Daily Rhythm of Serum Lipase and α-Amylase Activity in Fed and Fasted Dogs

Abstract

Abstract. The aim of the current study was to investigate the influence of feeding and fasting on the daily rhythm of serum lipase and a-amylase activity in dogs. Fourteen purebred Beagle dogs were housed in individual pens lined with wood shavings under a 12:12 light:dark cycle. The dogs were divided into 2 groups. Group A received normal feeding once a day, and group B was fasted starting 12 hr prior to the first blood collection. Water was available ad libitum. Blood samples were collected every 4 hr for 48 hr. Serum lipase and a-amylase activity were analyzed with standard kits by means of an ultraviolet spectrophotometer. Statistical analysis of the data was performed by 2-way analysis of variance and by single cosinor method. Results showed no statistical influence of feeding schedule on lipase and a-amylase and a robust daily rhythmicity of lipase and a-amylase in fed and fasted dogs. The current study could provide additional documentation of the structure of the dog circadian timing system and increase the necessary information related to the clinical approach to pancreatic diseases and to the therapeutic efficacy of timed administration of drugs or rations.

Amylase activity is associated with AMY2B copy numbers in dog: implications for dog domestication, diet and diabetes

“… 2000 ; Piccione et al . 2008 ; Nakajima et al . 2011a ) and the limited precision of qPCR to accurately resolve high copy number gene duplications (Hindson et al .…”

“…Second, and probably more important, serum amylase activity is influenced by dietary habits, age and circadian rhythm, factors that have not been taken into account in this study (Piccione et al . 2008 ). Although serum amylase activity clearly is associated with AMY2B copy number in dogs, care should thus be taken at this point not to interpret activity measures as a direct indicator of inherited ability to handle starch.…”

Scientific and technical assistance on welfare aspects related to housing and health of cats and dogs in commercial breeding establishments

“…Low-intensity dim red light can be, therefore, a useful tool for managing animals in the dark without impacting their welfare. Similarly to Bertolucci et al (2008), Piccione et al (2008) investigated the effects of the light cycle and two different feeding schedules on the daily rhythm of serum lipase and a-amylase in 14 purebred Beagles dogs. The dogs were housed in individual pens under the 12:12 light:dark schedule (light on at 08:00 am), with an illuminance of 600 lx (no further details on light type).…”

Comparison of rectal and vaginal temperature daily rhythm in dogs (Canis familiaris) under different photoperiod

“…In dog, daily rhythmicity has also been demonstrated in a variety of physiological functions, such as heart rate, blood pressure, bone metabolism, serum hormones and electrolytes, urine excretion, rest/activity, tear production and intraocular pressure and some metabolic parameters (Wong & Klein 1984;Gordon & Lavie 1985;Minten et al 1985;Nishimno et al 1997;Liesegang et al 1999;Piccione et al 2005Piccione et al , 2008Bertolucci et al 2008;Giudice et al 2009;, 2010.…”