epicoccum sp., Microdochium spp., Rhizopus oryzae and Trichoderma sp. In dual culture A. niger, R. oryzae and Trichoderma hamatum were more effective in reducing mycelial growth of Fusarium species than Microdochium majus; A. alternata and Epicoccum sp. were ineffective because of slow growth rates. Saprophytic fungi were sensitive to triazoles; however, prothioconazole and tebuconazole had stronger effects on mycelial growth of Fusarium species. ED 50 values also indicated significant differences in the sensitivity of Fusarium species to triazoles (range 0.1-1.7 mg l −1 ). Azoxystrobin and fluoxastrobin were largely ineffective in inhibiting in vitro growth of Fusarium spp.; sensitivity of the other fungi was generally lower, except for M. majus which was highly sensitive. Due to differences in fungicide sensitivity among Fusarium spp. and ear-colonizing fungi antagonistic to Fusarium spp. fungicides are likely to modify the balance within the mycoflora of wheat ears which may also affect the mycotoxin contamination of grain.

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Czapek-Dox agar containing iprodione and dicloran as a selective medium for the isolation of Fusarium species

Cited by 119 publications

References 5 publications

Fusarium langsethiae (Torp and Nirenberg), investigation of alternative infection routes in oats

Fusarium langsethiae (Torp and Nirenberg), investigation of alternative infection routes in oats

Fusarium species and mycotoxin profiles on commercial maize hybrids in Germany

Effect of fungicides on the complex of Fusarium species and saprophytic fungi colonizing wheat kernels

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