Chemical and isotopic compositions were analyzed on geothermal steam from fumaroles and wells in the Takinoue geothermal area. The distribution of deuterium and oxygen-18 in water samples sug gests that the fumarole steam discharge is formed from the rising geothermal fluid through a single-step steam separation process at temperatures of approximately 150-240°C. On the basis of N2/Ar, He/Ar and C02/N2 ratios, and 813C values of carbon dioxide in steam, it is concluded that the gas components dissolved in the Takinoue geothermal waters are mixtures of magmatic gases and meteoric waters.