d-Fructose

Klotzsch, Helmut; Bergmeyer, Hans‐Ulrich

doi:10.1016/b978-0-12-395630-9.50030-x

Cited by 15 publications

(13 citation statements)

References 5 publications

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“…Sucrose was measured as described by Bergmeyer and Klotzsch (1963); the reaction mixture contained initially 0.13 mM citrate buffer, pH 4.6, and 40 units of fl-fructosidase in a total volume of 0.3 ml. Incubation was performed for 15 min at 25"C and the pH of the solution was brought to about 7.6 by the addition of 50/zl of 1 M Tris base and 150/zl of 0.5 M Tris-HC1 of pH 7.6.…”

Section: Methodsmentioning

confidence: 99%

“…The reaction was started by the addition of HK. Glucose and fructose were measured enzymatically by a coupled-enzyme assay, using HK, as described by Slein (1963) and by Klotzsch and Bergmeyer (1963); the reaction mixture contained 50 mM Tris-HC1, pH 7.6, 4 mM MgC12, 0.4 mM NADP § 2 mM ATP, 0.7 unit of G6PDH, and 7 units of HK in a total volume of 1.5 ml. The reaction was started by the addition of HK (glucose assay), and after completion of the dehydrogenase reaction, fructose 6-phosphate was measured after the addition of 2.8 units of phosphoglucose isomerase (fructose assay).…”

Section: Methodsmentioning

confidence: 99%

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Relationship between wintering ability of winter wheat and the extent of depression of carbohydrate reserves: Basal metabolic rate under snow determines longevity of plants

Sagisaka

Matsuda

Okuda

et al. 1991

Soil Science and Plant Nutrition

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Relationship between wintering ability of winter wheat and the extent of depression of carbohydrate reserves: Basal metabolic rate under snow determines longevity of plants

Sagisaka

Matsuda

Okuda

et al. 1991

Soil Science and Plant Nutrition

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“…Accordingly, in both the patients with HFI exposed to fructose, and in the fructose-loaded rat, the depletion of Pi induced in the renal cortex, and hence the disturbance of adenine nucleotide metabolism in this tissue, might be both less severe than that induced in the liver and more susceptible to attenuation by phosphate loading, and by adenosine loading (vide infra). The 40 ,umol/g body weight. Control rats were anesthetized only.…”

Section: Introductionmentioning

confidence: 99%

Evidence that the Severity of Depletion of Inorganic Phosphate Determines the Severity of the Disturbance of Adenine Nucleotide Metabolism in the Liver and Renal Cortex of the Fructose-Loaded Rat

Morris¹,

Nigon²,

Reed³

1978

J. Clin. Invest.

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A B S T R A C T To test the hypothesis that in both the liver and renal cortex ofthe fructose-loaded rat, severity of depletion of inorganic phosphate (Pi), and not the magnitude of accumulation of fructose-l-phosphate (F-1-P), determines the severity of the dose-dependent reduction of ATP, we intraperitoneally injected fed rats with fructose, 20 and 40 umol/g, alone, and at the higher load, in combination with (a) sodium phosphate, 20 umol/g, administered shortly beforehand or subsequently or, (b) adenosine, 2 ,umol/g, administered beforehand. The following observations were made: (a) With fructose loading alone, at the higher load, both Pi and total adenine nucleotides (TAN) were reduced by one third in the renal cortex and (as previously observed) by two thirds in the liver; and at either load, the reduction of ATP (and TAN)

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“…When sucrose was present in the sample, the enzyme preparation (PGO) was dissolved in lO 6 m AgNOa, which inhibited the invertase activity of the preparation. Glucose was also determined with hexokinase, glucose-6-phosphate dehydrogenase method [Slein, 1956], Fructose was determined with hexokinase, glucose-6-phosphate dehydrogenase, phosphoglucoisomerase method [K lotzsch and Bergmeyer, 1965).…”

Section: Methodsmentioning

confidence: 99%

A Levansucrase from Streptococcus mutans

Carlsson¹

1970

Caries Res

109

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One of the predominant bacteria in early dental plaque, Streptococcus mutans, has been shown to synthesize levansucrase. This enzyme was obtained from the supernatant fluid of a glucose-broth culture. Purification of the enzyme was achieved in two steps by adsorption on hydroxylapatite followed by isoelectric focusing and fractionation. This resulted in a 10% yield and a 200-fold purification. Sucrose as well as raffinose could serve as substrate for the enzyme. In addition to levan (MW > 25 × 10⁶), free fructose and oligosaccharides were formed from sucrose. The enzyme had a pH optimum at 6.0, optimal activity around 40° C and isoelectric point at pH 4.2. Iodoacetamide, cysteine, Tris and sodium fluoride in a concentration of 0.05 m had no effect on the enzyme activity. The enzyme was totally inhibited by 0.1 mM Hg⁺⁺ and to about 40% by 1 mM Pb⁺⁺, Ag⁺, Ni⁺⁺ and Cu⁺⁺. EDTA in a concentration of 1 mM reduced the over-all activity of the enzyme by 75% and specifically interfered with the synthesis of levan. Most divalent cations could restore the activity of the enzyme in the presence of EDTA. The properties of this streptococcal levansucrase were similar to those previously described for levansucrases from Aerobacter levanicum and Bacillus subtilis. The finding, that EDTA interfered with the synthesis of levan, may give new possibilities in elucidating the mechanism of this enzyme reaction.

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d-Fructose

Cited by 15 publications

References 5 publications

Relationship between wintering ability of winter wheat and the extent of depression of carbohydrate reserves: Basal metabolic rate under snow determines longevity of plants

Relationship between wintering ability of winter wheat and the extent of depression of carbohydrate reserves: Basal metabolic rate under snow determines longevity of plants

Evidence that the Severity of Depletion of Inorganic Phosphate Determines the Severity of the Disturbance of Adenine Nucleotide Metabolism in the Liver and Renal Cortex of the Fructose-Loaded Rat

A Levansucrase from Streptococcus mutans

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