d-Hydantoinase from anaerobic microorganisms

Abstract

Of 373 anaerobic microbial isolates screened for the enzymatic conversion of dihydrouracii to N-carbamyl-fl-alanine, several strains of Clostridium spp., C. glycolicum, C. subterminale and Peptococcus anaerobius were positive. These Clostridium and Peptococcus strains produced also N-carbamyl-D-amino acids from the respective 5'-monosubstituted hydantoins. The Dhydantoinase activity from whole cell suspensions of P. anaerobius strain C R D A 303 was characterized with regard to pH and temperature stability and activity by using dihydrouracil (DHU) and isopropylhydantoin (IPH) as substrates. The D-hydantoinase from P. anaerobius was optimal at 60°C and at pH 6.5-9.5 for the substrate DHU. It was stable up to 55°C and at pH 5.0-9.5 and could be stored at 4°C under an aerobic atmosphere for at least 14 days.

Purification and biochemical characterization of the hydantoin hydrolyzing enzyme from Agrobacterium species

“…In the modern literature, microbial hydantoinases are believed to be identical, similar, homologous or at least equivalent Morin et al, 1986;Moller et al, 1988;Morin et al, 1991) to the animal dihydropyrimidinase (5,6-dihydropyrimidine amidohydrolase), a key enzyme in the catabolism of uracil and thymine (Vogels and Van Der Drift, 1976). The concept of identity of the microbial hydantoinase with the dihydropyrimidinase originally stems from the work of Eadie et al (1949) and of Wallach and Grisolia (1 957) who showed that the dihydropyrimidinase isolated either from calf liver or from plant homogenates, catalyzed not only the hydrolysis of dihydrouracil and dihydrothymine, but also that of the structurally related 5-member cyclic hydantoin molecule.…”

Study on the Synthesis, Characterization and Bioactivities of 3-Methyl-9’-fluorenespiro-5-hydantoin

“…[36][37][38] and even from anaerobic microorganisms. 39 However, recently a hydantoinase from Agrobacterium was identified which exhibits no dihydropyrimididase activity. 34 DL-5-Monosubstituted hydantoins are converted to D-amino acids via N-carbamoyl-D-amino acids by some bacteria.…”