D2 dopamine receptor localization on striatonigral neurons

Ariano, Marjorie A.; Stromski, C.; Smyk-Randall, Elzbieta M.; Sibley, David R.

doi:10.1016/0304-3940(92)90753-t

Cited by 61 publications

(36 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This model has been supported by the findings of numerous investigators using varied approaches. However, more recent reports have suggested that a significant amount of D1 and D2 receptor colocalization may occur on striatal projection neurons (3)(4)(5)(6)(7)(8). This issue is an important one because if coexpression of the two receptors on striatal efferents is common, this would imply that dopamine does not separately and differentially regulate striatonigral and striatopallidal output in the manner predicted by the basal ganglia model.…”

mentioning

confidence: 91%

“…The two efferent populations have been distinguished either by retrograde labeling or by their expression of projection-related peptide markers (mRNAs for substance P or dynorphin for striatonigral neurons and enkephalin for striatopallidal neurons). These studies yielded vastly different estimates of the extent of receptor colocalization ranging from virtually none (2,10,12,13) to as much as 50-60% of striatal neurons expressing both receptors (3,6,7,11). Part of the discrepancy in these estimates may be a result of the methods for distinguishing the two neuronal populations.…”

mentioning

confidence: 93%

“…A majority of the previous studies have measured the cellular expression or distribution of D1 and D2 receptor mRNAs (2-5, 9-11), or the binding of selective ligands (8) or antibodies to the receptors (6,12,13). The two efferent populations have been distinguished either by retrograde labeling or by their expression of projection-related peptide markers (mRNAs for substance P or dynorphin for striatonigral neurons and enkephalin for striatopallidal neurons).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Expression of a dopamine D2 receptor-activated K ⁺ channel on identified striatopallidal and striatonigral neurons

Waszczak

Martin²,

Greif³

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

One view of the efferent circuitry of the basal ganglia holds that dopamine D1 and D2 receptors are segregated to striatonigral and striatopallidal neurons, respectively. The present studies investigated whether functional D2-like receptors are, in fact, restricted to striatopallidal neurons. Single, freshly dissociated cells from rat striatum were identified as either striatonigral or striatopallidal projection neurons by f luorescence retrograde labeling. By using cell-attached patch-clamp recordings, neurons of each efferent group were evaluated for the presence of a D2-like receptor-activated 85-pS K ؉ channel as a measure of receptor function. We now report the presence of this D2 receptormediated response on both striatal efferent populations, but we observed an approximately 2-fold higher likelihood of encountering the channel on pallidal-versus nigral-projecting neurons. The channel's conductance properties appeared identical in both groups of neurons, but there was a significantly greater open probability for channels detected on striatopallidal neurons. These results indicate that functional D2 receptors are not segregated to striatopallidal neurons, but may be expressed in a higher proportion of, or at a higher density and͞or efficiency of coupling on, pallidal-versus nigral-projecting striatal efferents.Medium spiny neurons of the corpus striatum project to the substantia nigra and to the globus pallidus. The current functional model of the basal ganglia hypothesizes that dopamine exerts opposite physiological effects on striatonigral and striatopallidal efferent populations (excitatory and inhibitory, respectively) (1), and that these opposing effects of dopamine are mediated by a segregated and specific expression of D1 and D2 dopamine receptors, respectively, on the two efferent cell groups (2). This model has been supported by the findings of numerous investigators using varied approaches. However, more recent reports have suggested that a significant amount of D1 and D2 receptor colocalization may occur on striatal projection neurons (3-8). This issue is an important one because if coexpression of the two receptors on striatal efferents is common, this would imply that dopamine does not separately and differentially regulate striatonigral and striatopallidal output in the manner predicted by the basal ganglia model. The model would then need to be revised to accommodate the possibility that the two receptors interact on at least a subpopulation of striatal neurons to regulate the activities of both efferent cell groups.The controversy surrounding the degree of D1 and D2 receptor colocalization stems in part from limitations of the previously used techniques for both detecting the receptors and identifying the two projection populations. A majority of the previous studies have measured the cellular expression or distribution of D1 and D2 receptor mRNAs (2-5, 9-11), or the binding of selective ligands (8) or antibodies to the receptors (6, 12, 13). The two efferent populations have been di...

show abstract

mentioning

confidence: 91%

mentioning

confidence: 93%

mentioning

confidence: 99%

See 1 more Smart Citation

Expression of a dopamine D2 receptor-activated K ⁺ channel on identified striatopallidal and striatonigral neurons

Waszczak

Martin²,

Greif³

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Surmeier et al (1992Surmeier et al ( , 1993Surmeier et al ( , 1996 showed that both efferent groups respond electrophysiologically to both D 1 and D 2 receptor agonists, and many contain mRNA for both classes of receptor. MeadorWoodruff et al (1991) and Lester et al (1993) found that D 1 and D 2 mRNAs were coexpressed by 27 to 33% of striatal efferents, whereas Ariano et al (1992) observed D 2 receptor immunoreactivity on a minimum of 60% of striatonigral neurons. More strikingly, Aizman et al (2000) showed complete colocalization of the two receptors on neurons from embryonic rat striatum.…”

Section: Downloaded Frommentioning

confidence: 99%

Effects of Individual and Concurrent Stimulation of Striatal D₁ and D₂ Dopamine Receptors on Electrophysiological and Behavioral Output from Rat Basal Ganglia

Waszczak¹,

Martin²,

Finlay³

et al. 2002

J Pharmacol Exp Ther

View full text Add to dashboard Cite

Bilateral infusions of d-amphetamine into the rat ventral-lateral striatum (VLS) were previously shown to cause a robust behavioral activation that was correlated temporally with a net increase in firing of substantia nigra pars reticulata (SNpr) neurons, a response opposite predictions of the basal ganglia model. The current studies assessed the individual and cooperative contributions of striatal D 1 and D 2 dopamine receptors to these responses. Bilateral infusions into VLS of the D 1 /D 2 agonist apomorphine (10 g/l/side) caused intense oral movements and sniffing, and an overall increase in SNpr cell firing to 133% of basal rates, similar to effects of d-amphetamine. However, when striatal D 2 receptors were stimulated selectively by infusions of quinpirole (30 g/l/side) ϩ the D 1 antagonist R-(ϩ) -7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine (SCH 23390; 10 g/l/ side), no behavioral response and only modest and variable changes in SNpr cell firing were observed. Selective stimulation of striatal D 1 receptors by (Ϯ) 6-chloro-APB hydrobromide (SKF 82958; 10 g/l/side) ϩ the D 2 antagonist cis-N-(1-benzyl-2-methyl-pyrrolidin-3-yl)-5-chloro-2-methoxy-4-methyl-aminobenzamide (YM 09151-2; 2 g/l/side) caused a weak but sustained increase in oral movements and modestly increased SNpr cell firing, but neither response was of the magnitude observed with apomorphine. When the two agonists were infused concurrently, however, robust oral movements and sniffing again occurred over the same time period that a majority of SNpr cells exhibited marked, sometimes extreme and fluctuating, changes in firing (net increase, 117% of basal rates). These data confirm that concurrent striatal D 1 /D 2 receptor stimulation elicits a strong motor activation that is correlated temporally with a net excitation rather than inhibition of SNpr firing, and reveal that D 1 and D 2 receptors interact synergistically within the striatum to stimulate both forms of output.The basal ganglia functional model predicts that dopamine, by stimulating the striatonigral pathway via D 1 receptors and inhibiting the striatopallidal pathway via D 2 receptors, should inhibit output from the substantia nigra pars reticulata and internal pallidal segment (SNpr/GPi). Inhibition of output from the SNr/GPi should in turn disinhibit the thalamus to facilitate movement (for review, see Alexander and Crutcher, 1990). Many of the predictions of the model have been supported by electrophysiological studies in humans with Parkinson's disease and animal models of this disorder (Filion and Tremblay, 1991;Bergman et al., 1994;Papa et al., 1999;Levy et al., 2001). However, the question of whether these predictions are valid and supported in normal animals without dopaminergic lesions has been less rigorously addressed.A definitive test of this hypothesis has been made difficult by the complexity of the circuitry, in particular by the fact that D 1 and D 2 receptors are expressed not only within the striatum but also in other nuclei of th...

show abstract

“…comm.). Cellular binding patterns for dopamine receptors are different for different neurons also in striatal slices (132), (133), (134). New techniques of laser capture microscopy may be able to give clear answers on the question of cell-specificity (M. Ariano, pers.comm).…”

Section: Synapse-and Cell-specific Receptor Regulationmentioning

confidence: 99%

Regulation of neuromodulator receptor efficacy—implications for whole-neuron and synaptic plasticity

Scheler

2004

Progress in Neurobiology

View full text Add to dashboard Cite

Membrane receptors for neuromodulators (NM) are highly regulated in their distribution and efficacy -a phenomenon which influences the individual cell's response to central signals of NM release. Even though NM receptor regulation is implicated in the pharmacological action of many drugs, and is also known to be influenced by various environmental factors, its functional consequences and modes of action are not well understood. In this paper we summarize relevant experimental evidence on NM receptor regulation (specifically dopamine D1 and D2 receptors) in order to explore its significance for neural and synaptic plasticity. We identify the relevant components of NM receptor regulation (receptor phosphorylation, receptor trafficking and sensitization of second-messenger pathways) gained from studies on cultured cells. Key principles in the regulation and control of short-term plasticity (sensitization) are identified, and a model is presented which employs direct and indirect feedback regulation of receptor efficacy. We also discuss long-term plasticity which involves shifts in receptor sensitivity and loss of responsivity to NM signals. Finally, we discuss the implications of NM receptor regulation for models of brain plasticity and memorization. We emphasize that a realistic model of brain plasticity will have to go beyond Hebbian models of long-term potentiation and depression. Plasticity in the distribution and efficacy of NM receptors may provide another important source of functional plasticity with implications for learning and memory.

show abstract

D2 dopamine receptor localization on striatonigral neurons

Cited by 61 publications

References 21 publications

Expression of a dopamine D2 receptor-activated K ⁺ channel on identified striatopallidal and striatonigral neurons

Expression of a dopamine D2 receptor-activated K ⁺ channel on identified striatopallidal and striatonigral neurons

Effects of Individual and Concurrent Stimulation of Striatal D₁ and D₂ Dopamine Receptors on Electrophysiological and Behavioral Output from Rat Basal Ganglia

Regulation of neuromodulator receptor efficacy—implications for whole-neuron and synaptic plasticity

Contact Info

Product

Resources

About

D2 dopamine receptor localization on striatonigral neurons

Cited by 61 publications

References 21 publications

Expression of a dopamine D2 receptor-activated K + channel on identified striatopallidal and striatonigral neurons

Expression of a dopamine D2 receptor-activated K + channel on identified striatopallidal and striatonigral neurons

Effects of Individual and Concurrent Stimulation of Striatal D1 and D2 Dopamine Receptors on Electrophysiological and Behavioral Output from Rat Basal Ganglia

Regulation of neuromodulator receptor efficacy—implications for whole-neuron and synaptic plasticity

Contact Info

Product

Resources

About

Expression of a dopamine D2 receptor-activated K ⁺ channel on identified striatopallidal and striatonigral neurons

Expression of a dopamine D2 receptor-activated K ⁺ channel on identified striatopallidal and striatonigral neurons

Effects of Individual and Concurrent Stimulation of Striatal D₁ and D₂ Dopamine Receptors on Electrophysiological and Behavioral Output from Rat Basal Ganglia