Daily humidity oscillation regulates the circadian clock to influence plant physiology

Mwimba, Musoki; Karapetyan, Sargis; Liu, Lijing; Marqués, Jorge; McGinnis, Erin; Buchler, Nicolas E.; Dong, Xinnian

doi:10.1038/s41467-018-06692-2

Cited by 49 publications

(39 citation statements)

References 63 publications

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“…Humidity fluctuations were random and small, varying between 39.4% and 46.2% (Fig. S6), which is much less than the 50–90% day/night oscillations of humidity shown to entrain the circadian oscillator in plants (Mwimba et al ., ). To confirm that the differences in leaf temperature were not an effect of fluctuations in chamber temperature, aluminium foil standards were placed next to the plants (as described in the Materials and Methods section).…”

Section: Resultsmentioning

confidence: 97%

Thermal imaging as a noninvasive technique for analyzing circadian rhythms in plants

Dakhiya

Green

2019

New Phytologist

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Endogenous (~24 circadian) rhythms control an enormously diverse range of processes in plants and are, increasingly, the target of studies aimed at understanding plant performance. Although in the previous few decades most plant circadian research has focused on Arabidopsis, there is a pressing need for low-cost, high-throughput tools for analyzing rhythms in a wider variety of species. The present contribution investigates using circadian temperature oscillations as a novel marker for assaying plant circadian rhythms.A thermal imaging platform was set up to measure diel and circadian rhythms in different plant species, in wild-type and circadian mutant plants, and in leaves and flowers. Results from the thermal imaging technique were compared with those from other established circadian assay techniques.All of the dicot and monocot species examined showed robust circadian rhythms of leaf surface temperature; the effects of circadian mutations on thermocycles were similar to those reported using other techniques. In Petunia 9 atkinsiana plants circadian oscillations were observed in both leaves and flowers.Thermal imaging is an extremely useful technique for analyzing circadian rhythms in plants. It is predicted that the ability to make very high temporal resolution measurements may facilitate the discovery of novel aspects of circadian control. conceived and devised the experiments and wrote the manuscript; and YD carried out the experiments. ORCIDRachel M. Green https://orcid.org/

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Section: Resultsmentioning

confidence: 97%

Thermal imaging as a noninvasive technique for analyzing circadian rhythms in plants

Dakhiya

Green

2019

New Phytologist

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“…It is possible that altering the circadian clock in these plants may lead to improper regulation of water intake, and thus cause the drought stress sensitivity phenotype. Alternatively, recent work demonstrates that humidity cycles are sufficient to entrain the plant circadian clock, and contributes to rhythmicity even under cycling light conditions (Mwimba et al, 2018). PUB48 may play a role in regulating this input pathway, and thus lead to both altered drought sensitivity and an altered circadian clock.…”

Section: Discussionmentioning

confidence: 99%

Decoys provide a scalable platform for the identification of plant E3 ubiquitin ligases that regulate circadian function

Feke

Liu

Hong

et al. 2019

eLife

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The circadian clock relies on regulated degradation of clock proteins to maintain rhythmicity. Despite this, we know few components that mediate protein degradation. This is due to high levels of functional redundancy within plant E3 ubiquitin ligase families. In order to overcome this issue and discover E3 ubiquitin ligases that control circadian function, we generated a library of transgenic Arabidopsis plants expressing dominant-negative ‘decoy’ E3 ubiquitin ligases. We determined their effects on the circadian clock and identified dozens of new potential regulators of circadian function. To demonstrate the potency of the decoy screening methodology to overcome redundancy and identify bona fide clock regulators, we performed follow-up studies on MAC3A (PUB59) and MAC3B (PUB60). We show that they redundantly control circadian period by regulating splicing. This work demonstrates the viability of ubiquitin ligase decoys as a screening platform to overcome genetic challenges and discover E3 ubiquitin ligases that regulate plant development.

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“…Daily oscillation of humidity also influences clock and immunity. Plant fitness and immunity are both enhanced under the day–night humidity‐changing conditions, compared with the constant humidity conditions (Mwimba et al ., ). Interestingly, humidity regulation of the clock and immunity seems to be distinct from that by light and temperature, given the retention of the former in a temperature‐insensitive clock mutant, prr7 prr9 .…”

Section: Environmental Effects On Plant Immunitymentioning

confidence: 99%

Plant immunity in signal integration between biotic and abiotic stress responses

2019

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Summary Plants constantly monitor and cope with the fluctuating environment while hosting a diversity of plant‐inhabiting microbes. The mode and outcome of plant–microbe interactions, including plant disease epidemics, are dynamically and profoundly influenced by abiotic factors, such as light, temperature, water and nutrients. Plants also utilize associations with beneficial microbes during adaptation to adverse conditions. Elucidation of the molecular bases for the plant–microbe–environment interactions is therefore of fundamental importance in the plant sciences. Following advances into individual stress signaling pathways, recent studies are beginning to reveal molecular intersections between biotic and abiotic stress responses and regulatory principles in combined stress responses. We outline mechanisms underlying environmental modulation of plant immunity and emerging roles for immune regulators in abiotic stress tolerance. Furthermore, we discuss how plants coordinate conflicting demands when exposed to combinations of different stresses, with attention to a possible determinant that links initial stress response to broad‐spectrum stress tolerance or prioritization of specific stress tolerance.

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Daily humidity oscillation regulates the circadian clock to influence plant physiology

Cited by 49 publications

References 63 publications

Thermal imaging as a noninvasive technique for analyzing circadian rhythms in plants

Thermal imaging as a noninvasive technique for analyzing circadian rhythms in plants

Decoys provide a scalable platform for the identification of plant E3 ubiquitin ligases that regulate circadian function

Plant immunity in signal integration between biotic and abiotic stress responses

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