D-Glucose: Preferential Binding to Brush Borders Disrupted with Tris(hydroxymethyl)aminomethane

Faust, Robert G.; Wu, Shih-Min Liu; Faggard, Mary L.

doi:10.1126/science.155.3767.1261

Cited by 24 publications

(9 citation statements)

References 10 publications

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“…The results of these studies support our previous work (8) in which we suggested the possibility that preferential D-glucose binding to Tris-disrupted brush borders is related to the initial step in active sugar transport by the small intestine. We have shown that only sugars which are actively transported by the small intestine inhibit preferential D-glucose binding, presumably by competing for similar sites on a "carrier" or a component within the brush border membrane of mucosal cells.…”

Section: Discussionsupporting

confidence: 91%

“…The methods employed to isolate the epithelial brush border membranes from hamster jejunum were the same as those previously reported (8) and yielded a substantially pure preparation as is shown in Fig. 1.…”

Section: Isolation and Preparation Of Brush Bordersmentioning

confidence: 99%

“…Recently, Faust et al (8) reported that brush borders isolated from the mucosa of hamster jejunum and disrupted with tris (hydroxymethyl) aminomethane (9) can preferentially bind actively transported D-glucose. Preferential binding of D-glucose was demonstrated by an increase in the disintegrations per minute (dpm) ratio of D-mannose-3H to D-glucose-14C in the supernatant from incubated Tris-disrupted brush borders.…”

Section: Introductionmentioning

confidence: 99%

“…It has been suggested that preferential D-glucose binding to Tris-disrupted brush borders is similar to the initial step in active sugar transport by the small intestine (8) in that this binding is not directly dependent upon energy derived from Na +-K+-stimulated, Mg2+-dependent ATPase activity, but is inhibited by: (a) phlorizin, a competitive inhibitor of active sugar transport, (b) mercuric chloride, a compound which binds sulfhydryl groups and denatures protein, and (c) a decrease in temperature, which is indicative of a temperature-dependent complexing between the sugar and the Tris-disrupted brush borders.…”

Section: Introductionmentioning

confidence: 99%

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Active Sugar Transport by the Small Intestine

Faust

Leadbetter

Plenge

et al. 1968

The Journal of General Physiology

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The effects of sugars, amino acids, hexosamines, sulfhydryl-reacting compounds, and cations on the p@rential binding of D-glucose to Tris-disrupted brush borders ABSTRACT Tris-disrupted and intact brush border membrane preparations from mucosa of hamster jejunum were capable of preferentially binding actively transported D-glucose in a similar manner. Density gradient centrifugation of the Tris-disrupted brush borders indicated that D-glucose was bound to a fraction containing the cores or inner material of the microvilli. The properties of this binding were examined with the Tris-disrupted brush border preparation. Actively transported sugars competitively inhibited preferential D-glucose binding, whereas no effect was observed with nonactively transported sugars. Neither actively nor nonactively transported amino acids affected D-glucose binding. D-Glucosamine, which is not actively transported, was inhibitory to preferential D-glucose binding as well as to the active transport of D-glucose by everted sacs of hamster jejunum. No inhibitory effect was observed with the same concentration of D-galactosamine. Preferential D-glucose binding was also inhibited by sulfhydryl-reacting compounds, Ca 2+, and Li + ions. On the other hand, Mg 2+ was shown to be stimulatory and Na +, N H +, and K + had no effect on this phenomenon. The results of these experiments suggest that preferential D-glucose binding to brush borders is related to the initial step in active sugar transport by the small intestine.

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Section: Discussionsupporting

confidence: 91%

Section: Isolation and Preparation Of Brush Bordersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Active Sugar Transport by the Small Intestine

Faust

Leadbetter

Plenge

et al. 1968

The Journal of General Physiology

Self Cite

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show abstract

“…Proteins have been suspected of being involved in other transport systems in animals but little is known of their nature. Transport of glucose by intestine (72,73) and by the erythrocyte (4,74,75), for example, have been related to binding by proteins or protein-like substances. Similarly, work is proceeding on the purification of a carrier-like protein for organic bases 8 that was released by osmotic shock from the kidney (76).…”

Section: T R a N S P O R T P R O T E I N Smentioning

confidence: 99%

Binding Proteins from Animals with Possible Transport Function

Wasserman

Corradino

Taylor

1969

The Journal of General Physiology

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Several proteins from various animal tissues with possible transport function have been briefly described, with emphasis given to a vitamin D-induced calcium-binding protein (CaBP) implicated in calcium translocation across epithelial membranes. The latter protein was shown to be present in the small intestine, colon, kidney, and the uterus (shell gland) of the chicken. CaBP was also found in the small intestine of the rat, dog, bovine, and monkey. This protein has been isolated in high purity from chick intestinal mucosa and some of its properties determined. Its molecular weight is about 28,000, its formation constant, about 2.6 x 105 M-1, and its binding capacity, 1 calcium atom per protein molecule. Correlative studies have shown that CaBP concentration in intestinal mucosa varies with the calcium absorptive capacity of the gut, thereby suggesting that CaBP is intimately involved in the process of calcium absorption. CaBP has been localized in the brush border region of the intestinal absorptive cell and within goblet cells. Among other proteins mentioned were the intrinsic factor required for vitamin B12 absorption and the protein(s) associated with iron translocation.

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Avian infectious bronchitis virus characteristics of an inhibitor found in serum

Lukert

1973

Archiv f Virusforschung

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D-Glucose: Preferential Binding to Brush Borders Disrupted with Tris(hydroxymethyl)aminomethane

Cited by 24 publications

References 10 publications

Active Sugar Transport by the Small Intestine

Active Sugar Transport by the Small Intestine

Binding Proteins from Animals with Possible Transport Function

Avian infectious bronchitis virus characteristics of an inhibitor found in serum

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