D-Ribose-Induced Glycation and Its Attenuation by the Aqueous Extract of Nigella sativa Seeds

Abstract

Background and Objectives: Glycation and oxidative stress are the major contributing factors responsible for diabetes and its secondary complications. Aminoguanidine, a hydrazine derivative, is the only approved drug that reduces glycation with its known side effects. As a result, research into medicinal plants with antioxidant and antiglycation properties is beneficial in treating diabetes and its consequences. This investigation aimed to examine the efficacy of the aqueous extract of Nigella sativa seeds against the D-ribose-induced glycation system. Materials and Methods: The suppression of α-amylase and α-glucosidase enzymes were used to assess the antidiabetic capacity. UV–Visible, fluorescence, and FTIR spectroscopy were used to characterize the Nigella sativa seed extract and its efficacy in preventing glycation. The inhibition of albumin glycation, fluorescent advanced glycation end products (AGEs) formation, thiol oxidation, and amyloid formation were used to evaluate the extracts’ antiglycation activity. In addition, the extent of glycoxidative DNA damage was analyzed using agarose gel electrophoresis. Results: The IC50 for the extract in the α-amylase and α-glucosidase enzyme inhibition assays were approximately 1.39 ± 0.016 and 1.01 ± 0.022 mg/mL, respectively. Throughout the investigation, it was found that the aqueous extract of Nigella sativa seeds (NSAE) inhibited the level of ketoamine, exerted a considerable drop in fluorescence intensity, and reduced carbonyl production and thiol modification when added to the D-ribose-induced glycation system. In addition, a reduction in the BSA-cross amyloid formation was seen in the Congo red, thioflavin T assay, and electrophoretic techniques. NSAE also exhibited a strong capability for DNA damage protection. Conclusion: It can be concluded that Nigella sativa could be used as a natural antidiabetic, antiglycation treatment and a cost-effective and environmentally friendly source of powerful bioactive chemicals.

Enzymatic Deglycation of Damaged Skin by Means of Combined Treatment of Fructosamine-3-Kinase and Fructosyl-Amino Acid Oxidase

“…Aminoguanidine (AG) is a hydrazine derivative and has been used as a representative compound for glycation inhibition and has served as a positive control in numerous experimental setups [20,21,[45][46][47]. AG hydrochloride (Sigma-Aldrich, St. Louis, MO, USA) was used as a positive deglycation agent.…”

“…AG hydrochloride (Sigma-Aldrich, St. Louis, MO, USA) was used as a positive deglycation agent. Given the known side effects shown in previous studies, AG is not used for clinical purposes [45,47].…”

Antiglycating Effects of Spirulina platensis Aqueous Extract on Glucose‐Induced Glycation of Bovine Serum Albumin

“…Simultaneously, parallel samples containing the respective extracts underwent an identical incubation protocol to rigorously evaluate and compare their efficacy in preventing glycation. Aminoguanidine (AG), a well‐established positive control, was consistently applied at a concentration of 1 mM [29,30] …”

“…This result aligns closely with the findings of Lai et al ( 2019), who reported a doubling time of 11.75 days for a similar culture. [29]…”

Unlocking the Therapeutic Potential of Stevia rebaudiana Bertoni: A Natural Antiglycating Agent and Non-Toxic Support for HDF Cell Health

“…A spectrum of both qualitative and quantitative methodologies has been developed, illuminating the intricacies of this phenomenon [7]. Traditional glycated materials undergo meticulous examination via diverse techniques, including spectrophotometric and electrophoretic methods [8]. Recent strides have introduced sophisticated tools like spectrofluorimetry, high-performance liquid chromatography (HPLC), and liquid chromatography-mass spectrometry (LC-MS), offering heightened precision in quantifying AGEs and evaluating glycation-related processes [9].…”