D1S80 Population Data in African Americans, Caucasians, Southeastern Hispanics, Southwestern Hispanics, and Orientals

Abstract

Allele frequencies for the locus D1S80 were determined in African American, Caucasian, Southeastern Hispanic, Southwestern Hispanic, and Oriental sample populations using the polymerase chain reaction and subsequent electrophoresis and silver staining of the amplified products. Due to the presence of anodal and cathodal electrophoretic variants (in reference to the steps in an allelic ladder), allele frequencies were established using a classification protocol based on the steps in the allelic ladder. All sample populations met Hardy-Weinberg expectations for D1S80. In addition, there was no evidence for association of alleles between the loci D1S80 and D1S7. The product of allele frequencies from the data from the sample populations in this study can be used in forensic analyses and paternity tests to estimate the frequency of a D1S80 DNA genotype.

DNA Profile Similarity in a Subdivided Population

“…assign a similarity value S to each pair, and calculate the average similarity for all ran dom pairs. The answer obtained is identical to equation (9). An algebraic enumeration of all pairs is tedious.…”

“…Em pirical databases of DNA profile frequencies have only recently come into being [9], and considerable data are being accumulated [10], Since the VNTR and other markers being used for forensic studies are highly polymor phic, a very large number of genotypes, each individually rare, are evident. As discussed by Chakraborty [11], this property makes accu rate direct estimation of DNA profile fre quencies difficult.…”

Genetic characterization of the population of São Luís, MA, Brazil

“…#-the vWA1*13 and D1S80*17 less frequent alleles have been collapsed for this analysis. [Alonso et al (1993);Bell et al (2000); Boerwinkle et al (1989); Budowle et al (1995); Buresi et al (1995); Chakraborty et al (1991);D'Aloja et al (1992); Deka et al (1992;1994); Destro-Bisol et al (1994;; Evans et al (1993); Falcone et al (1995); Flores et al (2001); Gamero et al (2000;; Gené et al (1993;; Gusmão et al (1997;; Gutowski et al (1995); Halos et al (1999);Hammond et al (1994); Heidrich et al (1995); Hixon et al (1993);Latorra et al (1994); Maviglia et al (2001); …”

Minisatellite DNA Markers in Population Studies

“…The spectrum and frequencies of D1S80 alleles have been described fairly comprehensively, as this locus has been used intensively in criminalistics and forensic medical examinations (Kasai et al, 1990). Subsequently, Budowle et al suggested the possibility of using D1S80 to differentiate populations (Budowle et al, 1991(Budowle et al, , 1995. The first investigation of the variability of this locus on a global scale, namely, in 43 populations from different regions of the world, was published by Duncan et al in 1996(Duncan et al, 1996.…”

“…The first major allele, which contains 18 repeat units, occurs in 5.5-9% of sub-Saharan African populations, in 15-21% of Asian populations, and in 13-35% of European populations. The second major allele (24 repeats) has a frequency of 26-45% in Europeans, 6-29% in sub-Saharan Africans, and 17-24% in Asians (Das & Mastana, 2003;Duncan et al, 1996-97;Budowle et al, 1991Budowle et al, , 1995Herrera et al, 2004;Sajantila et al, 1992).…”