D-mannose-Coating of Maghemite Nanoparticles Improved Labeling of Neural Stem Cells and Allowed Their Visualization by <i>ex vivo</i> MRI after Transplantation in the Mouse Brain

Pongrac, I; Radmilović, Marina Dobrivojević; Ahmed, Lada Brkić; Mlinarić, Hrvoje; Regul, Jan; Škokić, Siniša; Babič, Michal; Horák, Daniel; Hoehn, Mathias; Gajović, Srećko

doi:10.1177/0963689719834304

Cited by 20 publications

(9 citation statements)

References 86 publications

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“…This ability opens up a new pathway for using SPIONs for ROS amplification in combination with anticancer drugs to enhance cancer treatment. PA-SPIONs generate continuous Fe 2+ /Fe 3+ release in cancer cells in acidic organelles such as endosomes and lysosomes. , Prussian blue staining was used to detect ferric ions inside cancer cells to evaluate the ion release of the Fe 3 O 4 phase from PA-SPIONs . It should be noted that the magnetite phase releases both Fe 2+ and Fe 3+ cations.…”

Section: Resultsmentioning

confidence: 99%

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Targeted Chemotherapy Based on Amplifying the Reactive Oxygen Species of Doxorubicin-Loaded Polyaspartamide-Encapsulated Iron Oxide Nanoparticles

Nguyễn

Kim

Ryu

et al. 2022

ACS Appl. Nano Mater.

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We report an innovative reactive oxygen species (ROS)-based chemotherapy mechanism using doxorubicin (Dox)-loaded polyaspartamide (PA)-encapsulated superparamagnetic iron oxide nanoparticles (SPIONs). PA encapsulates SPIONs (PA-SPIONs) to create biocompatible nanocarriers with an average diameter of 152 nm. PA is conjugated with Dox, biotin, and 3-amino-1,2,4-triazole (3AT) to perform different functions in chemotherapy. As a result, 3AT inhibits endogenous catalase, biotin improves cancer cell targeting and uptake, and Dox, in conjunction with iron ions, stimulates exogenous ROS production above the lethal threshold of cancer cells. The fluorescence signal results from Dox confirm that PA-SPIONs have a great affinity for 4T1 cancer cells. Because of the pH-sensitive cleavage function of the hydrazone bond, approximately 80% of Dox is released in the cancer cell environment (pH ∼ 5). In in vitro cytotoxicity, PA-SPIONs are highly cytotoxic to 4T1 cells, with less than 50% cell viability. The 2′,7′-dichlorofluorescein diacetate test demonstrates the ROS production of the nanocarriers, while the cell apoptosis assay confirms the cell death mechanism. In an in vivo experiment, PA-SPIONs inhibited the tumor growth rate, with a tumor volume 4.5 times lower than that of the control after 28 days. The individual roles of Dox and 3AT in tumor volume growth inhibition are also demonstrated. PA-SPIONs are demonstrated for biosafety and blood compatibility. Because the nanocarriers demonstrate the high efficacy of 4T1 cancer cell treatment, it likely opens the door for future advancements in cancer-targeted chemotherapy.

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Section: Resultsmentioning

confidence: 99%

“…16,17 Prussian blue staining was used to detect ferric ions inside cancer cells to evaluate the ion release of the Fe 3 O 4 phase from PA-SPIONs. 43 It should be noted that the magnetite phase releases both Fe 2+ and Fe 3+ cations. On the other hand, Prussian blue staining can only identify ferric ions.…”

Section: Resultsmentioning

confidence: 99%

Targeted Chemotherapy Based on Amplifying the Reactive Oxygen Species of Doxorubicin-Loaded Polyaspartamide-Encapsulated Iron Oxide Nanoparticles

Nguyễn

Kim

Ryu

et al. 2022

ACS Appl. Nano Mater.

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“…The cellular internalization of FeO NPs was observed by Prussian blue staining assay according to an earlier report [ 87 ], with some modifications. In brief, CS, CS/PVA, PD-FeO NPs (0.01%) and CS/PVA-PD-FeO NPs (0.01%) treated HEK-293 cells were incubated for 24 h at a 5% CO 2 incubator.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial and Wound Healing Properties of FeO Fabricated Chitosan/PVA Nanocomposite Sponge

et al. 2021

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Diabetic and anemia-associated diabetic wounds increase the considerable morbidity and mortality in people, as reported by clinical studies. However, no anemia-associated diabetic wound dressing materials have been developed until now. Hence, this study aimed to develop a nanocomposite scaffold composed of chitosan (CS), poly (vinyl alcohol) (PVA), and phytogenic iron oxide nanoparticles (FeO NPs), for accelerated anemia-associated diabetic wound healing. The aqueous leaves extract of Pinus densiflora (PD) was utilized for the synthesis of iron oxide nanoparticles (FeO NPs). TEM and elemental analysis confirmed smaller size PD-FeO NPs (<50 nm) synthesis with the combination of iron and oxide. In addition, in vitro biological studies displayed the moderate antioxidant, antidiabetic activities, and considerable antibacterial activity of PD-FeO NPs. Further, the different concentrations of PD-FeO NPs (0.01, 0.03, and 0.05%) incorporated CS/PVA nanocomposites sponges were developed by the freeze-drying method. The porous structured morphology and the presence of PD-FeO NPs were observed under FE-SEM. Among nanocomposite sponges, PD-FeO NPs (0.01%) incorporated CS/PVA sponges were further chosen for the in vitro wound-healing assay, based on the porous and water sorption nature. Furthermore, the in vitro wound-healing assay revealed that PD-FeO NPs (0.01%) incorporated CS/PVA has significantly increased the cell proliferation in HEK293 cells. In conclusion, the CS/PVA-PD-FeO NPs (0.01%) sponge would be recommended for diabetic wound dressing after a detailed in vivo evaluation.

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“…Для выявления в клетках ионов железа (III) образцы клеточных культур и гистологические срезы обрабатывали гексацианоферратом калия K4 [Fe(CN)6] по стандартной методике [4]. Клетки отмывали от среды, фиксировали 4% параформальдегидом в фосфатносолевом буфере (ФСБ) в течение 20 минут при комнатной температуре.…”

Section: верификация интернализации Ionp с помощью гексацианоферрата unclassified

Autologous mesenchymal stem cells in treatment of liver cirrhosis: evaluation of effectiveness and visualization method

Kotkas

Енукашвили

Asadulayev

et al. 2020

Science and Innovations in Medicine

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Through clinical observation, we present an assessment of the autologous mesenchymal stem cells effectiveness in treatment of liver cirrhosis of alimentary etiology. In order to determine the localization of the implanted cell structures, the stem cells were previously labeled with iron (II, III) oxide nanoparticles (IONPs). Further MRI visualization helped to detect the cell structures stained with iron oxide nanoparticles in the human body. In 6 months after the cell therapy, the patient underwent clinical and biochemical blood tests, MEGX test, elastography and subjective health assessment test. The tests data analysis revealed the improvement of the values of all examined parameters after the cell treatment. Also in 6 and 12 months after the treatment, a liver biopsy was performed from the area where the implanted stem cells were visualized. In histological examination of liver bioptates obtained from the area of MSC transplantation, the largest number of stained cells was observed in liver micronodes, as well as at the boundaries of micronodes and fibrous septa. A portion of the bioptate obtained in 12 months after transplantation was used to produce primary cell cultures. Before the first re-seeding of the cultures, cell colonies of both fibroblast-like morphology and epithelial were detected in them. Both types of colonies contained the particles. Conducting the cell therapy to a patient with liver cirrhosis of alimentary etiology contributed to improving the laboratory and instrumental examinations indicators. The patient had come through the treatment procedure satisfactorily, no complications were registered.

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D-mannose-Coating of Maghemite Nanoparticles Improved Labeling of Neural Stem Cells and Allowed Their Visualization by ex vivo MRI after Transplantation in the Mouse Brain

Cited by 20 publications

References 86 publications

Targeted Chemotherapy Based on Amplifying the Reactive Oxygen Species of Doxorubicin-Loaded Polyaspartamide-Encapsulated Iron Oxide Nanoparticles

Targeted Chemotherapy Based on Amplifying the Reactive Oxygen Species of Doxorubicin-Loaded Polyaspartamide-Encapsulated Iron Oxide Nanoparticles

Antimicrobial and Wound Healing Properties of FeO Fabricated Chitosan/PVA Nanocomposite Sponge

Autologous mesenchymal stem cells in treatment of liver cirrhosis: evaluation of effectiveness and visualization method

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