D11, a novel monoclonal antibody specific for human mature macrophages and peripheral blood monocytes

Rudinskaya, T. D.; Полторанина, В. С.; Баранов, В. Н.; Petrovichev, Nicolas N.; Voytenkov, B. O.; Tretyakov, L.O.

doi:10.1016/0165-2478(92)90085-3

Cited by 3 publications

(1 citation statement)

References 16 publications

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“…These MoAbs belong to different epitope clusters [15]: D 10 to AB (N92), F 2 to F (N118) and F 5 to D (N113). The MoAb to macrophage antigen D 11 [16], an unrelated antibody, was introduced in the experiments as a negative control.…”

Section: Monoclonal Antibodiesmentioning

confidence: 99%

Conformational Variants of Human Alpha-Fetoprotein

Karamova

Yazova²,

Goussev³

et al. 1998

Tumor Biol

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The immunological heterogeneity of human α-fetoprotein (AFP) was demonstrated using immunoaffinity electrochromatography on monoclonal antibodies (MoAbs) to 3 non-cross-reacting epitopes of this protein. At least 4 subfractions expressing different epitopes were found in the native AFP. These subfractions demonstrated molecular weights similar to the major component of the original AFP. The difference between epitope F₅-positive and F₅-negative subfractions disappeared when epitope-negative subfraction was conformationally changed after fixation onto nitrocellulose membrane (NCM). Thus, the epitope under study exists in two forms on the native human AFP molecule: an open and a cryptic form. The cryptic form could be revealed after partial denaturation by fixation on NCM. The epitope variants of AFP could possess different functions in multifunctional AFP. The AFP epitope heterogeneity found in this work should be taken into account when constructing diagnostic AFP kits and when isolating purified AFP using anti-AFP MoAbs.

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Section: Monoclonal Antibodiesmentioning

confidence: 99%

Conformational Variants of Human Alpha-Fetoprotein

Karamova

Yazova²,

Goussev³

et al. 1998

Tumor Biol

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Reactivity of anti-macrophage monoclonal antibody D11 in human leukemia and malignant lymphoma

et al. 1996

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We have studied the reactivity patterns of a previously described pan-macrophage monoclonal antibody (MAb) D11 in 324 cases of acute leukemia and malignant lymphoma (ML). Reaction of D11 in tissue sections was restricted to histiocytes and macrophages. In non-Hodgkin's ML, D11 helped to confirm or to establish the histiocytic nature in 8 of 96 cases, i.e., in 4 of 6 histiocytic MLs; 2 of 13 anaplastic large-cell lymphomas; 1 of 4 large-cell immunoblastic clear-cell MLs; and 1 of 2 histiocytosis X cases. Positive reaction of D11 in acute lymphoblastic leukaemia (ALL) was found in 9 of 86 cases (all belonging to early B-lineage leukemia), of which 4 were CD34-positive and 5 co-expressed 1 or more myeloid/monocytic antigens. MAb D11 did not react in 42 cases of acute-myeloblastic-leukemia (AML) FAB variants M0-M5, except 1 acute mixed-lineage leukemia M1/pre-pre-B. Comparative study of the MAb D11 and a standard CD68 MAb KP- 1 showed that the antigens belong to different epitopes of different molecules.

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Characterization of an enzyme-linked immunosorbent assay for soluble CD163

Møller

Hald²,

Moestrup³

2002

Scandinavian Journal of Clinical and Laboratory Investigation

151

126

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We have recently identified a soluble plasma form of CD163 sCD163, the macrophage receptor for clearance of haptoglobin-haemoglobin complexes, and we have observed highly elevated levels of sCD163 in subgroups of haematological patients. In the present study, we describe the optimization and characterization of a sandwich ELISA for the determination of the concentration of sCD163 in plasma and serum. The optimal concentrations of antibodies were determined systematically and the assay was calibrated by CD163 purified from human spleen membranes. The minimum detection limit was below 6.25 microg/L. Recovery of CD163 added to plasma samples was 106%. The concentration of sCD163 in paired serum and plasma samples correlated well (r2=0.99); however, serum levels were 1.1 times higher than the plasma levels. The addition of haptoglobin-haemoglobin complexes did not influence the assay. A very high stability of sCD163 was measured in whole blood and in plasma subjected to different temperatures and after repeated freezing and thawing.

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D11, a novel monoclonal antibody specific for human mature macrophages and peripheral blood monocytes

Cited by 3 publications

References 16 publications

Conformational Variants of Human Alpha-Fetoprotein

Conformational Variants of Human Alpha-Fetoprotein

Reactivity of anti-macrophage monoclonal antibody D11 in human leukemia and malignant lymphoma

Characterization of an enzyme-linked immunosorbent assay for soluble CD163

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