d- and l-amino acids in Antarctic lakes: assessment of a very sensitive HPLC-MS method

Barbaro, Elena; Zangrando, Roberta; Vecchiato, Marco; Turetta, Clara; Barbante, Carlo; Gambaro, Andrea

doi:10.1007/s00216-014-7961-y

Cited by 32 publications

(17 citation statements)

References 60 publications

(80 reference statements)

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“…The enantiomeric determination of free L-and D-amino acids by HPLC-MS/MS has been described in detail by Barbaro et al (2014). This instrumental method has been applied to the aqueous extracts of the aerosol samples collected during this study.…”

Section: Instrumental Analysismentioning

confidence: 99%

“…For other amino acids, where a labeled standard was unavailable, an internal standard was used to quantify the analytes. A detailed description of which analytes are quantified with which method can be found in Barbaro et al (2014). In both cases, the results were corrected for daily instrumental sensitivity variations by evaluating the instrumental response factors.…”

Section: Instrumental Analysismentioning

confidence: 99%

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Free amino acids in Antarctic aerosol: potential markers for the evolution and fate of marine aerosol

et al. 2015

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Abstract. To investigate the impact of marine aerosols on global climate change it is important to study their chemical composition and size distribution. Amino acids are a component of the organic nitrogen in aerosols and particles containing amino acids have been found to be efficient ice nuclei. The main aim of this study was to investigate the L- and D-free amino acid composition as possible tracers of primary biological production in Antarctic aerosols from three different areas: two continental bases, Mario Zucchelli Station (MZS) on the coast of the Ross Sea, Concordia Station at Dome C on the Antarctic Plateau, and the Southern Ocean near the Antarctic continent. Studying the size distribution of amino acids in aerosols allowed us to characterize this component of the water-soluble organic carbon (WSOC) in marine aerosols near their source and after long-range transport. The presence of only free L-amino acids in our samples is indicative of the prevalence of phytoplanktonic material. Sampling at these three points allowed us to study the reactivity of these compounds during long-range transport. The mean total amino acid concentration detected at MZS was 11 pmol m−3, a higher percentage of amino acids were found in the fine fraction. The aerosol samples collected at Dome C had the lowest amino acid values (0.7 and 0.8 pmol m−3), and the coarse particles were found to have higher concentrations of amino acids compared to the coastal site. The amino acid composition in the aerosol collected at Dome C had also changed compared to the coastal site, suggesting that physical and chemical transformations had occurred during long range transport. During the sampling cruise on the R/V Italica on the Southern Ocean, high concentrations of amino acids were found in the total suspended particles, this we attribute to the presence of intact biological material (as microorganisms or plant material) in the sample.

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Section: Instrumental Analysismentioning

confidence: 99%

Section: Instrumental Analysismentioning

confidence: 99%

Free amino acids in Antarctic aerosol: potential markers for the evolution and fate of marine aerosol

et al. 2015

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“…Quantifications of the AA were obtained using highperformance liquid chromatography with a chiral column coupled with tandem mass spectrometry for detection (HPLC-MS/ MS) (Barbaro et al, 2014). PC were analyzed using reverse-phase chromatography coupled with tandem mass spectrometry (Zangrando et al, 2013), whereas sugars were determined using ion chromatography coupled with mass spectrometry (IC-MS) (Barbaro et al, 2015a).…”

Section: Instrumental Analysismentioning

confidence: 99%

Aerosol and snow transfer processes: An investigation on the behavior of water-soluble organic compounds and ionic species

et al. 2017

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h i g h l i g h t sThe air-snow transfer processes were evaluated using water soluble compounds. This is the first study about amino acids and sugar concentration in surface snow. Water soluble organic fractions of Antarctic aerosol and snow were investigated. a r t i c l e i n f o b s t r a c tThe concentrations of water-soluble compounds (ions, carboxylic acids, amino acids, sugars, phenolic compounds) in aerosol and snow have been determined at the coastal Italian base "Mario Zucchelli" (Antarctica) during the 2014e2015 austral summer. The main aim of this research was to investigate the air-snow transfer processes of a number of classes of chemical compounds and investigate their potential as tracers for specific sources.The composition and particle size distribution of Antarctic aerosol was measured, and water-soluble compounds accounted for 66% of the PM 10 total mass concentration. The major ions Na þ , Mg 2þ , Cl À and SO 4 2À made up 99% of the total water soluble compound concentration indicating that sea spray input was the main source of aerosol. These ionic species were found mainly in the coarse fraction of the aerosol resulting in enhanced deposition, as reflected by the snow composition. Biogenic sources were identified using chemical markers such as carboxylic acids, amino acids, sugars and phenolic compounds. This study describes the first characterization of amino acids and sugar concentrations in surface snow. High concentrations of amino acids were found after a snowfall event, their presence is probably due to the degradation of biological material scavenged during the snow event. Alcohol sugars increased in concentration after the snow event, suggesting a deposition of primary biological particles, such as airborne fungal spores.

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“…Indeed, peptidoglycan in microbial cellular walls contains a high proportion of D-Ala, D-Asp, D-Glu and D-Ser (Dittmar et al, 2001;McCarthy et al, 1998). Due to their low volatility, AAs have been observed in condensed phases such as particulate matter (Barbaro et al, 2011;Barbaro et al, 2015;Di Filippo et al, 2014;Matsumoto and Uematsu, 2005;Scalabrin et al, 2012), dew (Scheller, 2001), rain (Mace et al, 2003b;Mace et al, 2003c), fog (Zhang and Anastasio, 2003), microlayer (Kuznetsova et al, 2004;Matrai et al, 2008), lakes (Barbaro et al, 2014) and marine waters (Kuznetsova et al, 2004;Matrai et al, 2008;Sommerville and Preston, 2001).…”

Section: (And References Therein)mentioning

confidence: 99%

Five primary sources of organic aerosols in the urban atmosphere of Belgrade (Serbia)

Zangrando

Barbaro

Kirchgeorg

et al. 2016

Science of The Total Environment

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Biomass burning and primary biological aerosol particles (PBAPs) represent important primary sources of organic compounds in the atmosphere. These particles and compounds are able to affect climate and human health. In the present work, using HPLC-orbitrapMS, we determined the atmospheric concentrations of molecular markers such as anhydrosugars and phenolic compounds that are specific for biomass burning, as well as the concentrations of sugars, alcohol sugars and D- and L-amino acids (D-AAs and L-AAs) for studying PBAPs in Belgrade (Serbia) aerosols collected in September–December 2008. In these samples, high levels of all these biomarkers were observed in October. Relative percentages of vanillic (V), syringic compounds (S) and p-coumaric acid (PA), as well as levoglucosan/mannosan (L/M) ratios, helped us discriminate between open fire events and wood combustion for domestic heating during the winter. L-AAs and D-AAs (1% of the total) were observed in Belgrade aerosols mainly in September–October. During open fire events, mean D-AA/L-AA (D/L) ratio values of aspartic acid, threonine, phenylalanine, alanine were significantly higher than mean D/L values of samples unaffected by open fire. High levels of AAs were observed for open biomass burning events. Thanks to four different statistical approaches, we demonstrated that Belgrade aerosols are affected by five sources: a natural source, a source related to fungi spores and degraded material and three other sources linked to biomass burning: biomass combustion in open fields, the combustion of grass and agricultural waste and the combustion of biomass in stoves and industrial plants. The approach employed in this work, involving the determination of specific organic tracers and statistical analysis, proved useful to discriminate among different types of biomass burning events

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d- and l-amino acids in Antarctic lakes: assessment of a very sensitive HPLC-MS method

Cited by 32 publications

References 60 publications

Free amino acids in Antarctic aerosol: potential markers for the evolution and fate of marine aerosol

Free amino acids in Antarctic aerosol: potential markers for the evolution and fate of marine aerosol

Aerosol and snow transfer processes: An investigation on the behavior of water-soluble organic compounds and ionic species

Five primary sources of organic aerosols in the urban atmosphere of Belgrade (Serbia)

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