Daidzein induces neuritogenesis in DRG neuronal cultures

Abstract

AbsractBackgroundDaidzein, a phytoestrogen found in isoflavone, is known to exert neurotrophic and neuroprotective effects on the nervous system. Using primary rat dorsal root ganglion (DRG) neuronal cultures, we have examined the potential neurite outgrowth effect of daidzein.MethodsDissociated dorsal root ganglia (DRG) cultures were used to study the signaling mechanism of daidzein-induced neuritogenesis by immunocytochemistry and Western blotting.ResultsIn response to daidzein treatment, DRG neurons showed a significant increase in total neurite length and in tip number per neuron. The neuritogenic effect of daidzein was significantly hampered by specific blockers for Src, protein kinase C delta (PKCδ) and mitogen-activated protein kinase/extracellular signal-regulated kinase kinases (MEK/ERK), but not by those for estrogen receptor (ER). Moreover, daidzein induced phosphorylation of Src, PKCδ and ERK. The activation of PKCδ by daidzein was attenuated in the presence of a Src kinase inhibitor, and that of ERK by daidzein was diminished in the presence of either a Src or PKCδ inhibitor.ConclusionDaidzein may stimulate neurite outgrowth of DRG neurons depending on Src kinase, PKCδ and ERK signaling pathway.

The neuritogenic and synaptogenic effects of the ethanolic extract of radix Puerariae in cultured rat hippocampal neurons

“…Our findings concur with previous observations that puerarin protects against learning and memory in overiectomized (Ovx) mice, and that the beneficial effects of puerarin on improving memory behavior of Ovx mice are associated with synaptic structural modifications, which include an increase in postsynaptic protein PSD-95 content in Ovx mice following puerarin treatment (Xu and Zhang, 2007). Furthermore, several cellular experimental findings (Yang et al, 2012;Zhao et al, 2015) suggesting neuritogenesis, induced by puerarin and related small molecule compounds like daidzein, could be a general mechanism underlying the neurotrophic application are in good agreement to our results.…”

Botanical Drug Puerarin Coordinates with Nerve Growth Factor in the Regulation of Neuronal Survival and Neuritogenesis via Activating ERK1/2 and PI3K/Akt Signaling Pathways in the Neurite Extension Process

“…Natural products are increasingly recognized for the promotion of neuronal survival and neurogenesis against neuronal injury and chronic neurodegeneration . Isoflavones including daidzein and genistein are commonly classified as phytoestrogens with the potential to enhance NGF‐induced neurite outgrowth, although the underlying mechanisms remain elusive . After assaying puerarin, daidzein, genistein, calycosin, onion, and naringin in parallel, we found that puerarin, daidzein, and genistein could similarly potentiate NGF‐induced neurite outgrowth (Figure ).…”

Pueraria lobata and Daidzein Reduce Cytotoxicity by Enhancing Ubiquitin-Proteasome System Function in SCA3-iPSC-Derived Neurons

“…Isoflavonoids also have demonstrated prominent neuroprotective effects against cerebrovascular disorders, hypertension, or PD in rats or cell models [64–66]. One of its most abundant isoflavonoids, daidzein [67, 68], exerts neuroprotective effect to enhance neuronal survival and has been implicated to be a potential drug for neurodegenerative diseases [69–71]. Treatment with daidzein in PC12 cells reduced A β aggregates and the neuronal cytotoxicity of A β [69].…”