D-RNAi (Messenger RNA-antisense DNA Interference) as a Novel Defense System Against Cancer and Viral Infections.

Lin, Shi-Lung; Ying, Shao‐Yao

doi:10.2174/1568009013334151

Cited by 22 publications

(1 citation statement)

References 19 publications

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“… 27 – 29 siRNAs inhibit specific gene expression by inducing the sequence-specific degradation of homologous mRNA, which knocks down the gene product at the posttranscriptional level. 30 – 32 In the current study, wild-type (WT) and PXR-knockdown mice were used to assess the effects of PXR on the Tan IIA treatment of DSS-induced IBD. 14 Results indicated that Tan IIA could ameliorate DSS-induced IBD in a dose-dependent manner, and the effects were exerted by the activation of PXR and inhibition of the expression of inflammatory mediator genes in vivo.…”

Section: Introductionmentioning

confidence: 99%

Tanshinone IIA ameliorates dextran sulfate sodium-induced inflammatory bowel disease via the pregnane X receptor

Zhang¹,

Wang²,

Liang³

et al. 2015

DDDT

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Tanshinone IIA (Tan IIA) (C19H18O3) is one of the major active lipophilic components in a conventional Chinese medicine called danshen, and it has long been used in the People’s Republic of China and other neighboring countries to treat patients suffering from inflammatory bowel disease (IBD). Previous experiments by many teams determined which mechanism of Tan IIA is relevant to the treatment of IBD associated with inflammation and the pregnane X receptor (PXR). The current study demonstrated that Tan IIA is an efficacious PXR agonist and its ability to induce CYP3A4 mRNA and protein expression was mediated by the transactivation of PXR, a known target of abrogating inflammation in IBD. Clinical symptoms in mice and histological assessment data suggested that administration of Tan IIA in mice demonstrated significant protection and showed that in DSS-induced IBD it acts in a concentration-dependent manner. PXR-silenced mice treated with Tan IIA demonstrated low protection against DSS-induced mouse IBD and exacerbated the severity of IBD compared with wild-type mice; PXR-silenced mice demonstrated the necessity for PXR in Tan IIA-mediated upregulation of xenobiotic metabolism genes. The IBD treatment effects of Tan IIA are partially due to PXR-mediated upregulation of xenobiotic metabolism and downregulation of inflammatory mediators. The novel findings reported here may contribute to the effective utilization of Tan IIA and its derivatives as a PXR ligand in the treatment of human IBD. This suggests that Tan IIA may have considerable clinical utility.

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Section: Introductionmentioning

confidence: 99%

Tanshinone IIA ameliorates dextran sulfate sodium-induced inflammatory bowel disease via the pregnane X receptor

Zhang¹,

Wang²,

Liang³

et al. 2015

DDDT

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D-RNAi-Based Therapeutics

Lin

Ying

Apoptotic Pathways as Targets for Novel Therapies in Cancer and Other Diseases

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Recent Application of Intronic MicroRNA Agents in Cosmetics

Lin

Wu²,

Ying

2008

Current Perspectives in microRNAs (miRNA)

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Utilization of gene silencing effectors, such as microRNA (miRNA) and small hairpin RNA (shRNA), provides a powerful new strategy for human skin care in vivo, particularly for hyperpigmentation treatment and aging prevention. For example, tyrosinase (Tyr), a melanocytic membrane-bound glycoprotein, is the rate-limiting enzyme critical for melanin (black pigment) biosynthesis in skins and hairs. There are over 54 native microRNA capable of targeting human tyrosinase for skin whitening and lightening. In this study, we have designed a mir-434-5p homologue and used it to successfully demonstrate the feasibility of miRNA-mediated skin whitening in vitro and in vivo. Under the same experimental condition in trials, Pol-II-directed intronic mir-434-5p expression did not cause any detectable sign of cytotoxicity, whereas siRNAs targeting the same sequence induced certain non-specific mRNA degradation as previously reported. Because the intronic miRNA-mediated gene silencing pathway is tightly regulated by multiple intracellular surveillance systems, including Pol-II transcription, RNA splicing, exosome digestion and NMD processing, the current findings underscore the fact that intronic miRNA agents, such as mir-434-5p homologues, are effective, target-specific and safe to be used for skin whitening without any overt cytotoxic effect. Given that the human skins also express a variety of native miRNAs, we may re-design these miRNAs based on their individual functions for skin care, which will provide significant insights into areas of opportunity for new cosmetic interventions.Keywords microRNA (miRNA), intronic microRNA (Id-miRNA), mir-434-5p, tyrosinase (Tyr), hyaluronidase (Hyal), RNA interference (RNAi), skin whitening, antiaging, cosmetics.S.-Y. Ying (ed.) Current Perspectives in microRNAs (miRNA), 51

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D-RNAi (Messenger RNA-antisense DNA Interference) as a Novel Defense System Against Cancer and Viral Infections.

Cited by 22 publications

References 19 publications

Tanshinone IIA ameliorates dextran sulfate sodium-induced inflammatory bowel disease via the pregnane X receptor

Tanshinone IIA ameliorates dextran sulfate sodium-induced inflammatory bowel disease via the pregnane X receptor

D-RNAi-Based Therapeutics

Recent Application of Intronic MicroRNA Agents in Cosmetics

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