Dahlia variabilis cultivar ‘Seattle’ as a model plant for anthochlor biosynthesis

Walliser, Benjamin; Lucaciu, Calin Rares; Molitor, Christian; Marinovic, Silvija; Nitarska, Daria; Aktaş, Didem; Rattei, Thomas; Kampatsikas, Ioannis; Stich, Karl; Haselmair-Gosch, Christian; Halbwirth, Heidi

doi:10.1016/j.plaphy.2020.12.016

Cited by 11 publications

(9 citation statements)

References 42 publications

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“…S2), indicating these genes are not involved in isoliquiritigenin biosynthesis in dahlia. Similar result was reported by Walliser et al (2021) that expression level of CHR-like genes (CHRl1-CHRl3) could not explain the butein biosynthesis.…”

Section: Discussionsupporting

confidence: 87%

Identification of a novel chalcone reductase gene for isoliquiritigenin biosynthesis in dahlia (Dahlia variabilis)

Ohno

Yamada

Maruyama

et al. 2022

Preprint

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Butein is one of flavonoids conferring bright yellow flower color and is a precursor of aurone in some species. Butein is synthesized by two steps, 3-malonyl CoA and 4-coumaloyl CoA are converted to isoliquiritigenin in the first step, and then isoliquiritigenin is converted to butein in the second step. In the first step, chalcone synthase (CHS) and chalcone reductase (CHR) catalyze this reaction, however, CHR has been reported for the isoflavone biosynthesis pathway in legumes, and CHR for butein biosynthesis has not yet been isolated. In this study, we report CHR that is evolutionally different gene from legume species is involved in isoliquiritigenin biosynthesis in dahlia. To isolate CHR gene, we conducted comparative RNA-seq analysis between ‛Shukuhai' and its butein-loss lateral mutant ‛Rinka'. We found DvCHR showed significant difference in expression levels that encodes an aldo-keto reductase (AKR) 13 family protein, which was phylogenetically different from legume CHRs belonging to AKR4A family. Gene expression levels and genotype of DvCHR were correlated with butein accumulation among various dahlia cultivars. Though single over expression of DvCHR was not able to accumulate isoliquiritigenin in tobacco, co-overexpression of DvCHR with a chalcone glucosyltransferase Am4'CGT and a MYB transcription factor CaMYBA successfully induced isoliquiritigenin accumulation. In addition, DvCHR homologous gene expression was detected from butein or aurone accumulating Coreopsideae species but not from non-butein or non-aurone accumulating Asteraceae species. These results indicated DvCHR functions as chalcone reductase for butein biosynthesis in dahlia, and isoliquiritigenin biosynthesis in Coreopsideae species has been developed independently from legume species.

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Section: Discussionsupporting

confidence: 87%

Identification of a novel chalcone reductase gene for isoliquiritigenin biosynthesis in dahlia (Dahlia variabilis)

Ohno

Yamada

Maruyama

et al. 2022

Preprint

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“…Due to the presence exclusively in the veins ( Supplementary Figure S3 ), the outer part of these varieties appear cream. The absence of anthochlors in the pure white and the faint purple genotypes points at the absence of CHR activity, which cannot be proven, as the formation of 6′-deoxychalcones in the Asteraceae species is not sufficiently understood so far [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…Isoliquiritigenin, butein, apigenin, luteolin, naringenin, eriodictyol, dihydrokaempferol, dihydroquercetin, marein, maritimein, peonidin and cyanidin were purchased from Extrasynthese (Genay, France). Okanin and maritimetin were obtained from marein and maritimein by enzymatic hydrolysis, as described previously [ 32 ]. Lutein (≥95%) and β-carotene (≥98%) were obtained from Extrasynthese (Genace, France).…”

Section: Methodsmentioning

confidence: 99%

The (Bio)chemical Base of Flower Colour in Bidens ferulifolia

Walliser

Marinovic

Kornpointner

et al. 2022

Plants

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Bidens ferulifolia is a yellow flowering plant, originating from Mexico, which is increasingly popular as an ornamental plant. In the past few years, new colour combinations ranging from pure yellow over yellow-red, white-red, pure white and purple have emerged on the market. We analysed 16 Bidens ferulifolia genotypes to provide insight into the (bio)chemical base underlying the colour formation, which involves flavonoids, anthochlors and carotenoids. In all but purple and white genotypes, anthochlors were the prevalent pigments, primarily derivatives of okanin, a 6′-deoxychalcone carrying an unusual 2′3′4′-hydroxylation pattern in ring A. The presence of a cytochrome-P450-dependent monooxygenase introducing the additional hydroxyl group in position 3′ of both isoliquiritigenin and butein was demonstrated for the first time. All genotypes accumulate considerable amounts of the flavone luteolin. Red and purple genotypes additionally accumulate cyanidin-type anthocyanins. Acyanic genotypes lack flavanone 3-hydroxylase and/or dihydroflavonol 4-reductase activity, which creates a bottleneck in the anthocyanin pathway. The carotenoid spectrum was analysed in two Bidens genotypes and showed strong variation between the two cultivars. In comparison to anthochlors, carotenoids were present in much lower concentrations. Carotenoid monoesters, as well as diesters, were determined for the first time in B. ferulifolia flower extracts.

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“…Only geranium and cyanidin are contained in gerbera (Elomaa et al 2003). There are 20,000 cultivars of dahlia, and its ower color is mainly determined by Xanthein, anthocyanins and other avonoids (Walliser et al 2021). In Asteraceae vegetablesLactuca sativa and Gynura bicolor DC, anthocyaninsimprove the quality of vegetables as a nutrient (Shimizu et al 2011, Su et al 2020, Šuštar-Vozlič et al…”

Section: Introductionmentioning

confidence: 99%

Analysis and Verification of the Conserved Myb Binding Element in the Dfr Promoter in Compositae

guo

Shi

et al. 2022

SSRN Journal

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Dahlia variabilis cultivar ‘Seattle’ as a model plant for anthochlor biosynthesis

Cited by 11 publications

References 42 publications

Identification of a novel chalcone reductase gene for isoliquiritigenin biosynthesis in dahlia (Dahlia variabilis)

Identification of a novel chalcone reductase gene for isoliquiritigenin biosynthesis in dahlia (Dahlia variabilis)

The (Bio)chemical Base of Flower Colour in Bidens ferulifolia

Analysis and Verification of the Conserved Myb Binding Element in the Dfr Promoter in Compositae

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