Dabsyl derivatisation as an alternative for dansylation in the detection of biogenic amines in fermented meat products by reversed phase high performance liquid chromatography

Mey, Eveline De; Drabik-Markiewicz, Gabriela; Maere, Hannelore De; Peeters, M.‐C.; Derdelinckx, Guy; Paelinck, Hubert; Kowalska, Teresa

doi:10.1016/j.foodchem.2011.07.124

Cited by 70 publications

(30 citation statements)

References 19 publications

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“…It is obvious that the obtained limits of detection (LODs) were quite satisfying. Also, there are some HPLC studies presented, employing benzoyl chloride [45], 2-chloro-1,3-dinitro-5-(trifluoromethyl)-benzene (CNBF) [46,47], diethyl ethoxymethylenemalonate (DEEMM) [48], 9-fluorenylmethyl chloroformate (FMOC) [31], 1-naphthylisothiocyanate (NITC) [49], phenyl isothiocyanate (PITC) [50] or dabsyl chloride (Dbs-Cl) [51] with UV detection, obtaining low LODs, too.…”

Section: Chromatographicmentioning

confidence: 99%

Emerging Trends in Biogenic Amines Analysis

Neofotistos¹,

Tsagkaris²,

Danezis³

et al. 2019

Biogenic Amines

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Biogenic amines are low-molecular-mass substances, essential for proper health for all organisms. These compounds could be detrimental to human health with various toxicological effects when they are present in high concentrations. Therefore, biogenic amines monitoring in food samples is a matter of utmost importance, and their accurate determination is considered indispensable. Under this context, we provide an overview over the most widely employed analytical techniques for biogenic amines determination such as chromatographic techniques and biosensors, emphasizing on new approaches. A critical comparison of the techniques is also given, presenting their advantages and drawbacks regarding important analytical characteristics such as sensitivity. Finally, we focus on foods in which biogenic amines mainly occur such as fish, meat and wine and other fermented products.

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Section: Chromatographicmentioning

confidence: 99%

Emerging Trends in Biogenic Amines Analysis

Neofotistos¹,

Tsagkaris²,

Danezis³

et al. 2019

Biogenic Amines

View full text Add to dashboard Cite

show abstract

“…Furthermore, most of the conventional analytical methods need some tedious pretreatment steps, for example, preextraction, clean-up, and nitrogen drying for concentration before determination. Moreover, the classic extraction methods, such as LLE [22,23], SPE [24][25][26], can cause environmental pollution and harmful to manipulator owing to the consumption of a large amount of organic solvents (at the multiple milliliter level). To overcome these shortcoming, new sample preparation microextraction techniques, such as SPME [27,28], have gradually replaced SPE as an environmentally friendly pretreatment method because it possesses rapid, simple, sensitive characteristics, needs no or little organic extraction reagent, easy manipulation, linkup with detection instruments, and direct injection without elution while coupling with GC-MS for detection [29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Environmentally friendly solid‐phase microextraction coupled with gas chromatography and mass spectrometry for the determination of biogenic amines in fish samples

Huang

Gan

et al. 2016

J of Separation Science

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In this work, a facile and environmentally friendly solid-phase microextraction assay based on on-fiber derivatization coupled with gas chromatography and mass spectrometry was developed for determining four nonvolatile index biogenic amines (putrescine, cadaverine, histamine, and tyramine) in fish samples. In the assay, the fiber was firstly dipped into a solution with isobutyl chloroformate as derivatization reagent and isooctane as extraction solvent. Thus, a thin organic liquid membrane coating was developed. Then the modified fiber was immersed into sample solution to extract four important bioamines. Afterwards, the fiber was directly inserted into gas chromatography injection port for thermal desorption. 1,7-Diaminoheptane was employed as internal standard reagent for quantification of the targets. The limits of detection of the method were 2.98-45.3 μg/kg. The proposed method was successfully applied to the detection of bioamines in several fish samples with recoveries ranging 78.9-110%. The organic reagent used for extraction was as few as microliter that can greatly reduce the harm to manipulator and environment. Moreover, the extraction procedures were very simple without concentration and elution procedures, which can greatly simplify the pretreatment process. The assay can be extended to the in situ screening of other pollutant in food safety by changing the derivatization reagent.

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“…HPLC methods use various types of detectors, such as UV–vis (De Mey et al . , ; Kang, Xiao, Huang, & Gu, ; Palego et al . , ), fluorescence (Frank & Powers, ; Jaworska et al .…”

Section: Introductionmentioning

confidence: 99%

An HPLC method for the determination of selected amino acids in human embryo culture medium

Drábková

Andrlová

Kanďár

2016

Biomedical Chromatography

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A method for the determination of selected amino acids in culture medium using HPLC with fluorescence detection is described. Twenty hours after intra-cytoplasmic sperm injection, one randomly selected zygote was transferred to the culture medium. After incubation (72 h after fertilization), the culture medium in which the embryo was incubated and blank medium was immediately stored at -80°C. Filtered medium samples were derivatized with ortho-phthalaldehyde (naphthalene-2,3-dicarboxaldehyde), forming highly fluorescent amino acids derivatives. Reverse-phase columns (LichroCART, Purospher STAR RP or Ascentis Express C ) were used for the separation. The derivatives were analyzed by gradient elution with a mobile phase containing ethanol and sodium dihydrogen phosphate. The analytical performance of this method is satisfactory for all amino acids; the intra-assay coefficients of variation were <10% and quantitative recoveries were between 95.5 and 104.4%. Changes in the levels of selected amino acids before and after human embryo cultivation were observed. After embryo incubation, the levels of all amino acids in the medium were increased, apart from aspartate and asparagine. After the cultivation of some embryos, amino acids which were not part of the medium were detected. Low amino acids turnover was observed in some embryos.

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Dabsyl derivatisation as an alternative for dansylation in the detection of biogenic amines in fermented meat products by reversed phase high performance liquid chromatography

Cited by 70 publications

References 19 publications

Emerging Trends in Biogenic Amines Analysis

Emerging Trends in Biogenic Amines Analysis

Environmentally friendly solid‐phase microextraction coupled with gas chromatography and mass spectrometry for the determination of biogenic amines in fish samples

An HPLC method for the determination of selected amino acids in human embryo culture medium

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