Daily oscillation of gene expression associated with nacreous layer formation

Miyazaki, Yoko; Usui, Tomomi; Kajikawa, Aya; Hishiyama, Hajime; Matsuzawa, Norifumi; Nishida, Takuma; Machii, Akira; Samata, Tetsuro

doi:10.1007/s11706-008-0027-3

Cited by 12 publications

(5 citation statements)

References 11 publications

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“…(i) Restricted temporal expression [37], outside the 'window' of tissue sampling. Indeed, previous investigations have highlighted that key SMPs could be underrepresented or absent from a mantle EST database for such a reason [29].…”

Section: Shematrin-likementioning

confidence: 99%

Deep conservation of bivalve nacre proteins highlighted by shell matrix proteomics of the Unionoida Elliptio complanata and Villosa lienosa

Marie

Arivalagan

Matheron

et al. 2017

J. R. Soc. Interface.

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The formation of the molluscan shell nacre is regulated to a large extent by a matrix of extracellular macromolecules that are secreted by the shell-forming tissue, the mantle. This so-called ‘calcifying matrix’ is a complex mixture of proteins, glycoproteins and polysaccharides that is assembled and occluded within the mineral phase during the calcification process. Better molecular-level characterization of the substances that regulate nacre formation is still required. Notable advances in expressed tag sequencing of freshwater mussels, such as Elliptio complanata and Villosa lienosa , provide a pre-requisite to further characterize bivalve nacre proteins by a proteomic approach. In this study, we have identified a total of 48 different proteins from the insoluble matrices of the nacre, 31 of which are common to both E. complanata and V. lienosa . A few of these proteins, such as PIF, MSI60, CA, shematrin-like, Kunitz-like, LamG, chitin-binding-containing proteins, together with A-, D-, G-, M- and Q-rich proteins, appear to be analogues, if not true homologues, of proteins previously described from the pearl oyster or the edible mussel nacre matrices, thus forming a remarkable list of deeply conserved nacre proteins. This work constitutes a comprehensive nacre proteomic study of non-pteriomorphid bivalves that has enabled us to describe the molecular basis of a deeply conserved biomineralization toolkit among nacreous shell-bearing bivalves, with regard to proteins associated with other shell microstructures, with those of other mollusc classes (gastropods, cephalopods) and, finally, with other lophotrochozoans (brachiopods).

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Section: Shematrin-likementioning

confidence: 99%

Deep conservation of bivalve nacre proteins highlighted by shell matrix proteomics of the Unionoida Elliptio complanata and Villosa lienosa

Marie

Arivalagan

Matheron

et al. 2017

J. R. Soc. Interface.

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“…Differences have been reported in expression patterns of these genes encoding several shell matrix proteins between mantle and pearlsac epithelial cells, and among pearl-sac epithelial cells forming different types of pearls (Takeuchi and Endo 2005;Wang et al 2009;Inoue et al 2010Inoue et al , 2011aInoue et al , 2011b. Daily oscillation in the gene expression is also reported (Miyazaki et al 2008). However, there is still limited information on the relationships between morphology and gene expression of outer epithelial cells or pearl-sac epithelial cells.…”

Section: -4 Relationships Between Morphological Characteristics Of mentioning

confidence: 99%

Fundamental Studies oninvivoandinvitroPearl Formation—Contribution of Outer Epithelial Cells of Pearl Oyster Mantle and Pearl Sacs

Awaji¹,

Machii²

2011

Aqua-BioSci. Monogr. (ABSM)

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is the pallial line, which is an impression of the pallial muscles lined in an arc in the middle of a mantle. Several types of epithelial cells are recognized in the outer epithelium of a pearl oyster mantle (Tsujii 1960;Wada 1966). The most abundant type is named as outer epithelial cells. They are regarded to play a major role in shell formation and regeneration, and their functions are a main theme of this article.A shell of pearl oyster Pinctada fucata, characterized by its beautiful luster over the inner surface, consists of two layers: periostracum and ostracum. The periostracum is a thin, pliable proteinaceous sheet covering the outer surface of the shell. Components of the periostracum are synthesized in a periostracum gland located at the bottom of a periostracal groove between an outer and a middle fold of a mantle. The periostracum is thought to serve as a barrier that iso- Fundamental Studies on in vivo and in vitro AbstractOuter epithelial cells, which constitute a monolayer epithelium covering the outer surface of pearl oyster mantle, play principal roles in shell and pearl formation. In pearl culture, a fragment of the mantle prepared from a donor is implanted into the recipient's gonad together with a small inorganic bead. Histological studies using pearl oyster Pinctada fucata have revealed that the outer epithelial cells emigrate from the allograft, proliferate, and form a pearl sac surrounding the bead. Following the pearl-sac formation, the pearl-sac epithelia start to form calcium carbonate crystals, such as nacre, on the bead showing morphological characteristics closely related with the crystal structures.To investigate cellular mechanisms of the pearl formation, organ and cell culture methods for the outer epithelial cells of pearl oyster mantle were developed. In the organ culture, crystal formation, deposition of shell matrix-like structure, and DNA synthesis of the outer epithelial cells were observed. The outer epithelial cells separated from the mantle started DNA synthesis in co-culture with hemocytes that revealed a part of cellto-cell interactions during the pearl-sac formation processes. Substitution of the cultured outer epithelial cells for a mantle allograft in pearl culture was tested by injection of the cultured cells; the results of which implied future possibilities for the application of the cultured outer epithelial cells for pearl production.

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“…In the mantle of the pearl oyster P. fucata, the levels of expression of MSI60 and N16 genes were higher in winter season, while no seasonal variation was found in the Nacrein expression level (Miyazaki et al 2008). Nacrein contains two anhydrase carbonic anhydrase domains and GXN repeat domains, thus it is supposed to have a dual function in both CO 2 hydration and CaCO 3 interactions (Le Roy et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Expression of biomineralisation genes in tissues and cultured cells of the abalone Haliotis tuberculata

O'Neill¹,

Gaume²,

Denis³

et al. 2013

Cytotechnology

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Mollusc shell biomineralisation involves a variety of organic macromolecules (matrix proteins and enzymes) that control calcium carbonate (CaCO 3 ) deposition, growth of crystals, the selection of polymorph, and the microstructure of the shell. Since the mantle and the hemocytes play an important role in the control of shell formation, primary cell cultures have been developed to study the expression of three biomineralisation genes recently identified in the abalone Haliotis tuberculata: a matrix protein, Lustrin A, and two carbonic anhydrase enzymes. Mantle cells and hemocytes were successfully maintained in primary cultures and were evaluated for their viability and proliferation over time using a semi-automated assay (XTT). PCR and densitometric analysis were used to semi-quantify the gene expression and compare the level of expression in native tissues and cultured cells. The results demonstrated that the three genes of interest were being expressed in abalone tissues, with expression highest in the mantle and much lower in the hemocytes and the gills. Biomineralisation genes were also expressed significantly in mantle cells, confirming that primary cultures of target tissues are suitable models for in vitro investigation of matrix protein secretion.

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Daily oscillation of gene expression associated with nacreous layer formation

Cited by 12 publications

References 11 publications

Deep conservation of bivalve nacre proteins highlighted by shell matrix proteomics of the Unionoida Elliptio complanata and Villosa lienosa

Deep conservation of bivalve nacre proteins highlighted by shell matrix proteomics of the Unionoida Elliptio complanata and Villosa lienosa

Fundamental Studies oninvivoandinvitroPearl Formation—Contribution of Outer Epithelial Cells of Pearl Oyster Mantle and Pearl Sacs

Expression of biomineralisation genes in tissues and cultured cells of the abalone Haliotis tuberculata

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