Daily Timing of Mating and Age at Reproductive Maturity in Diaphorina citri (Hemiptera: Psyllidae)

Abstract

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Sampling Methods for Detection and Monitoring of the Asian Citrus Psyllid (Hemiptera: Psyllidae)

“…It should be noted that others have reported variation in engagement in mating behavior among D. citri males, including Wenninger & Hall (2007), who found that male latency to initiate calling significantly decreased and the numbers of calls detected in 10-m bioassays significantly increased with age. Zagvazdina et al (2015) found that mean responses of D. citri males in mate-seeking bioassays decreased when barometric pressure rose or fell more than 1 standard deviation over a 24-h period.…”

“…Thirty-three different males were tested during the (10 AM to 3 PM) period of greatest mating activity (Wenninger & Hall 2007). The handler placed the male onto a tree leaf using the walker tool described in Pregmon et al (2016).…”

Gross morphology and ultrastructure of the female reproductive system of Diaphorina citri (Hemiptera: Liviidae)

“…Last instar, newly-emerged, and reproductively active females of D. citri (WENNINGER & HALL 2007), at the previtellogenic and vitellogenic stages (DOSSI & CÔNSOLI 2010), were dissected in insect saline (3 mM CaCl 2 .2H 2 O, 182 mM KCl, 46 mM NaCl, 10 mM Tris base, pH 7.2) (CSH PROTOCOLS 2007). Ovaries were immediately transferred to the proper fixative solution for further processing.…”

Desenvolvimento ovariano e influência da cópula na maturação dos ovários de Diaphorina citri Kuwayama (Hemiptera: Psyllidae)

“…Adultos de D. citri recém-emergidos (≤ 12h) foram sexados, sendo machos e fêmeas mantidos separados em plantas de murta no interior de gaiolas de polietileno (30 cm altura x 10 cm diâmetro). Segundo Wenninger & Hall (2007), D. citri torna-se apta à cópula em torno de 2-3 dias após a emergência. Contudo, testes preliminares indicaram alta heterogeneidade quanto ao período de pré-cópula do inseto, optando-se por permitir a cópula apenas no décimo dia após a emergência.…”