D₁Receptor in Interneurons of Macaque Prefrontal Cortex: Distribution and Subcellular Localization

Abstract

Working memory performance is influenced by dopamine activation of D1 family dopamine receptors in the prefrontal cortex; working memory performance is maximal at moderate stimulation of D1 family receptors and is reduced by either higher or lower levels of D1 stimulation. The neuronal mechanisms that underlie this complex relationship are not yet understood. Previous work from this laboratory has demonstrated that the D1 family receptors, D1and D5, are located in different compartments of pyramidal cells. Here we use an antibody specific to the D1receptor and double-label immunohistochemistry at the light and electron microscopic level to demonstrate that D1-like immunoreactivity (D1-LIR) is also present in interneurons. D1receptor is prevalent in parvalbumin-containing interneurons and is less common in calretinin-containing interneurons. At the ultrastructural level, D1-LIR is found associated with the Golgi apparatus and endoplasmic reticulum in the soma, with the membranes of vesicles in proximal dendrites, and with the plasma membrane on distal dendrites, where it is often located near asymmetric synapses. In addition, D1-LIR is also seen in presynaptic axon terminals, which give rise to symmetric synapses onto dendritic shafts and soma. These results raise the possibility that the circuit basis of working memory in the prefrontal cortex involves a D1-mediated inhibitory component.

Microdomains for Dopamine Volume Neurotransmission in Primate Prefrontal Cortex

“…The SSCs were almost exclusively localized in the perisomatic region of pyramidal cells, whereas they occurred rarely in perikarya and were virtually absent from the proximal processes of interneurons (see Materials and Methods for identification criteria). These rarely occurring SSCs in nonpyramidal neurons were never seen in association with DARs [see Khan et al (2001) for D 2 R, Muly et al (1998) for D 1 R]. In addition, both the D 5 R protein and D 5 R mRNA are localized predominantly in pyramidal neurons (Bergson et al, 1995a;Ariano et al, 1997;Ciliax et al, 2000).…”

神经细胞中多巴胺d1和d5受体不同亚细胞分布和胞内转运的特性

“…Potential mechanisms have been attributed to the different receptor subtypes, their differential affinities to agonist and their differential cellular or subcellular distributions [1,5,6,[31][32][33][34] .…”

D1-Like Dopamine Receptor Activation Modulates GABAergic Inhibition But Not Electrical Coupling between Neocortical Fast-Spiking Interneurons

“…Therefore, the use of dopamine or a nonspecific agonist in these experiments would activate both types of receptors, perhaps resulting in the physiological effects from activation of one receptor type canceling out those caused by agonism of the other (Trantham-Davidson et al, 2004). Parvalbumin-containing fast-spiking interneurons are particularly sensitive to D 1 -like receptor activation compared with calbindin-expressing, low-thresholdspiking cells, reflecting the preferential expression of this receptor in the former (Le Moine and Gaspar, 1998;Muly et al, 1998;Gorelova et al, 2002;Paspalas and Goldman-Rakic, 2005;Kroner et al, 2007). The proportion of PV-immunoreactive neurons that express the D 1 receptor is particularly high (Ͼ60%) in the infragranular layers.…”

“…We found that application of a D 1 -like agonist reduced IPSCs by approximately one-third. D 1 receptors are present on both presynaptic and postsynaptic compartments of FS cells (Muly et al, 1998;Paspalas and Goldman-Rakic, 2005), so it is conceivable that the D 1 -like receptor-mediated depression of IPSCs could have a presynaptic site of action, a postsynaptic mechanism, or a combination of both. Our finding that SKF81297 increased the coefficient of variation of IPSC amplitudes suggests that activation of presynaptic D 1 -like receptors affected the release of GABA and reduced the amplitude of GABAergic postsynaptic events.…”