d-Lactate is not a reliable marker of gut ischemia–reperfusion in a rat model of supraceliac aortic clamping*

Collange, Olivier; Tamion, Fabienne; Chanel, Stéphane; Hue, G.; Richard, Vincent; Thuilliez, C.; Bertrand, Denis; Plissonnier, Didier

doi:10.1097/01.ccm.0000214517.24064.35

Cited by 29 publications

(18 citation statements)

References 22 publications

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“…An earlier model using supra-coeliac ischaemia (40 min)/reperfusion (1 h) was associated with higher intestinal injury (lactate 7.1 AE 1.6 mmol l À1 ). 32 One hour of ischaemia was associated with >50% lethality within the first hour of reperfusion (unpublished data). Our present model induced significant histological injury, apoptosis and an increase in lactate (3.3 AE 1 mmol l À1 ) but was not lethal, and is moreover the most widely used experimental model.…”

Section: Discussionmentioning

confidence: 95%

Methylene Blue Protects Liver Oxidative Capacity after Gut Ischaemia–Reperfusion in the Rat

Collange

Charles

Bouitbir

et al. 2013

European Journal of Vascular and Endovascular Surgery

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Section: Discussionmentioning

confidence: 95%

Methylene Blue Protects Liver Oxidative Capacity after Gut Ischaemia–Reperfusion in the Rat

Collange

Charles

Bouitbir

et al. 2013

European Journal of Vascular and Endovascular Surgery

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“…Most frequently encountered laboratory anomalies are leukocytosis and metabolic acidosis (7). Besides, there have been lotof standard enzyme studies including inorganic phosphate and creatinine kinase, lactate dehydrogenase, and aspartate aminotransferase in patients with suspected AMI (6,14,26). Since most of these tests are abnormal in these patients, none of these tests are specifi c and sensitive enough to make a diagnosis in early stages.…”

Section: Discussionmentioning

confidence: 99%

“…Even though serum amylase, aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) are elevated, none of these are sensitive or specifi c (7). There are a lot of studies investigating the serum levels of D-dimer, alpha-glutathion S-transferase, Dlactate, L-lactate, LDH, intestinal fatty acid binding protein, alkaline phosphatase and CPK for early diagnosis of AMI but there are controversial reports about the usage of these markers in early diagnosis of instestinal ischaemia (10)(11)(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

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The place of D-dimer and L-lactate levels in the early diagnosis of acute mesenteric ischemia

Aydın¹,

Ozban²,

Serinken³

et al. 2015

BLL

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INTRODUCTION: Acute mesenteric ischemia (AMI) is an abdominal-vascular emergency which is rare and has high mortality rates (60-80 %) due to late diagnosis (1-3). Although it is known that extravascular reasons like intestinal intussusception, volvulus, strangulated hernias and obstructions can cause intestinal gangrene, these are rarely the cause of AMI (1). MATERIALS AND METHODS: In this study, we used male Wistar-Albino rats weighing 250-300 grams obtained from Pamukkale University Experimental Research Laboratory. Animals were exposed to light-dark cycles for 12 hours and had free access to food and water. They were kept in cages for 7 days to stabilise their intestinal fl ora. In animals of group I, nothing was made other than taking 0.5 ml blood intracardially. In other animals, abdomen was reached with midline laparotomy and superior mesenteric artery (SMA) was located. In group II (operative control group), SMA was isolated and manipulated but was not ligated. In Group III (intestinal ischemia group), SMAwas isolated and ligated with 3/0 silk tie distally to the aorta. After this process, intestinal ischemia was achieved which was confi rmed by paleness and pulselessness of intestines, caecum and right colon. Later on, abdomen was closed with double 3/0 polyglactin sutures. At postoperative 1st, 4th and 6th hours 0.5 ml blood was taken intracardially from the animals in groups II and III in order to quantify D-dimer and L-lactate levels. LABORATORY TESTS: D-dimer: Blood samples which were put into tubes containing sodium citrate, were seperated from plasma with centrifugation at 4000 rpm for 7 minutes. L-lactate: Blood L-lactate levels were determined from blood taken into capillary tubes with the help of immobilised enzyme electrode technology using YSI 1500 Sport portative lactate analyzer (Yellow Springs Instruments Inc., Ohio-USA). HISTOPATHOLOGIC VERIFICATION: Two cm long intestinal samples were taken from animals in which SMA was ligated in order to achieve mesenteric ischemia and these samples were fi xed in 10 % formol. DISCUSSION: As a result, in rats with SMA occlusion serum D-dimer levels were not increased signifi cantly when compared either in the group or with the basal values of the control group and values in operative control group. Therefore, it is concluded that D-dimer is not a useful marker for early diagnosis of AMI. On the other hand, it is revealed that blood L-lactate levels began to increase signifi cantly following 4th hour of mesenteric ischemia and it is shown that this increase continued at the 6th hour. In addition, considering the utmost importance of the early diagnosis in patients with the clinical suspicion of AMI, L-lactate seems to be a suitable marker to use in emergency departments because it is achieved with a portable device that gives fast and accurate results. Nevertheless, our results are need to be supported by clinical studies with larger patient series (Tab. 2, Fig. 11, Ref. 39). Text in PDF www.elis.sk.

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“…[3] Markers such as D-dimer, alpha-glutathione, S-transferase, D-lactate, intestinal fatty acid binding protein (IFABP), alkaline phosphatase (ALP), procalcitonin and diamine oxidase (DAO) have been researched in this context; however, definitive results have not been reported. [3,7,8] Thus, research into the early diagnosis of AMI continues. Angiography can provide a certain diagnosis of AMI, having recent studies report that multi-detector angio-CT is as effective as angiography in the diagnosis of AMI.…”

mentioning

confidence: 99%

Multidetector Angio-CT and the Use of D-Dimer for the Diagnosis of Acute Mesenteric Ischemia in Geriatric Patients

Gün

Yolcu

Değerli

et al. 2014

Ulus Travma Acil Cerrahi Derg

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d-Lactate is not a reliable marker of gut ischemia–reperfusion in a rat model of supraceliac aortic clamping*

Abstract: D-lactate is not a reliable marker of gut IR in our model of supraceliac aortic clamping in rats.

Cited by 29 publications

References 22 publications

Methylene Blue Protects Liver Oxidative Capacity after Gut Ischaemia–Reperfusion in the Rat

Methylene Blue Protects Liver Oxidative Capacity after Gut Ischaemia–Reperfusion in the Rat

The place of D-dimer and L-lactate levels in the early diagnosis of acute mesenteric ischemia

Multidetector Angio-CT and the Use of D-Dimer for the Diagnosis of Acute Mesenteric Ischemia in Geriatric Patients

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