Daidzein enhances intramuscular fat deposition and improves meat quality in finishing steers

Zhao, Xianghui; Zhou, Yang; Bao, Lin-Bin; Wang, Can-Yu; Zhou, Shan; Gong, Jianming; Fu, Chuan-Bian; Xu, Long; Chan-juan, Liu; Qu, Mingren

doi:10.1177/1535370214564755

Cited by 24 publications

(23 citation statements)

References 36 publications

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“…There was a greater concentration of daidzein in SH than in SHS, indicating that the saponins might increase the microbial utilization of daidzein, which would be beneficial to animals. Previous results suggested that supplemental daidzein can affect lipid metabolism by enhancing intramuscular fat deposition and improves meat quality in finishing steers (Zhao et al, 2015). Squalene is an intermediate metabolite in the synthesis of cholesterol.…”

Section: Discussionmentioning

confidence: 99%

Saponin-Induced Shifts in the Rumen Microbiome and Metabolome of Young Cattle

et al. 2019

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The aim of this study was to explore the effects of saponins on the rumen microbiota and the ruminal metabolome. Alfalfa hay (AH) and soybean hulls (SH) were used as fiber sources for the control diets. The AH and SH diets were supplemented with tea saponins resulting in two additional diets named AHS and SHS, respectively. These 4 diets were fed to 24 young male Holstein cattle ( n = 6 per diet). After 28 days of feeding, the rumen fluid from these cattle was collected using an oral stomach tube. Illumina MiSeq sequencing and ultrahigh-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) were used to investigate the changes in the ruminal microbes and their metabolites. The relative abundance of Prevotellaceae_YAB2003 increased, while Ruminococcaceae_NK4A214 and Lachnospiraceae_NK3A20 decreased in SHS and AHS compared to SH and AHS, respectively. Feeding SHS resulted in higher ruminal concentrations of squalene, lanosterol, 3-phenylpropanoic acid, and citrulline compared to SH. The different microbial genes predicted by Tax4Fun were involved in amino sugar and nucleotide sugar metabolism. The pathways of arginine and proline metabolism, purine metabolism, and pyrimidine metabolism were enriched by different metabolites. Moreover, in the SH group, a positive correlation was observed between Prevotella_1 ( Bacteroidetes ), Prevotellaceae_YAB2003 ( Bacteroidetes ), and Christensenellaceae_R.7 ( Firmicutes ), and the metabolites, including citrulline, lanosterol, and squalene. The increased abundances of Prevotella_1 , Ruminococcaceae_UCG.002 , and Prevotellaceae_YAB2003 might result in increased fiber digestion and nutrient utilization but nutrient digestion was not measured in the current study. In summary, saponins have the ability to modulate the ruminal microbial community and ruminal metabolites and thus affect the rumen environment. However, the response seems to be dependent on the composition of the basal diet. This study provides a comprehensive overview of the microbial and biochemical changes in the rumen of cattle fed saponins.

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Section: Discussionmentioning

confidence: 99%

Saponin-Induced Shifts in the Rumen Microbiome and Metabolome of Young Cattle

et al. 2019

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“…Thesoy isoflavone, daidzein, is used as a phytoestrogen for treating cardiovascular disease [10], osteoporosis [11] and lower cholesterol [12]. Moreover, daidzein has been reported to inhibit the proliferation of human breast cancer MDA-MB-453 cells [13]and cervical cancer Hela cells in vitro [14].…”

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confidence: 99%

Daidzein exerts anti-tumor activity against bladder cancer cells via inhibition of FGFR3 pathway

He¹,

Wu²,

Cao³

et al. 2016

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Bladder cancer is one of the causes of cancer‑related death and has a high mortality rate. Daidzein, a natural isoflavone compound predominantly extracted from soybeans, has been reported to exhibit several bioactivities, including anti-tumor. However, the effects of daidzein on bladder cancer remains unrevealed. Here we investigated the effects and molecular mechanisms of daidzein on bladder cancer using multiple in vitro cell lines and in vivo xenograft mice studies. Our results showed that daidzein reduced cell viability in a time- and concentration‑dependent manner. Daidzein significantly impaired colony formation, caused G1/S cell cycle arrest and induced apoptosis of the bladder cancer cells. We also verified that daidzein efficiently suppressed RT112 cell xenograft tumor growth in nude mice. Mechanism studies indicated that significant down-regulation of the FGFR3 signaling pathway was responsible for the efficacy of daidzein. The phosphorylation levels of FGFR3, Akt and Erk proteins were suppressed in association with the decreasing of some apoptosis-suppressing molecules under the daidzein treatment. Knockdown of endogenous FGFR3 impaired the activity of daidzein against bladder cancer, which suggested that the effect of daidzein was mainly mediated by FGFR3 pathway. In addition, the function model of daidzein was similar with FGFR3 antagonist PD173074 in RT112 cells. Taken together, the results this study demonstrate that daidzein is capable of inhibiting bladder cancer growth and might be a novel effective chemotherapeutic agent for the application to combat bladder carcinoma.

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“…Some researchers have shown that daidzein has a positive effect on fat deposition. Zhao et al (2015) found that daidzein supplementation significantly increased the IMF content of crossbred steers. Li et al (2011) and Rehfeldt et al (2007) reported that adding a high dose of daidzein significantly increased the fat proportion in carcases of Guangxi minipig and piglets, respectively.…”

mentioning

confidence: 92%

“…Daidzein has been reported to be effective at affecting the meat quality of animals, but a few animal studies published have given conflicting results. Zhao et al (2015) found that daidzein supplementation improved the tenderness of beef by increasing the intramuscular fat (IMF) content and marbling score. On the other hand, Rehfeldt et al (2007) reported that supplemental daidzein changed the muscle fibre type in piglets.…”

Section: Introductionmentioning

confidence: 99%

Effects of daidzein on growth performance, blood metabolites and meat quality of finishing Xianan beef cattle

et al. 2019

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An experiment was conducted to determine the effects of supplementing different amounts of daidzein in a diet on the growth performance, blood biochemical parameters and meat quality of finishing beef cattle. Thirty finishing Xianan steers were distributed in three groups equilibrated by weight and fed three different dietary treatments (concentrate ratio = 80%): (1) control; (2) 500 mg/kg daidzein and (3) 1000 mg/kg daidzein, respectively. Steers were slaughtered after an 80-day feeding trial. Results showed that daidzein supplementation had no effect on the final body weight, average daily gain and feed conversion rate of steers. Steers fed with 1000 mg/kg daidzein had greater dry matter intake than those fed with control diets. Compared with the control group, the 1000 mg/kg daidzein group had a higher fat thickness, lower shear force and lightness. The pH, drip loss, cooking loss, redness (a*), yellowness (b*), moisture, ash, crude protein and intramuscular fat of the Longissimus dorsi muscle were unaffected by daidzein supplementation. Compared with the control group, the 1000 mg/kg daidzein group significantly increased the serum concentrations of insulin, free fatty acid and Glutamic-pyruvic transaminase. The 500 mg/kg daidzein group significantly increased the serum concentration of tetraiodothyronine compared with the control group. Supplemental daidzein did not affect the blood antioxidant ability and blood immune parameters in serum. In conclusion, daidzein supplementation above 500 mg/day modifies feed intake and metabolic and hormonal profile, with positive and negative effects on meat quality.

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Daidzein enhances intramuscular fat deposition and improves meat quality in finishing steers

Cited by 24 publications

References 36 publications

Saponin-Induced Shifts in the Rumen Microbiome and Metabolome of Young Cattle

Saponin-Induced Shifts in the Rumen Microbiome and Metabolome of Young Cattle

Daidzein exerts anti-tumor activity against bladder cancer cells via inhibition of FGFR3 pathway

Effects of daidzein on growth performance, blood metabolites and meat quality of finishing Xianan beef cattle

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