Daidzein induces choriocarcinoma cell apoptosis in a dose-dependent manner via the mitochondrial apoptotic pathway

Abstract

Choriocarcinoma is a malignant gestational trophoblastic disease and relapse or drug resistance occurs in ~25% of gestational trophoblastic tumors. Cell apoptosis serves a role in the progression from hydatidiform mole to persistent gestational trophoblastic disease. It has been demonstrated that daidzein [7‑hydroxy‑3‑(4‑hydroxyphenyl)‑4H‑chromen‑4‑one] may induce apoptosis in a number of cancer types via the mitochondrial apoptotic pathway by altering the B‑cell lymphoma (Bcl)‑2/Bcl‑2 associated X, apoptosis regulator (Bax) ratio, and activating the caspase cascade. Daidzein also serves a role in regulation of production of human chorionic gonadotropin in trophoblast cells and inhibition of cell proliferation. However, few reports have been published regarding the effect of daidzein on apoptosis in choriocarcinoma. Therefore, in the present study, JAR and JEG‑3 human gestational choriocarcinoma cells were used to investigate the effect of daidzein on apoptosis of choriocarcinoma cells. Treatment with daidzein for 48 h reduced cell viability in a dose‑dependent manner. The percentages of early and late apoptotic cells also increased following treatment with daidzein in a dose‑dependent manner, with the number of late apoptotic cells increasing more prominently. Furthermore, treatment with daidzein led to apoptosis‑associated alterations in nuclear morphology of JAR and JEG-3 cells. Expression levels of cleaved poly(ADP‑ribose) polymerase, cleaved caspase‑3 and cleaved caspase‑9 increased following treatment with daidzein, whereas the Bcl‑2/Bax ratio decreased in a dose‑dependent manner. In conclusion, the results of the present study demonstrate that daidzein may induce apoptosis of choriocarcinoma cells in a dose‑dependent manner via the mitochondrial apoptotic pathway.

Effect of fresh soy milk and its compounds on apoptosis in human leukemic cells and peripheral blood mononuclear cells

“…This could be because soy milk is rich in isoflavones, and may induce apoptosis unselectively. The present toxicity effect of coumestrol, daidzein and genistein to cancer cells was in good agreement with some previous studies which satisfactorily showed that high potential of phytoestrogens in killing cancer cells by the induction of apoptosis in prostate, hepatocellular carcinoma, bladder and colon cancer with various mechanism actions involved [19][20][21][22].…”

Protective Effect of Daidzein against Diethylnitrosamine/Carbon Tetrachloride-Induced Hepatocellular Carcinoma in Male Rats

“…Numerous studies indicate that DZ induces considerable anticancer action via the development of programmed cell death [ 45 ]. The compound has been reported to trigger apoptosis via the mitochondrial apoptotic pathway in various cancer types, such as breast cancer, gastric carcinoma, and hepatic cancer, by optimizing the Bcl-2/Bax ratio to activate the caspase cascade [ 46 ].…”

Investigation of the effect of steroidal imidazoheterocycles hormone derivatives on JEG-3 trophoblast cells of choriocarcinoma

“…Many natural and synthetic substances have been tested in the placental choriocarcinoma cell line. Some of them are daidzein, neuropeptide Y, fungicide ziram (CHEN et al, 2017;MATIĆ et al, 2017;ZHENG et al, 2018). There is an even larger number of studies on the influence of steroid hormones and their derivatives on the JEG-3 cell line, but also on the modulation of their synthesis (LONG et al, 1998;HONKISZ and WÓJTOWICZ, 2015;XU et al, 2019).…”